Isolation, Culture, and Characterization of Primary Kidney Fibroblasts from Human Patients with Chronic Antibody-mediated Kidney Transplant Rejection

Isolation, Culture, and Characterization of Primary Kidney Fibroblasts from Human Patients with Chronic Antibody-mediated Kidney Transplant Rejection

Background: Inflammation and fibrosis are the primary occurrences of chronic antibody-mediated rejection (CABMR) in kidney transplant patients. Fibroblasts are the primary cell type involved in allograft rejection and play a crucial role in the pathogenesis and progression of chronic antibody-mediat...

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Bibliographic Details
Main Authors: Mantabya Kumar Singh, Mohit Kumar Rai, Vikas Agarwal, Narayan Prasad
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2024-12-01
Series:Indian Journal of Transplantation
Subjects:
collagen i
culture protocol
cytokeratin-8
e-cadherin
enzymatic digestion
immunofluorescence
kidney fibroblast
vimentin
α-smooth muscle actin
Online Access:https://journals.lww.com/10.4103/ijot.ijot_19_24
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