Transformation of CryK13V gene into protoplasts of Trichoderma viride
The lysine in site 13 of cryptogein protein was mutated to valine (K13V) through PCR site-directed mutagenesis. The mutant fragment (CryK13V) was confirmed by enzyme digestion and DNA sequencing. The CryK13V gene was expressed in Trichoderma viride, with constructed vector pCSNTCCm. Transformation w...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2006-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.03.0270 |
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