Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in rats
Background: Methotrexate (MTX) is a commonly used antineoplastic and anti-rheumatoid agent whose efficacy is limited by marked organ toxicities associated with oxidative stress. The study investigated beneficial effect of virgin coconut oil (VCO) supplementation on MTX-induced oxidative stress and i...
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| Format: | Article |
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Elsevier
2018-09-01
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| Series: | Integrative Medicine Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213422018300337 |
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| author | Ademola C. Famurewa Abiola M. Folawiyo Elizabeth B. Enohnyaket Sharon O. Azubuike-Osu Innocent Abi Sunday G. Obaje Opeyemi A. Famurewa |
| author_facet | Ademola C. Famurewa Abiola M. Folawiyo Elizabeth B. Enohnyaket Sharon O. Azubuike-Osu Innocent Abi Sunday G. Obaje Opeyemi A. Famurewa |
| author_sort | Ademola C. Famurewa |
| collection | DOAJ |
| description | Background: Methotrexate (MTX) is a commonly used antineoplastic and anti-rheumatoid agent whose efficacy is limited by marked organ toxicities associated with oxidative stress. The study investigated beneficial effect of virgin coconut oil (VCO) supplementation on MTX-induced oxidative stress and inflammation in rats. Methods: Rats were divided into 4 groups (n = 6): Control, MTX (20 mg/kg bw), VCO (5%) + MTX and VCO (15%) + MTX. The pre-treatment with VCO for 14 days was followed by single intraperitoneal injection of MTX and the rats were sacrificed after 3 days. Serum activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and levels of reduced glutathione (GSH) and malondialdehyde (MDA) were determined. Interleukin-6 (IL-6), C-reactive protein (CRP) and nitric oxide (NO) levels were also evaluated. Results: MTX induced a distinct diminution in serum activities of oxidative stress markers (SOD, CAT, GPx and GSH), while lipid peroxidation considerably increased demonstrated by MDA level. Similarly, levels of IL-6, CRP and NO increased prominently in MTX control rats. The VCO supplementation markedly enhanced resistance to the MTX-induced biochemical alterations in rats. Conclusion: VCO can be a useful adjuvant natural product in MTX chemotherapy by reducing oxidative stress and pro-inflammatory responses. Keywords: Virgin coconut oil, Methotrexate, Supplementation, Oxidative stress, Inflammation |
| format | Article |
| id | doaj-art-a67ba452d5c64845839be8e24273f2cf |
| institution | OA Journals |
| issn | 2213-4220 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Integrative Medicine Research |
| spelling | doaj-art-a67ba452d5c64845839be8e24273f2cf2025-08-20T02:20:48ZengElsevierIntegrative Medicine Research2213-42202018-09-017325726310.1016/j.imr.2018.05.001Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in ratsAdemola C. Famurewa0Abiola M. Folawiyo1Elizabeth B. Enohnyaket2Sharon O. Azubuike-Osu3Innocent Abi4Sunday G. Obaje5Opeyemi A. Famurewa6Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Federal University, Ndufu-Alike, Ikwo, Abakaliki, Nigeria; Corresponding author at: Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria.Department of Physiology, College of Medicine, Ebonyi State University, Abakaliki, NigeriaDepartment of Physiology, College of Medicine, Ebonyi State University, Abakaliki, NigeriaDepartment of Physiology, Faculty of Basic Medical Sciences, Federal University, Ndufu-Alike, Ikwo, Abakaliki, NigeriaDepartment of Physiology, College of Health Sciences, Benue State University, Makurdi, NigeriaDepartment of Anatomy, Faculty of Basic Medical Sciences, Federal University, Ndufu-Alike, Ikwo, Abakaliki, NigeriaDepartment of Pharmacognosy and Traditional Medicine, Faculty of Pharmaceutical Science, University of Jos, Jos, NigeriaBackground: Methotrexate (MTX) is a commonly used antineoplastic and anti-rheumatoid agent whose efficacy is limited by marked organ toxicities associated with oxidative stress. The study investigated beneficial effect of virgin coconut oil (VCO) supplementation on MTX-induced oxidative stress and inflammation in rats. Methods: Rats were divided into 4 groups (n = 6): Control, MTX (20 mg/kg bw), VCO (5%) + MTX and VCO (15%) + MTX. The pre-treatment with VCO for 14 days was followed by single intraperitoneal injection of MTX and the rats were sacrificed after 3 days. Serum activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and levels of reduced glutathione (GSH) and malondialdehyde (MDA) were determined. Interleukin-6 (IL-6), C-reactive protein (CRP) and nitric oxide (NO) levels were also evaluated. Results: MTX induced a distinct diminution in serum activities of oxidative stress markers (SOD, CAT, GPx and GSH), while lipid peroxidation considerably increased demonstrated by MDA level. Similarly, levels of IL-6, CRP and NO increased prominently in MTX control rats. The VCO supplementation markedly enhanced resistance to the MTX-induced biochemical alterations in rats. Conclusion: VCO can be a useful adjuvant natural product in MTX chemotherapy by reducing oxidative stress and pro-inflammatory responses. Keywords: Virgin coconut oil, Methotrexate, Supplementation, Oxidative stress, Inflammationhttp://www.sciencedirect.com/science/article/pii/S2213422018300337 |
| spellingShingle | Ademola C. Famurewa Abiola M. Folawiyo Elizabeth B. Enohnyaket Sharon O. Azubuike-Osu Innocent Abi Sunday G. Obaje Opeyemi A. Famurewa Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in rats Integrative Medicine Research |
| title | Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in rats |
| title_full | Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in rats |
| title_fullStr | Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in rats |
| title_full_unstemmed | Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in rats |
| title_short | Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in rats |
| title_sort | beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy induced oxidative toxicity and inflammation in rats |
| url | http://www.sciencedirect.com/science/article/pii/S2213422018300337 |
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