In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus
Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2014/383756 |
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| author | Duddi Narendra Nirupama Mohan Thomas Nainan Rajendran Ramaswamy Sethumadhavan Muralidharan Hulimangala Hosakote Lingareddy Usha Roshni Sharma Soham Gupta |
| author_facet | Duddi Narendra Nirupama Mohan Thomas Nainan Rajendran Ramaswamy Sethumadhavan Muralidharan Hulimangala Hosakote Lingareddy Usha Roshni Sharma Soham Gupta |
| author_sort | Duddi Narendra Nirupama |
| collection | DOAJ |
| description | Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms, E. faecalis, C. albicans, and S. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity against E. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity against C. albicans and S. aureus compared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity against E. faecalis, C. albicans, and S. aureus. |
| format | Article |
| id | doaj-art-a6561b7e87d24e62aeb12970d69ad653 |
| institution | OA Journals |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-a6561b7e87d24e62aeb12970d69ad6532025-08-20T02:20:49ZengWileyInternational Journal of Biomaterials1687-87871687-87952014-01-01201410.1155/2014/383756383756In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureusDuddi Narendra Nirupama0Mohan Thomas Nainan1Rajendran Ramaswamy2Sethumadhavan Muralidharan3Hulimangala Hosakote Lingareddy Usha4Roshni Sharma5Soham Gupta6Department of Conservative Dentistry & Endodontics, Vydehi Institute of Dental Sciences & Research Centre, No. 82, EPIP Area, Nallurahalli, Whitefield, Bangalore, Karnataka 560066, IndiaDepartment of Conservative Dentistry & Endodontics, Vydehi Institute of Dental Sciences & Research Centre, No. 82, EPIP Area, Nallurahalli, Whitefield, Bangalore, Karnataka 560066, IndiaDepartment of Microbiology, Vydehi Institute of Medical Sciences & Research Centre, No. 82, EPIP Area, Nallurahalli, Whitefield, Bangalore, Karnataka 560066, IndiaDepartment of Microbiology, St. John’s Medical College, Sarjapur Road, Bangalore, Karnataka 560034, IndiaDepartment of Conservative Dentistry & Endodontics, V. S. Dental College & Research Centre, Bangalore, Karnataka 560001, IndiaDepartment of Conservative Dentistry & Endodontics, Army College of Dental Sciences, Secunderabad 500087, IndiaDepartment of Microbiology, St. John’s Medical College, Sarjapur Road, Bangalore, Karnataka 560034, IndiaRoot canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms, E. faecalis, C. albicans, and S. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity against E. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity against C. albicans and S. aureus compared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity against E. faecalis, C. albicans, and S. aureus.http://dx.doi.org/10.1155/2014/383756 |
| spellingShingle | Duddi Narendra Nirupama Mohan Thomas Nainan Rajendran Ramaswamy Sethumadhavan Muralidharan Hulimangala Hosakote Lingareddy Usha Roshni Sharma Soham Gupta In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus International Journal of Biomaterials |
| title | In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus |
| title_full | In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus |
| title_fullStr | In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus |
| title_full_unstemmed | In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus |
| title_short | In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus |
| title_sort | in vitro evaluation of the antimicrobial efficacy of four endodontic biomaterials against enterococcus faecalis candida albicans and staphylococcus aureus |
| url | http://dx.doi.org/10.1155/2014/383756 |
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