A beginners guide to Sf9 and Sf21 insect cell line culture and troubleshooting

Abstract Recombinant proteins are not only a crucial research tool but are also widely implemented in biomedicine. There are a number of expression systems used for recombinant protein production. Among them Spodoptera frugiperda (Sf) insect cell system is a powerful tool for multiprotein expression...

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Main Authors: Joanna Kwiatkowska, Ewa Stein, Anastasiia Romanenko, Martyna Plens-Gałąska, Małgorzata Podsiadła-Białoskórska, Ewa Szołajska, Uwe Kühn, Kinga Kamieniarz-Gdula
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-99812-0
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