A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma
Cholangiocarcinoma is a primary malignant tumor of the bile duct epithelium. Cholangiocarcinoma is usually detected at an advanced stage when successful treatment is no longer possible. As the tumor originates from the bile duct epithelium, bile is an ideal source of tumor biomarkers for cholangioca...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-05-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317705764 |
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| author | Marut Laohaviroj Jeremy Potriquet Xinying Jia Sutas Suttiprapa Yaovalux Chamgramol Chawalit Pairojkul Paiboon Sithithaworn Jason Mulvenna Banchob Sripa |
| author_facet | Marut Laohaviroj Jeremy Potriquet Xinying Jia Sutas Suttiprapa Yaovalux Chamgramol Chawalit Pairojkul Paiboon Sithithaworn Jason Mulvenna Banchob Sripa |
| author_sort | Marut Laohaviroj |
| collection | DOAJ |
| description | Cholangiocarcinoma is a primary malignant tumor of the bile duct epithelium. Cholangiocarcinoma is usually detected at an advanced stage when successful treatment is no longer possible. As the tumor originates from the bile duct epithelium, bile is an ideal source of tumor biomarkers for cholangiocarcinoma. In this study, we used a quantitative proteomics approach to identify potential tumor-associated proteins in the bile fluid of six cholangiocarcinoma patients. Three different gross-appearance tumor types were used in the analysis: mass-forming type ( n = 2), periductal infiltrating type ( n = 2), and intraductal growth type ( n = 2). Two bile samples from non-cancerous patients were used as controls. Isobaric labeling, coupled with Tandem mass spectrometry, was used to quantify protein levels in the bile of cholangiocarcinoma and control patients. In all, 63 proteins were significantly increased in cholangiocarcinoma bile compared to normal bile. Alpha-1-antitrypsin was one of the overexpressed proteins that increased in cholangiocarcinoma bile samples. Immunohistochemical analysis revealed that alpha-1-antitrypsin was detected in 177 (50%) of 354 cholangiocarcinoma tissues from our Tissue Bank. Immunoblotting of 54 cholangiocarcinoma bile samples showed that alpha-1-antitrypsin was positive in 38 (70%) samples. Fecal enzyme-linked immunosorbent assay showed that alpha-1-antitrypsin level was able to distinguish cholangiocarcinoma patients from normal individuals. In conclusion, alpha-1-antitrypsin is a potential marker for early diagnosis of cholangiocarcinoma. |
| format | Article |
| id | doaj-art-a69309d9ad674c3fa53fcd089fd76c3b |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-a69309d9ad674c3fa53fcd089fd76c3b2025-08-20T03:20:27ZengSAGE PublishingTumor Biology1423-03802017-05-013910.1177/1010428317705764A comparative proteomic analysis of bile for biomarkers of cholangiocarcinomaMarut Laohaviroj0Jeremy Potriquet1Xinying Jia2Sutas Suttiprapa3Yaovalux Chamgramol4Chawalit Pairojkul5Paiboon Sithithaworn6Jason Mulvenna7Banchob Sripa8WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center (TDRC), Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandDepartment of Genetics and Computational Biology, QIMR Berghofer Medical Research Institute, Brisbane, QLD, AustraliaCentre for Advanced Imaging, The University of Queensland St Lucia, Brisbane, QLD, AustraliaWHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center (TDRC), Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandDepartment of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandDepartment of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandDepartment of Genetics and Computational Biology, QIMR Berghofer Medical Research Institute, Brisbane, QLD, AustraliaWHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center (TDRC), Faculty of Medicine, Khon Kaen University, Khon Kaen, ThailandCholangiocarcinoma is a primary malignant tumor of the bile duct epithelium. Cholangiocarcinoma is usually detected at an advanced stage when successful treatment is no longer possible. As the tumor originates from the bile duct epithelium, bile is an ideal source of tumor biomarkers for cholangiocarcinoma. In this study, we used a quantitative proteomics approach to identify potential tumor-associated proteins in the bile fluid of six cholangiocarcinoma patients. Three different gross-appearance tumor types were used in the analysis: mass-forming type ( n = 2), periductal infiltrating type ( n = 2), and intraductal growth type ( n = 2). Two bile samples from non-cancerous patients were used as controls. Isobaric labeling, coupled with Tandem mass spectrometry, was used to quantify protein levels in the bile of cholangiocarcinoma and control patients. In all, 63 proteins were significantly increased in cholangiocarcinoma bile compared to normal bile. Alpha-1-antitrypsin was one of the overexpressed proteins that increased in cholangiocarcinoma bile samples. Immunohistochemical analysis revealed that alpha-1-antitrypsin was detected in 177 (50%) of 354 cholangiocarcinoma tissues from our Tissue Bank. Immunoblotting of 54 cholangiocarcinoma bile samples showed that alpha-1-antitrypsin was positive in 38 (70%) samples. Fecal enzyme-linked immunosorbent assay showed that alpha-1-antitrypsin level was able to distinguish cholangiocarcinoma patients from normal individuals. In conclusion, alpha-1-antitrypsin is a potential marker for early diagnosis of cholangiocarcinoma.https://doi.org/10.1177/1010428317705764 |
| spellingShingle | Marut Laohaviroj Jeremy Potriquet Xinying Jia Sutas Suttiprapa Yaovalux Chamgramol Chawalit Pairojkul Paiboon Sithithaworn Jason Mulvenna Banchob Sripa A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma Tumor Biology |
| title | A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma |
| title_full | A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma |
| title_fullStr | A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma |
| title_full_unstemmed | A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma |
| title_short | A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma |
| title_sort | comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma |
| url | https://doi.org/10.1177/1010428317705764 |
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