Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti
Introduction: While the molecular epidemiology of extended-spectrum-b-lactamase (ESBL)-producing E. coli is well known in Europe due to effective surveillance networks and substantial literature, data for Africa are less available, especially in Djibouti. Methodology: We studied 31 isolates of E...
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The Journal of Infection in Developing Countries
2019-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/11283 |
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| author | Julie Plantamura Aurore Bousquet Serge Védy Sébastien Larréché Christine Bigaillon Hervé Delacour Audrey Mérens |
| author_facet | Julie Plantamura Aurore Bousquet Serge Védy Sébastien Larréché Christine Bigaillon Hervé Delacour Audrey Mérens |
| author_sort | Julie Plantamura |
| collection | DOAJ |
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Introduction: While the molecular epidemiology of extended-spectrum-b-lactamase (ESBL)-producing E. coli is well known in Europe due to effective surveillance networks and substantial literature, data for Africa are less available, especially in Djibouti.
Methodology: We studied 31 isolates of ESBL-producing E. coli from Djibouti and compared these molecular results with data available in Africa.
Results: Susceptibility rates were 3.2% for ceftazidim, 48.4% for piperacillin-tazobactam, 90.3% for amikacine and 16.1% for ofloxacin. No isolate showed resistance to carbapenems or colistin. 30 E. coli (96.8%) were positive to blaCTX-M-15, 1 (3.2%) to blaCTX-M-14 and 10 (32.3%) to narrow-broad-spectrum blaTEM. No blaSHV were detected. Fluoroquinolone resistance analysis showed that 30 ofloxacin-resistant E. coli had the mutation Ser-83->Leu on the gyrA gene. 24 E. coli (77.4%) harboured the plasmid-borne aac(6 ')-Ib-cr gene. No E. coli carried the genes qnrA, qnrB and qepA. 10 isolates (32.3%) belonging to the ST131 clone. The plasmid incompatibility group most widely represented in our collection was IncFIA/IB/II.
Conclusions: There is no major difference with African epidemiology. In particular, we notice the international diffusion of specific clonal group ST131.
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| format | Article |
| id | doaj-art-a64ae1bc3e08401d9708edd5adca2f39 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-a64ae1bc3e08401d9708edd5adca2f392025-08-20T02:57:21ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-08-01130810.3855/jidc.11283Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in DjiboutiJulie Plantamura0Aurore Bousquet1Serge Védy2Sébastien Larréché3Christine Bigaillon4Hervé Delacour5Audrey Mérens6Department of laboratory, Begin Teaching Military Hospital, Saint-Mandé, FranceDepartment of laboratory, Begin Teaching Military Hospital, Saint-Mandé, FranceDepartement of laboratory, Legouest Teaching Military Hospital, Metz, FranceDepartment of laboratory, Begin Teaching Military Hospital, Saint-Mandé, FranceDepartment of laboratory, Begin Teaching Military Hospital, Saint-Mandé, FranceDepartment of laboratory, Begin Teaching Military Hospital, Saint-Mandé, FranceDepartment of laboratory, Begin Teaching Military Hospital, Saint-Mandé, France Introduction: While the molecular epidemiology of extended-spectrum-b-lactamase (ESBL)-producing E. coli is well known in Europe due to effective surveillance networks and substantial literature, data for Africa are less available, especially in Djibouti. Methodology: We studied 31 isolates of ESBL-producing E. coli from Djibouti and compared these molecular results with data available in Africa. Results: Susceptibility rates were 3.2% for ceftazidim, 48.4% for piperacillin-tazobactam, 90.3% for amikacine and 16.1% for ofloxacin. No isolate showed resistance to carbapenems or colistin. 30 E. coli (96.8%) were positive to blaCTX-M-15, 1 (3.2%) to blaCTX-M-14 and 10 (32.3%) to narrow-broad-spectrum blaTEM. No blaSHV were detected. Fluoroquinolone resistance analysis showed that 30 ofloxacin-resistant E. coli had the mutation Ser-83->Leu on the gyrA gene. 24 E. coli (77.4%) harboured the plasmid-borne aac(6 ')-Ib-cr gene. No E. coli carried the genes qnrA, qnrB and qepA. 10 isolates (32.3%) belonging to the ST131 clone. The plasmid incompatibility group most widely represented in our collection was IncFIA/IB/II. Conclusions: There is no major difference with African epidemiology. In particular, we notice the international diffusion of specific clonal group ST131. https://jidc.org/index.php/journal/article/view/11283E. coliESBLAfricaDjiboutiST131 |
| spellingShingle | Julie Plantamura Aurore Bousquet Serge Védy Sébastien Larréché Christine Bigaillon Hervé Delacour Audrey Mérens Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti Journal of Infection in Developing Countries E. coli ESBL Africa Djibouti ST131 |
| title | Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti |
| title_full | Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti |
| title_fullStr | Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti |
| title_full_unstemmed | Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti |
| title_short | Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti |
| title_sort | molecular epidemiological of extended spectrum β lactamase producing escherichia coli isolated in djibouti |
| topic | E. coli ESBL Africa Djibouti ST131 |
| url | https://jidc.org/index.php/journal/article/view/11283 |
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