D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathway
D-tryptophan is a special kind of nonprotein amino acid showing multiple physiological functions, but the detailed mechanisms are not fully revealed, impairing its further development and applications. This work was to investigate D-tryptophan physiological function and demonstrate the underlying me...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000520 |
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| _version_ | 1832557187744923648 |
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| author | Chong Wang Fangting Wang Yanbo Wang Linglin Fu |
| author_facet | Chong Wang Fangting Wang Yanbo Wang Linglin Fu |
| author_sort | Chong Wang |
| collection | DOAJ |
| description | D-tryptophan is a special kind of nonprotein amino acid showing multiple physiological functions, but the detailed mechanisms are not fully revealed, impairing its further development and applications. This work was to investigate D-tryptophan physiological function and demonstrate the underlying mechanisms. D-tryptophan suppressed HaCaT cell proliferation but increased cell migration. Specifically, D-tryptophan decreased E-cadherin and increased Snail, Twist, and Slug expression, resulting in the development of an epithelial-mesenchymal transitions (EMT) phenomenon. Moreover, D-tryptophan promoted the expression of transforming growth factor-β (TGF-β) 1, and Smad4 knockout damages D-tryptophan's ability. These results indicated that D-tryptophan stimulated HaCaT cells to produce TGF-β1 and thus activated the TGF-β/Samd pathway, resulting in the triggering of EMT. This study revealed the molecular mechanisms of D-tryptophan activity, provided D-tryptophan as a potential approach for cancer treatment, wound healing, organ development and other relevant applications. |
| format | Article |
| id | doaj-art-a692d4145b654c26b2f6f52ec634e7ba |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-a692d4145b654c26b2f6f52ec634e7ba2025-02-03T05:38:22ZengTsinghua University PressFood Science and Human Wellness2213-45302022-09-0111512151221D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathwayChong Wang0Fangting Wang1Yanbo Wang2Linglin Fu3Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaCorresponding author at: School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.; Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaD-tryptophan is a special kind of nonprotein amino acid showing multiple physiological functions, but the detailed mechanisms are not fully revealed, impairing its further development and applications. This work was to investigate D-tryptophan physiological function and demonstrate the underlying mechanisms. D-tryptophan suppressed HaCaT cell proliferation but increased cell migration. Specifically, D-tryptophan decreased E-cadherin and increased Snail, Twist, and Slug expression, resulting in the development of an epithelial-mesenchymal transitions (EMT) phenomenon. Moreover, D-tryptophan promoted the expression of transforming growth factor-β (TGF-β) 1, and Smad4 knockout damages D-tryptophan's ability. These results indicated that D-tryptophan stimulated HaCaT cells to produce TGF-β1 and thus activated the TGF-β/Samd pathway, resulting in the triggering of EMT. This study revealed the molecular mechanisms of D-tryptophan activity, provided D-tryptophan as a potential approach for cancer treatment, wound healing, organ development and other relevant applications.http://www.sciencedirect.com/science/article/pii/S2213453022000520D-tryptophanEpithelial-mesenchymal transitionsSmad4Transforming growth factor-β |
| spellingShingle | Chong Wang Fangting Wang Yanbo Wang Linglin Fu D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathway Food Science and Human Wellness D-tryptophan Epithelial-mesenchymal transitions Smad4 Transforming growth factor-β |
| title | D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathway |
| title_full | D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathway |
| title_fullStr | D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathway |
| title_full_unstemmed | D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathway |
| title_short | D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathway |
| title_sort | d tryptophan triggered epithelial mesenchymal transition by activating tgf β signaling pathway |
| topic | D-tryptophan Epithelial-mesenchymal transitions Smad4 Transforming growth factor-β |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000520 |
| work_keys_str_mv | AT chongwang dtryptophantriggeredepithelialmesenchymaltransitionbyactivatingtgfbsignalingpathway AT fangtingwang dtryptophantriggeredepithelialmesenchymaltransitionbyactivatingtgfbsignalingpathway AT yanbowang dtryptophantriggeredepithelialmesenchymaltransitionbyactivatingtgfbsignalingpathway AT linglinfu dtryptophantriggeredepithelialmesenchymaltransitionbyactivatingtgfbsignalingpathway |