Parthenogenetic Haemaphysalis longicornis acetylcholinesterases are triggered by the repellent effect of cinnamaldehyde, a primary compound found in cinnamon oil
The control and prevention of ticks and tick-borne diseases rely on chemical insecticides and repellents. Plant-derived compounds potentially represent new and safer repellents. Cinnamaldehyde, a component of cinnamon oil, exhibits antibacterial, anti-inflammatory, acaricidal, and repellent activity...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-11-01
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| Series: | Ticks and Tick-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X24000979 |
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| author | Ceyan kuang Jie Cao Yongzhi Zhou Houshuang Zhang Yanan Wang Jinlin Zhou |
| author_facet | Ceyan kuang Jie Cao Yongzhi Zhou Houshuang Zhang Yanan Wang Jinlin Zhou |
| author_sort | Ceyan kuang |
| collection | DOAJ |
| description | The control and prevention of ticks and tick-borne diseases rely on chemical insecticides and repellents. Plant-derived compounds potentially represent new and safer repellents. Cinnamaldehyde, a component of cinnamon oil, exhibits antibacterial, anti-inflammatory, acaricidal, and repellent activity against ticks. Here we studied the molecular mechanism of the repellent effect of cinnamaldehyde on Haemaphysalis longicornis. A 2 % cinnamaldehyde treatment resulted in >90 % nymph repellency within 6 h. Nymphs were exposed to cinnamaldehyde for 30 min, and subsequent transcriptome and metabolome analyses revealed the involvement of H. longicornis Acetylcholinesterases (HL-AchEs) in the response process. HL-AchEs was transcribed in all tick developmental stages and tissues. Following cinnamaldehyde treatment, the transcript and specific activity of the enzyme of AchE were significantly altered. Following RNAi, electroantennography (EAG) tests demonstrated a significant decrease in response to various repellents as well as a significant decrease in repellency. Our findings have revealed that HL-AchEs mediates cinnamaldehyde-induced tick repellency, and the results provide insights into the mechanism of plant-derived tick repellents. |
| format | Article |
| id | doaj-art-a6935f997c804251964907418aabb0c5 |
| institution | Kabale University |
| issn | 1877-9603 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj-art-a6935f997c804251964907418aabb0c52024-12-14T06:30:32ZengElsevierTicks and Tick-Borne Diseases1877-96032024-11-01156102404Parthenogenetic Haemaphysalis longicornis acetylcholinesterases are triggered by the repellent effect of cinnamaldehyde, a primary compound found in cinnamon oilCeyan kuang0Jie Cao1Yongzhi Zhou2Houshuang Zhang3Yanan Wang4Jinlin Zhou5Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR ChinaKey Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR ChinaKey Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR ChinaKey Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR ChinaCorrespondence authors.; Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR ChinaCorrespondence authors.; Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR ChinaThe control and prevention of ticks and tick-borne diseases rely on chemical insecticides and repellents. Plant-derived compounds potentially represent new and safer repellents. Cinnamaldehyde, a component of cinnamon oil, exhibits antibacterial, anti-inflammatory, acaricidal, and repellent activity against ticks. Here we studied the molecular mechanism of the repellent effect of cinnamaldehyde on Haemaphysalis longicornis. A 2 % cinnamaldehyde treatment resulted in >90 % nymph repellency within 6 h. Nymphs were exposed to cinnamaldehyde for 30 min, and subsequent transcriptome and metabolome analyses revealed the involvement of H. longicornis Acetylcholinesterases (HL-AchEs) in the response process. HL-AchEs was transcribed in all tick developmental stages and tissues. Following cinnamaldehyde treatment, the transcript and specific activity of the enzyme of AchE were significantly altered. Following RNAi, electroantennography (EAG) tests demonstrated a significant decrease in response to various repellents as well as a significant decrease in repellency. Our findings have revealed that HL-AchEs mediates cinnamaldehyde-induced tick repellency, and the results provide insights into the mechanism of plant-derived tick repellents.http://www.sciencedirect.com/science/article/pii/S1877959X24000979TickRepellentAcetylcholinesteraseTranscriptomeMetabolomeElectroantennography |
| spellingShingle | Ceyan kuang Jie Cao Yongzhi Zhou Houshuang Zhang Yanan Wang Jinlin Zhou Parthenogenetic Haemaphysalis longicornis acetylcholinesterases are triggered by the repellent effect of cinnamaldehyde, a primary compound found in cinnamon oil Ticks and Tick-Borne Diseases Tick Repellent Acetylcholinesterase Transcriptome Metabolome Electroantennography |
| title | Parthenogenetic Haemaphysalis longicornis acetylcholinesterases are triggered by the repellent effect of cinnamaldehyde, a primary compound found in cinnamon oil |
| title_full | Parthenogenetic Haemaphysalis longicornis acetylcholinesterases are triggered by the repellent effect of cinnamaldehyde, a primary compound found in cinnamon oil |
| title_fullStr | Parthenogenetic Haemaphysalis longicornis acetylcholinesterases are triggered by the repellent effect of cinnamaldehyde, a primary compound found in cinnamon oil |
| title_full_unstemmed | Parthenogenetic Haemaphysalis longicornis acetylcholinesterases are triggered by the repellent effect of cinnamaldehyde, a primary compound found in cinnamon oil |
| title_short | Parthenogenetic Haemaphysalis longicornis acetylcholinesterases are triggered by the repellent effect of cinnamaldehyde, a primary compound found in cinnamon oil |
| title_sort | parthenogenetic haemaphysalis longicornis acetylcholinesterases are triggered by the repellent effect of cinnamaldehyde a primary compound found in cinnamon oil |
| topic | Tick Repellent Acetylcholinesterase Transcriptome Metabolome Electroantennography |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X24000979 |
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