Enhanced bacteriostatic effects of phage vB_C4 and cell wall-targeting antibiotic combinations against drug-resistant Aeromonas veronii
ABSTRACT Aeromonas veronii is a vital zoonotic pathogen known for its extensive drug resistance and ability to form biofilms, which contribute to its antibiotic resistance. In this study, the phage vB_C4, specifically targeting A. veronii, was isolated and subjected to bioinformatic analysis and bac...
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01908-24 |
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| author | Xin Cao Yanqiong Tang ZhenZhang Lu Xiang Ma Hong Li Xue Chi Juanjuan Li Zhu Liu |
| author_facet | Xin Cao Yanqiong Tang ZhenZhang Lu Xiang Ma Hong Li Xue Chi Juanjuan Li Zhu Liu |
| author_sort | Xin Cao |
| collection | DOAJ |
| description | ABSTRACT Aeromonas veronii is a vital zoonotic pathogen known for its extensive drug resistance and ability to form biofilms, which contribute to its antibiotic resistance. In this study, the phage vB_C4, specifically targeting A. veronii, was isolated and subjected to bioinformatic analysis and bacteriostatic activity assays. The combination of phage vB_C4 with antibiotics such as cephalothin and cefoxitin, which target the bacterial cell wall, resulted in a significantly enhanced bacteriostatic effect compared to either the phage or antibiotics alone. Furthermore, the phage dosage was critical in optimizing the antimicrobial effect when used in conjunction with antibiotics. This combined treatment exhibited a more distinct effect in removing mature biofilms and inhibiting biofilm formation, leading to a considerable decrease in bacterial density within the biofilm. Overall, the synergistic use of phage and antibiotics offers a novel attitude for treating pathogenic bacteria and holds significant potential in preventing the emergence of drug-resistant strains.IMPORTANCEThe combined application of phages and antibiotics not only effectively inhibits the emergence of phage-resistant bacteria but also reduces the required effective concentration of antibiotics. Additionally, this combination therapy demonstrates significant therapeutic effects on clinical infections mediated by biofilms. Consequently, this study establishes a basis for evaluating the parameters essential for utilizing phage-antibiotic combination therapy in the treatment of biofilm-associated infections, thereby offering a novel selection for the clinical management of multidrug-resistant bacterial infections. |
| format | Article |
| id | doaj-art-a68b54020c2b4713bfa0adb6db21e722 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-a68b54020c2b4713bfa0adb6db21e7222025-02-04T14:03:41ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.01908-24Enhanced bacteriostatic effects of phage vB_C4 and cell wall-targeting antibiotic combinations against drug-resistant Aeromonas veroniiXin Cao0Yanqiong Tang1ZhenZhang Lu2Xiang Ma3Hong Li4Xue Chi5Juanjuan Li6Zhu Liu7School of Life and Health Sciences, Hainan University, Haikou, ChinaSchool of Life and Health Sciences, Hainan University, Haikou, ChinaSchool of Life and Health Sciences, Hainan University, Haikou, ChinaSchool of Life and Health Sciences, Hainan University, Haikou, ChinaSchool of Life and Health Sciences, Hainan University, Haikou, ChinaSchool of Life and Health Sciences, Hainan University, Haikou, ChinaSchool of Life and Health Sciences, Hainan University, Haikou, ChinaYunnan Provincial Key Laboratory of Animal Nutrition and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaABSTRACT Aeromonas veronii is a vital zoonotic pathogen known for its extensive drug resistance and ability to form biofilms, which contribute to its antibiotic resistance. In this study, the phage vB_C4, specifically targeting A. veronii, was isolated and subjected to bioinformatic analysis and bacteriostatic activity assays. The combination of phage vB_C4 with antibiotics such as cephalothin and cefoxitin, which target the bacterial cell wall, resulted in a significantly enhanced bacteriostatic effect compared to either the phage or antibiotics alone. Furthermore, the phage dosage was critical in optimizing the antimicrobial effect when used in conjunction with antibiotics. This combined treatment exhibited a more distinct effect in removing mature biofilms and inhibiting biofilm formation, leading to a considerable decrease in bacterial density within the biofilm. Overall, the synergistic use of phage and antibiotics offers a novel attitude for treating pathogenic bacteria and holds significant potential in preventing the emergence of drug-resistant strains.IMPORTANCEThe combined application of phages and antibiotics not only effectively inhibits the emergence of phage-resistant bacteria but also reduces the required effective concentration of antibiotics. Additionally, this combination therapy demonstrates significant therapeutic effects on clinical infections mediated by biofilms. Consequently, this study establishes a basis for evaluating the parameters essential for utilizing phage-antibiotic combination therapy in the treatment of biofilm-associated infections, thereby offering a novel selection for the clinical management of multidrug-resistant bacterial infections.https://journals.asm.org/doi/10.1128/spectrum.01908-24bacteriostatic effectphage therapybioinformaticsantibioticsbiofilm elimination |
| spellingShingle | Xin Cao Yanqiong Tang ZhenZhang Lu Xiang Ma Hong Li Xue Chi Juanjuan Li Zhu Liu Enhanced bacteriostatic effects of phage vB_C4 and cell wall-targeting antibiotic combinations against drug-resistant Aeromonas veronii Microbiology Spectrum bacteriostatic effect phage therapy bioinformatics antibiotics biofilm elimination |
| title | Enhanced bacteriostatic effects of phage vB_C4 and cell wall-targeting antibiotic combinations against drug-resistant Aeromonas veronii |
| title_full | Enhanced bacteriostatic effects of phage vB_C4 and cell wall-targeting antibiotic combinations against drug-resistant Aeromonas veronii |
| title_fullStr | Enhanced bacteriostatic effects of phage vB_C4 and cell wall-targeting antibiotic combinations against drug-resistant Aeromonas veronii |
| title_full_unstemmed | Enhanced bacteriostatic effects of phage vB_C4 and cell wall-targeting antibiotic combinations against drug-resistant Aeromonas veronii |
| title_short | Enhanced bacteriostatic effects of phage vB_C4 and cell wall-targeting antibiotic combinations against drug-resistant Aeromonas veronii |
| title_sort | enhanced bacteriostatic effects of phage vb c4 and cell wall targeting antibiotic combinations against drug resistant aeromonas veronii |
| topic | bacteriostatic effect phage therapy bioinformatics antibiotics biofilm elimination |
| url | https://journals.asm.org/doi/10.1128/spectrum.01908-24 |
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