Postbiotic Intervention in Sarcopenia: The Role of <i>Lactiplantibacillus plantarum</i> HY7715 and Its Extracellular Vesicles
Sarcopenia, the age-related loss of skeletal muscle mass and function, is associated with inflammation, mitochondrial dysfunction, and gut barrier impairment. This study investigates the postbiotic effects of heat-killed <i>Lactiplantibacillus plantarum</i> HY7715 (HY7715) and its extrac...
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MDPI AG
2025-07-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/15/7/1101 |
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| author | Kippeum Lee Soo Dong Park Joo Yun Kim Jae Jung Shim Jae Hwan Lee |
| author_facet | Kippeum Lee Soo Dong Park Joo Yun Kim Jae Jung Shim Jae Hwan Lee |
| author_sort | Kippeum Lee |
| collection | DOAJ |
| description | Sarcopenia, the age-related loss of skeletal muscle mass and function, is associated with inflammation, mitochondrial dysfunction, and gut barrier impairment. This study investigates the postbiotic effects of heat-killed <i>Lactiplantibacillus plantarum</i> HY7715 (HY7715) and its extracellular vesicles (EVs) on muscle health and intestinal integrity. In C2C12 myotubes, both treatments enhanced myogenic differentiation by upregulating Myf5 and MYOG, and improved mitochondrial activity and biogenesis via increased PGC1α and mTOR expression. Under TNFα-induced muscle atrophy, they suppressed expression of atrophy-related markers (Fbox32, MuRF1, and myostatin). EVs showed stronger anti-inflammatory effects by reducing IL6 expression in muscle cells. In intestinal Caco-2 cells, HY7715-derived EVs improved barrier function by upregulating tight junction proteins (ZO-1, occludin, and claudins), and effectively reduced LPS-induced inflammation. These findings suggest that heat-killed HY7715 and its EVs may alleviate sarcopenia by enhancing muscle regeneration and maintaining intestinal homeostasis, highlighting their potential as safe, gut–muscle axis-targeting postbiotic interventions for healthy aging. |
| format | Article |
| id | doaj-art-a684ae6e47874db595f0aa32bc36f065 |
| institution | DOAJ |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-a684ae6e47874db595f0aa32bc36f0652025-08-20T02:45:46ZengMDPI AGLife2075-17292025-07-01157110110.3390/life15071101Postbiotic Intervention in Sarcopenia: The Role of <i>Lactiplantibacillus plantarum</i> HY7715 and Its Extracellular VesiclesKippeum Lee0Soo Dong Park1Joo Yun Kim2Jae Jung Shim3Jae Hwan Lee4R&BD Center, Hy Co., Ltd., 22 Giheungdanji-ro 24 Beon-gil, Giheung-gu, Yongin-si 17086, Republic of KoreaR&BD Center, Hy Co., Ltd., 22 Giheungdanji-ro 24 Beon-gil, Giheung-gu, Yongin-si 17086, Republic of KoreaR&BD Center, Hy Co., Ltd., 22 Giheungdanji-ro 24 Beon-gil, Giheung-gu, Yongin-si 17086, Republic of KoreaR&BD Center, Hy Co., Ltd., 22 Giheungdanji-ro 24 Beon-gil, Giheung-gu, Yongin-si 17086, Republic of KoreaR&BD Center, Hy Co., Ltd., 22 Giheungdanji-ro 24 Beon-gil, Giheung-gu, Yongin-si 17086, Republic of KoreaSarcopenia, the age-related loss of skeletal muscle mass and function, is associated with inflammation, mitochondrial dysfunction, and gut barrier impairment. This study investigates the postbiotic effects of heat-killed <i>Lactiplantibacillus plantarum</i> HY7715 (HY7715) and its extracellular vesicles (EVs) on muscle health and intestinal integrity. In C2C12 myotubes, both treatments enhanced myogenic differentiation by upregulating Myf5 and MYOG, and improved mitochondrial activity and biogenesis via increased PGC1α and mTOR expression. Under TNFα-induced muscle atrophy, they suppressed expression of atrophy-related markers (Fbox32, MuRF1, and myostatin). EVs showed stronger anti-inflammatory effects by reducing IL6 expression in muscle cells. In intestinal Caco-2 cells, HY7715-derived EVs improved barrier function by upregulating tight junction proteins (ZO-1, occludin, and claudins), and effectively reduced LPS-induced inflammation. These findings suggest that heat-killed HY7715 and its EVs may alleviate sarcopenia by enhancing muscle regeneration and maintaining intestinal homeostasis, highlighting their potential as safe, gut–muscle axis-targeting postbiotic interventions for healthy aging.https://www.mdpi.com/2075-1729/15/7/1101postbiotics<i>Lactiplantibacillus plantarum</i> HY7715extracellular vesiclesarcopeniagut barrier impairment |
| spellingShingle | Kippeum Lee Soo Dong Park Joo Yun Kim Jae Jung Shim Jae Hwan Lee Postbiotic Intervention in Sarcopenia: The Role of <i>Lactiplantibacillus plantarum</i> HY7715 and Its Extracellular Vesicles Life postbiotics <i>Lactiplantibacillus plantarum</i> HY7715 extracellular vesicle sarcopenia gut barrier impairment |
| title | Postbiotic Intervention in Sarcopenia: The Role of <i>Lactiplantibacillus plantarum</i> HY7715 and Its Extracellular Vesicles |
| title_full | Postbiotic Intervention in Sarcopenia: The Role of <i>Lactiplantibacillus plantarum</i> HY7715 and Its Extracellular Vesicles |
| title_fullStr | Postbiotic Intervention in Sarcopenia: The Role of <i>Lactiplantibacillus plantarum</i> HY7715 and Its Extracellular Vesicles |
| title_full_unstemmed | Postbiotic Intervention in Sarcopenia: The Role of <i>Lactiplantibacillus plantarum</i> HY7715 and Its Extracellular Vesicles |
| title_short | Postbiotic Intervention in Sarcopenia: The Role of <i>Lactiplantibacillus plantarum</i> HY7715 and Its Extracellular Vesicles |
| title_sort | postbiotic intervention in sarcopenia the role of i lactiplantibacillus plantarum i hy7715 and its extracellular vesicles |
| topic | postbiotics <i>Lactiplantibacillus plantarum</i> HY7715 extracellular vesicle sarcopenia gut barrier impairment |
| url | https://www.mdpi.com/2075-1729/15/7/1101 |
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