Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women.
<h4>Background</h4>Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant's fasting status, m...
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Public Library of Science (PLoS)
2016-01-01
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| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0167832&type=printable |
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| author | Neil Murphy Roni T Falk Diana B Messinger Michael Pollak Xiaonan Xue Juan Lin Robin Sgueglia Howard D Strickler Mia M Gaudet Marc J Gunter |
| author_facet | Neil Murphy Roni T Falk Diana B Messinger Michael Pollak Xiaonan Xue Juan Lin Robin Sgueglia Howard D Strickler Mia M Gaudet Marc J Gunter |
| author_sort | Neil Murphy |
| collection | DOAJ |
| description | <h4>Background</h4>Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant's fasting status, may influence measurements.<h4>Methods</h4>We investigated the impact of fasting status and blood sample processing time on components of the insulin/IGF axis and in adipokines in a controlled feeding study of 45 healthy postmenopausal-women aged 50-75 years. Fasting blood samples were drawn (T0), after which subjects ate a standardized breakfast; subsequent blood draws were made at 1 hour (T1), 3 hours (T3), and 6 hours (T6) after breakfast. Serum samples were assayed for insulin, C-peptide, total- and free-IGF-I, IGF-binding protein [BP]-1 and -3, total and high molecular weight (HMW)-adiponectin, retinol binding protein-4, plasminogen activator inhibitor (PAI)-1, and resistin.<h4>Results</h4>Insulin and C-peptide levels followed similar postprandial trajectories; intra-class correlation coefficients [ICC] for insulin = 0.75, (95%CI:0.64-0.97) and C-peptide (ICC = 0.66, 95%CI:0.54-0.77) were similarly correlated in fasting (Spearman correlation, r = 0.78, 95%CI:0.64-0.88) and postprandial states (T1, r = 0.77 (95%CI: 0.62-0.87); T3,r = 0.78 (95%CI: 0.63-0.87); T6,r = 0.77 (95%CI: 0.61-0.87)). Free-IGF-I and IGFBP-1 levels were also affected by fasting status, whereas total-IGF-I and IGFBP-3 levels remained unchanged. Levels of adipokines were largely insensitive to fasting status and blood sample processing delays.<h4>Conclusion</h4>Several components of the insulin/IGF axis were significantly impacted by fasting state and in particular, C-peptide levels were substantially altered postprandially and in a similar manner to insulin. |
| format | Article |
| id | doaj-art-a67d5f5454214e7b8dd9ea3223cf6bd8 |
| institution | OA Journals |
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| language | English |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-a67d5f5454214e7b8dd9ea3223cf6bd82025-08-20T02:31:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016783210.1371/journal.pone.0167832Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women.Neil MurphyRoni T FalkDiana B MessingerMichael PollakXiaonan XueJuan LinRobin SguegliaHoward D StricklerMia M GaudetMarc J Gunter<h4>Background</h4>Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant's fasting status, may influence measurements.<h4>Methods</h4>We investigated the impact of fasting status and blood sample processing time on components of the insulin/IGF axis and in adipokines in a controlled feeding study of 45 healthy postmenopausal-women aged 50-75 years. Fasting blood samples were drawn (T0), after which subjects ate a standardized breakfast; subsequent blood draws were made at 1 hour (T1), 3 hours (T3), and 6 hours (T6) after breakfast. Serum samples were assayed for insulin, C-peptide, total- and free-IGF-I, IGF-binding protein [BP]-1 and -3, total and high molecular weight (HMW)-adiponectin, retinol binding protein-4, plasminogen activator inhibitor (PAI)-1, and resistin.<h4>Results</h4>Insulin and C-peptide levels followed similar postprandial trajectories; intra-class correlation coefficients [ICC] for insulin = 0.75, (95%CI:0.64-0.97) and C-peptide (ICC = 0.66, 95%CI:0.54-0.77) were similarly correlated in fasting (Spearman correlation, r = 0.78, 95%CI:0.64-0.88) and postprandial states (T1, r = 0.77 (95%CI: 0.62-0.87); T3,r = 0.78 (95%CI: 0.63-0.87); T6,r = 0.77 (95%CI: 0.61-0.87)). Free-IGF-I and IGFBP-1 levels were also affected by fasting status, whereas total-IGF-I and IGFBP-3 levels remained unchanged. Levels of adipokines were largely insensitive to fasting status and blood sample processing delays.<h4>Conclusion</h4>Several components of the insulin/IGF axis were significantly impacted by fasting state and in particular, C-peptide levels were substantially altered postprandially and in a similar manner to insulin.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0167832&type=printable |
| spellingShingle | Neil Murphy Roni T Falk Diana B Messinger Michael Pollak Xiaonan Xue Juan Lin Robin Sgueglia Howard D Strickler Mia M Gaudet Marc J Gunter Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women. PLoS ONE |
| title | Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women. |
| title_full | Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women. |
| title_fullStr | Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women. |
| title_full_unstemmed | Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women. |
| title_short | Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women. |
| title_sort | influence of fasting status and sample preparation on metabolic biomarker measurements in postmenopausal women |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0167832&type=printable |
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