Evaluation of the antidiabetic and antinephritic effects of Pueraria tuberosa and Pterocarpus santalinus extracts co-encapsulated in nanostructured lipid carriers in streptozotocin-induced mouse model
Background: Diabetic nephropathy (DN) causes significant morbidity and mortality, leading to substantial psycho-economic costs. New strategies and medications are needed to manage DN more effectively. Purpose: The antidiabetic and antinephritic properties of Pueraria tuberosa and Pterocarpus santali...
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Elsevier
2025-02-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031325000156 |
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| author | Gyamcho Tshering Bhutia Asit Kumar De Manas Bhowmik Pavia Bera Tanmoy Bera |
| author_facet | Gyamcho Tshering Bhutia Asit Kumar De Manas Bhowmik Pavia Bera Tanmoy Bera |
| author_sort | Gyamcho Tshering Bhutia |
| collection | DOAJ |
| description | Background: Diabetic nephropathy (DN) causes significant morbidity and mortality, leading to substantial psycho-economic costs. New strategies and medications are needed to manage DN more effectively. Purpose: The antidiabetic and antinephritic properties of Pueraria tuberosa and Pterocarpus santalinus have been demonstrated in previous studies. However, poor oral bioavailability has limited their clinical application. A nanostructured lipid carrier (NLC) formulation was created and tested to address this problem. The promising experimental results suggest that this formulation holds potential for further clinical exploration. Study design: The in vivo study evaluated the therapeutic potential of NLCs loaded with hydroalcoholic extracts of P. tuberosa tubers (PTE) and P. santalinus heartwood (PSE) in managing DN over eight weeks in streptozotocin (STZ)-induced diabetic mice. Methods: The protective effects of PTE and PSE were evaluated in mice at a dose of 80 mg/kg body weight. Two weeks after STZ treatment, animals with blood sugar levels above 250 mg/dL were identified as diabetic and showed signs of nephritis. In these diabetic mice, PTE-NLC and PSE-NLC, individually and in their combined form (PTE+PSE)-NLC, were assessed for their hypoglycemic and nephroprotective effects. Animals were sacrificed for histopathological and biochemical analysis after eight weeks of therapy. Nanoparticles were characterized by their morphology, zeta potential, drug loading capacity, particle size, and release profile. Results: In STZ-induced diabetic mice, treatment with (PTE+PSE)-NLC significantly improved pancreas and kidney health. It restored the kidney hypertrophy index, stabilized biochemical parameters, enhanced histopathological outcomes, and increased beta-cell count. Additionally, (PTE+PSE)-NLC led to a 30.53-fold more significant reduction in blood glucose levels than free (PTE+PSE) extracts. Conclusion: Our findings highlight the potential of the NLC formulation of P. tuberosa and P. santalinus extracts in treating diabetic nephropathy. The bioactive compounds in these extracts play a crucial role in their antidiabetic and antinephritic effects, marking significant progress in the field. |
| format | Article |
| id | doaj-art-a6906acad7c64a569054ba72a926cecf |
| institution | Kabale University |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Phytomedicine Plus |
| spelling | doaj-art-a6906acad7c64a569054ba72a926cecf2025-02-10T04:35:22ZengElsevierPhytomedicine Plus2667-03132025-02-0151100742Evaluation of the antidiabetic and antinephritic effects of Pueraria tuberosa and Pterocarpus santalinus extracts co-encapsulated in nanostructured lipid carriers in streptozotocin-induced mouse modelGyamcho Tshering Bhutia0Asit Kumar De1Manas Bhowmik2Pavia Bera3Tanmoy Bera4Laboratory of Nanomedicine, Division of Pharmaceutical Biotech., Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, West Bengal, IndiaDepartment of Chemistry, Jadavpur University, Kolkata 700032, West Bengal, IndiaPharmaceutics Research laboratory II, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, West Bengal, IndiaCollege of Engineering, 4202 East Fowler Avenue, ENG 030, University of South Florida, Tampa, FL 33620, USALaboratory of Nanomedicine, Division of Pharmaceutical Biotech., Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, West Bengal, India; Correspondence author at: Laboratory of Nanomedicine, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, West Bengal, India.Background: Diabetic nephropathy (DN) causes significant morbidity and mortality, leading to substantial psycho-economic costs. New strategies and medications are needed to manage DN more effectively. Purpose: The antidiabetic and antinephritic properties of Pueraria tuberosa and Pterocarpus santalinus have been demonstrated in previous studies. However, poor oral bioavailability has limited their clinical application. A nanostructured lipid carrier (NLC) formulation was created and tested to address this problem. The promising experimental results suggest that this formulation holds potential for further clinical exploration. Study design: The in vivo study evaluated the therapeutic potential of NLCs loaded with hydroalcoholic extracts of P. tuberosa tubers (PTE) and P. santalinus heartwood (PSE) in managing DN over eight weeks in streptozotocin (STZ)-induced diabetic mice. Methods: The protective effects of PTE and PSE were evaluated in mice at a dose of 80 mg/kg body weight. Two weeks after STZ treatment, animals with blood sugar levels above 250 mg/dL were identified as diabetic and showed signs of nephritis. In these diabetic mice, PTE-NLC and PSE-NLC, individually and in their combined form (PTE+PSE)-NLC, were assessed for their hypoglycemic and nephroprotective effects. Animals were sacrificed for histopathological and biochemical analysis after eight weeks of therapy. Nanoparticles were characterized by their morphology, zeta potential, drug loading capacity, particle size, and release profile. Results: In STZ-induced diabetic mice, treatment with (PTE+PSE)-NLC significantly improved pancreas and kidney health. It restored the kidney hypertrophy index, stabilized biochemical parameters, enhanced histopathological outcomes, and increased beta-cell count. Additionally, (PTE+PSE)-NLC led to a 30.53-fold more significant reduction in blood glucose levels than free (PTE+PSE) extracts. Conclusion: Our findings highlight the potential of the NLC formulation of P. tuberosa and P. santalinus extracts in treating diabetic nephropathy. The bioactive compounds in these extracts play a crucial role in their antidiabetic and antinephritic effects, marking significant progress in the field.http://www.sciencedirect.com/science/article/pii/S2667031325000156Diabetic nephropathyPueraria tuberosa extractPterocarpus santalinus extractBioavailabilityNanostructured lipid carrier systemHerbal compounds |
| spellingShingle | Gyamcho Tshering Bhutia Asit Kumar De Manas Bhowmik Pavia Bera Tanmoy Bera Evaluation of the antidiabetic and antinephritic effects of Pueraria tuberosa and Pterocarpus santalinus extracts co-encapsulated in nanostructured lipid carriers in streptozotocin-induced mouse model Phytomedicine Plus Diabetic nephropathy Pueraria tuberosa extract Pterocarpus santalinus extract Bioavailability Nanostructured lipid carrier system Herbal compounds |
| title | Evaluation of the antidiabetic and antinephritic effects of Pueraria tuberosa and Pterocarpus santalinus extracts co-encapsulated in nanostructured lipid carriers in streptozotocin-induced mouse model |
| title_full | Evaluation of the antidiabetic and antinephritic effects of Pueraria tuberosa and Pterocarpus santalinus extracts co-encapsulated in nanostructured lipid carriers in streptozotocin-induced mouse model |
| title_fullStr | Evaluation of the antidiabetic and antinephritic effects of Pueraria tuberosa and Pterocarpus santalinus extracts co-encapsulated in nanostructured lipid carriers in streptozotocin-induced mouse model |
| title_full_unstemmed | Evaluation of the antidiabetic and antinephritic effects of Pueraria tuberosa and Pterocarpus santalinus extracts co-encapsulated in nanostructured lipid carriers in streptozotocin-induced mouse model |
| title_short | Evaluation of the antidiabetic and antinephritic effects of Pueraria tuberosa and Pterocarpus santalinus extracts co-encapsulated in nanostructured lipid carriers in streptozotocin-induced mouse model |
| title_sort | evaluation of the antidiabetic and antinephritic effects of pueraria tuberosa and pterocarpus santalinus extracts co encapsulated in nanostructured lipid carriers in streptozotocin induced mouse model |
| topic | Diabetic nephropathy Pueraria tuberosa extract Pterocarpus santalinus extract Bioavailability Nanostructured lipid carrier system Herbal compounds |
| url | http://www.sciencedirect.com/science/article/pii/S2667031325000156 |
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