Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1
Abstract The salivary gland (SG) is vital for oral function and overall health through secretion of saliva. However salivary dysfunction due to aging, medications, autoimmune disorders, and cancer treatments poses significant challenges. We established the first diverse and clinically annotated sali...
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Nature Portfolio
2025-05-01
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| Series: | npj Regenerative Medicine |
| Online Access: | https://doi.org/10.1038/s41536-025-00410-5 |
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| author | Syed Mohammed Musheer Aalam Ana Rita Varela Aalim Khaderi Ronsard J. Mondesir Dong-Gi Mun Andrew Ding Isabelle M. A. Lombaert Rob P. Coppes Chitra Priya Emperumal Akhilesh Pandey Jeffrey R. Janus Nagarajan Kannan |
| author_facet | Syed Mohammed Musheer Aalam Ana Rita Varela Aalim Khaderi Ronsard J. Mondesir Dong-Gi Mun Andrew Ding Isabelle M. A. Lombaert Rob P. Coppes Chitra Priya Emperumal Akhilesh Pandey Jeffrey R. Janus Nagarajan Kannan |
| author_sort | Syed Mohammed Musheer Aalam |
| collection | DOAJ |
| description | Abstract The salivary gland (SG) is vital for oral function and overall health through secretion of saliva. However salivary dysfunction due to aging, medications, autoimmune disorders, and cancer treatments poses significant challenges. We established the first diverse and clinically annotated salivary regenerative biobank at Mayo Clinic to study salivary gland stem/progenitor cells (SGSPCs). Optimization of cell isolation and progenitor assays revealed SGSPCs enriched within the CD24/EpCAM/CD49f+ and PSMA- phenotypes of both submandibular and parotid glands, with clonal differentiation assays highlighting heterogeneity. Induction of PSMA/FOLH1 expression was associated with SGSPC differentiation. Using mass spectrometry-based single cell proteomics, we identified 2461 proteins in SGSPC-enriched cells, including co-expressed cytokeratins, expressed in rare salivary ductal basal cells. Additionally, PRDX, a unique class of peroxiredoxin peroxidases enriched in SGSPCs, demonstrated H2O2-dependent growth, suggesting a role in salivary homeostasis. These findings provide a foundation for SGSPC research and potential regenerative therapies for salivary gland dysfunction. |
| format | Article |
| id | doaj-art-a68964e01c5d4a299eabcad0da2ac23c |
| institution | OA Journals |
| issn | 2057-3995 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Regenerative Medicine |
| spelling | doaj-art-a68964e01c5d4a299eabcad0da2ac23c2025-08-20T01:52:25ZengNature Portfolionpj Regenerative Medicine2057-39952025-05-0110111910.1038/s41536-025-00410-5Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1Syed Mohammed Musheer Aalam0Ana Rita Varela1Aalim Khaderi2Ronsard J. Mondesir3Dong-Gi Mun4Andrew Ding5Isabelle M. A. Lombaert6Rob P. Coppes7Chitra Priya Emperumal8Akhilesh Pandey9Jeffrey R. Janus10Nagarajan Kannan11Department of Laboratory Medicine and Pathology, Mayo ClinicDepartment of Laboratory Medicine and Pathology, Mayo ClinicDepartment of Laboratory Medicine and Pathology, Mayo ClinicDepartment of Laboratory Medicine and Pathology, Mayo ClinicDepartment of Laboratory Medicine and Pathology, Mayo ClinicDepartment of Laboratory Medicine and Pathology, Mayo ClinicBiologic and Materials Sciences and Prosthodontics, University of Michigan School of Dentistry, 1011 N. University AveDepartments of Radiation Oncology and Biomedical Sciences, University of Groningen, University Medical Center GroningenDepartment of Laboratory Medicine and Pathology, Mayo ClinicDepartment of Laboratory Medicine and Pathology, Mayo ClinicDepartment of Otolaryngology-Head and Neck Surgery, Mayo ClinicDepartment of Laboratory Medicine and Pathology, Mayo ClinicAbstract The salivary gland (SG) is vital for oral function and overall health through secretion of saliva. However salivary dysfunction due to aging, medications, autoimmune disorders, and cancer treatments poses significant challenges. We established the first diverse and clinically annotated salivary regenerative biobank at Mayo Clinic to study salivary gland stem/progenitor cells (SGSPCs). Optimization of cell isolation and progenitor assays revealed SGSPCs enriched within the CD24/EpCAM/CD49f+ and PSMA- phenotypes of both submandibular and parotid glands, with clonal differentiation assays highlighting heterogeneity. Induction of PSMA/FOLH1 expression was associated with SGSPC differentiation. Using mass spectrometry-based single cell proteomics, we identified 2461 proteins in SGSPC-enriched cells, including co-expressed cytokeratins, expressed in rare salivary ductal basal cells. Additionally, PRDX, a unique class of peroxiredoxin peroxidases enriched in SGSPCs, demonstrated H2O2-dependent growth, suggesting a role in salivary homeostasis. These findings provide a foundation for SGSPC research and potential regenerative therapies for salivary gland dysfunction.https://doi.org/10.1038/s41536-025-00410-5 |
| spellingShingle | Syed Mohammed Musheer Aalam Ana Rita Varela Aalim Khaderi Ronsard J. Mondesir Dong-Gi Mun Andrew Ding Isabelle M. A. Lombaert Rob P. Coppes Chitra Priya Emperumal Akhilesh Pandey Jeffrey R. Janus Nagarajan Kannan Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1 npj Regenerative Medicine |
| title | Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1 |
| title_full | Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1 |
| title_fullStr | Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1 |
| title_full_unstemmed | Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1 |
| title_short | Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1 |
| title_sort | establishment of salivary tissue organoid biorepository characterizing salivary gland stem progenitor cells and novel differentiation marker psma folh1 |
| url | https://doi.org/10.1038/s41536-025-00410-5 |
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