Strain-specific variations in the culture of chicken primordial germ cells
Abstract Efficient long-term cultivation of chicken primordial germ cells (cPGCs) is essential for various avian research and biotechnology applications. Our study aimed to address the challenge of inconsistent culture success by investigating strain-specific variations and optimizing culture condit...
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Nature Portfolio
2025-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-93777-w |
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| author | Sara Yousefi Taemeh Nima Dehdilani Lena Goshayeshi Clémence Kress Sylvie Rival-Gervier Guillaume Montillet Rouzbeh Ebrahimi Vishki Bertrand Pain Hesam Dehghani |
| author_facet | Sara Yousefi Taemeh Nima Dehdilani Lena Goshayeshi Clémence Kress Sylvie Rival-Gervier Guillaume Montillet Rouzbeh Ebrahimi Vishki Bertrand Pain Hesam Dehghani |
| author_sort | Sara Yousefi Taemeh |
| collection | DOAJ |
| description | Abstract Efficient long-term cultivation of chicken primordial germ cells (cPGCs) is essential for various avian research and biotechnology applications. Our study aimed to address the challenge of inconsistent culture success by investigating strain-specific variations and optimizing culture conditions using two distinct media: Ovotransferrin-enriched medium (OTM) and chicken serum-supplemented medium (CSM). We demonstrated that each chicken strain has unique nutritional requirements, with Hubbard cPGCs thriving in OTM and Bovans cPGCs favoring CSM. This strain-specific variation was effective in derivation and proliferation rates and the expression of stem cell-specific markers such as POU5F3/OCT4 and NANOG. Furthermore, our study confirmed the sustained germ cell identity of long-term cultured cPGCs through the expression of DAZL, DDX4, and EMA1 germ cell markers. We also showed that cultured cPGCs retained their migratory abilities and transfectability, successfully generating G0 germline chimeras and G1 transgenic Bovans chickens. These findings highlight the importance of optimized culture conditions depending on the genotype to enhance the viability and genetic stability of cPGCs, paving the way for more effective genetic modifications and conservation strategies in avian species. |
| format | Article |
| id | doaj-art-a68eec36f5444b998db074471d2e6911 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-a68eec36f5444b998db074471d2e69112025-08-20T03:06:51ZengNature PortfolioScientific Reports2045-23222025-04-0115111510.1038/s41598-025-93777-wStrain-specific variations in the culture of chicken primordial germ cellsSara Yousefi Taemeh0Nima Dehdilani1Lena Goshayeshi2Clémence Kress3Sylvie Rival-Gervier4Guillaume Montillet5Rouzbeh Ebrahimi Vishki6Bertrand Pain7Hesam Dehghani8Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of MashhadStem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of MashhadStem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of MashhadUniv Lyon, Université Lyon 1, INSERM, INRAE, Stem Cell and Brain Research Institute, U1208, USC1361Univ Lyon, Université Lyon 1, INSERM, INRAE, Stem Cell and Brain Research Institute, U1208, USC1361Univ Lyon, Université Lyon 1, INSERM, INRAE, Stem Cell and Brain Research Institute, U1208, USC1361Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of MashhadUniv Lyon, Université Lyon 1, INSERM, INRAE, Stem Cell and Brain Research Institute, U1208, USC1361Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of MashhadAbstract Efficient long-term cultivation of chicken primordial germ cells (cPGCs) is essential for various avian research and biotechnology applications. Our study aimed to address the challenge of inconsistent culture success by investigating strain-specific variations and optimizing culture conditions using two distinct media: Ovotransferrin-enriched medium (OTM) and chicken serum-supplemented medium (CSM). We demonstrated that each chicken strain has unique nutritional requirements, with Hubbard cPGCs thriving in OTM and Bovans cPGCs favoring CSM. This strain-specific variation was effective in derivation and proliferation rates and the expression of stem cell-specific markers such as POU5F3/OCT4 and NANOG. Furthermore, our study confirmed the sustained germ cell identity of long-term cultured cPGCs through the expression of DAZL, DDX4, and EMA1 germ cell markers. We also showed that cultured cPGCs retained their migratory abilities and transfectability, successfully generating G0 germline chimeras and G1 transgenic Bovans chickens. These findings highlight the importance of optimized culture conditions depending on the genotype to enhance the viability and genetic stability of cPGCs, paving the way for more effective genetic modifications and conservation strategies in avian species.https://doi.org/10.1038/s41598-025-93777-wChicken primordial germ cellsStrain-specific cultureGenetically-engineered birdsTransgenesis |
| spellingShingle | Sara Yousefi Taemeh Nima Dehdilani Lena Goshayeshi Clémence Kress Sylvie Rival-Gervier Guillaume Montillet Rouzbeh Ebrahimi Vishki Bertrand Pain Hesam Dehghani Strain-specific variations in the culture of chicken primordial germ cells Scientific Reports Chicken primordial germ cells Strain-specific culture Genetically-engineered birds Transgenesis |
| title | Strain-specific variations in the culture of chicken primordial germ cells |
| title_full | Strain-specific variations in the culture of chicken primordial germ cells |
| title_fullStr | Strain-specific variations in the culture of chicken primordial germ cells |
| title_full_unstemmed | Strain-specific variations in the culture of chicken primordial germ cells |
| title_short | Strain-specific variations in the culture of chicken primordial germ cells |
| title_sort | strain specific variations in the culture of chicken primordial germ cells |
| topic | Chicken primordial germ cells Strain-specific culture Genetically-engineered birds Transgenesis |
| url | https://doi.org/10.1038/s41598-025-93777-w |
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