Cryo‐EM Analysis of a Tri‐Heme Cytochrome‐Associated RC‐LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae
Abstract The reaction center‐light harvesting 1 (RC‐LH1) complex converts solar energy into electrical energy, driving the initiation of photosynthesis. The authors present a cryo‐electron microscopy structure of the RC‐LH1 isolated from a marine photoheterotrophic bacterium Dinoroseobacter shibae....
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Wiley
2025-05-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202413456 |
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| author | Weiwei Wang Yanting Liu Jiayi Gu Shaoya An Cheng Ma Haichun Gao Nianzhi Jiao Jian‐Ren Shen John Thomas Beatty Michal Koblížek Xing Zhang Qiang Zheng Jing‐Hua Chen |
| author_facet | Weiwei Wang Yanting Liu Jiayi Gu Shaoya An Cheng Ma Haichun Gao Nianzhi Jiao Jian‐Ren Shen John Thomas Beatty Michal Koblížek Xing Zhang Qiang Zheng Jing‐Hua Chen |
| author_sort | Weiwei Wang |
| collection | DOAJ |
| description | Abstract The reaction center‐light harvesting 1 (RC‐LH1) complex converts solar energy into electrical energy, driving the initiation of photosynthesis. The authors present a cryo‐electron microscopy structure of the RC‐LH1 isolated from a marine photoheterotrophic bacterium Dinoroseobacter shibae. The RC comprises four subunits, including a three‐heme cytochrome (Cyt) c protein, and is surrounded by a closed LH ring composed of 17 pairs of antenna subunits. Notably, a novel subunit with an N‐terminal “helix‐turn‐helix” motif embedded in the gap between the RC and the LH ring is identified. The purified RC‐LH1 complex exhibits high stability in solutions containing Mg2+ or Ca2+. The periplasmic Cyt c2 is predicted to bind at the junction between the Cyt subunit and the membrane plane, enabling electron transfer from Cyt c2 to the proximal heme of the tri‐heme Cyt, and subsequently to the special pair of bacteriochlorophylls. These findings provide structural insights into the efficient energy and electron transfer processes within a distinct type of RC‐LH1, and shed light on evolutionary adaptations of photosynthesis. |
| format | Article |
| id | doaj-art-a641f43bbc614bfa914bf95bb99425f0 |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
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| series | Advanced Science |
| spelling | doaj-art-a641f43bbc614bfa914bf95bb99425f02025-08-20T03:11:14ZengWileyAdvanced Science2198-38442025-05-011218n/an/a10.1002/advs.202413456Cryo‐EM Analysis of a Tri‐Heme Cytochrome‐Associated RC‐LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter ShibaeWeiwei Wang0Yanting Liu1Jiayi Gu2Shaoya An3Cheng Ma4Haichun Gao5Nianzhi Jiao6Jian‐Ren Shen7John Thomas Beatty8Michal Koblížek9Xing Zhang10Qiang Zheng11Jing‐Hua Chen12College of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 ChinaState Key Laboratory of Marine Environmental Science College of Ocean and Earth Sciences Xiamen University Xiamen Fujian 361005 ChinaCollege of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 ChinaDepartment of Pathology of Sir Run Run Shaw Hospital Department of Biophysics Zhejiang University School of Medicine Hangzhou Zhejiang 310058 ChinaDepartment of Pathology of Sir Run Run Shaw Hospital Department of Biophysics Zhejiang University School of Medicine Hangzhou Zhejiang 310058 ChinaCollege of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 ChinaState Key Laboratory of Marine Environmental Science College of Ocean and Earth Sciences Xiamen University Xiamen Fujian 361005 ChinaPhotosynthesis Research Center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 ChinaDepartment of Microbiology & Immunology University of British Columbia Vancouver BC V6T 1Z3 CanadaLaboratory of Anoxygenic Phototrophs Institute of Microbiology Czech Academy of Science Třeboň 37981 CzechiaDepartment of Pathology of Sir Run Run Shaw Hospital Department of Biophysics Zhejiang University School of Medicine Hangzhou Zhejiang 310058 ChinaState Key Laboratory of Marine Environmental Science College of Ocean and Earth Sciences Xiamen University Xiamen Fujian 361005 ChinaCollege of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 ChinaAbstract The reaction center‐light harvesting 1 (RC‐LH1) complex converts solar energy into electrical energy, driving the initiation of photosynthesis. The authors present a cryo‐electron microscopy structure of the RC‐LH1 isolated from a marine photoheterotrophic bacterium Dinoroseobacter shibae. The RC comprises four subunits, including a three‐heme cytochrome (Cyt) c protein, and is surrounded by a closed LH ring composed of 17 pairs of antenna subunits. Notably, a novel subunit with an N‐terminal “helix‐turn‐helix” motif embedded in the gap between the RC and the LH ring is identified. The purified RC‐LH1 complex exhibits high stability in solutions containing Mg2+ or Ca2+. The periplasmic Cyt c2 is predicted to bind at the junction between the Cyt subunit and the membrane plane, enabling electron transfer from Cyt c2 to the proximal heme of the tri‐heme Cyt, and subsequently to the special pair of bacteriochlorophylls. These findings provide structural insights into the efficient energy and electron transfer processes within a distinct type of RC‐LH1, and shed light on evolutionary adaptations of photosynthesis.https://doi.org/10.1002/advs.202413456energy transferphotoheterotrophic bacteriaphotosynthesisreaction centerstructure |
| spellingShingle | Weiwei Wang Yanting Liu Jiayi Gu Shaoya An Cheng Ma Haichun Gao Nianzhi Jiao Jian‐Ren Shen John Thomas Beatty Michal Koblížek Xing Zhang Qiang Zheng Jing‐Hua Chen Cryo‐EM Analysis of a Tri‐Heme Cytochrome‐Associated RC‐LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae Advanced Science energy transfer photoheterotrophic bacteria photosynthesis reaction center structure |
| title | Cryo‐EM Analysis of a Tri‐Heme Cytochrome‐Associated RC‐LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae |
| title_full | Cryo‐EM Analysis of a Tri‐Heme Cytochrome‐Associated RC‐LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae |
| title_fullStr | Cryo‐EM Analysis of a Tri‐Heme Cytochrome‐Associated RC‐LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae |
| title_full_unstemmed | Cryo‐EM Analysis of a Tri‐Heme Cytochrome‐Associated RC‐LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae |
| title_short | Cryo‐EM Analysis of a Tri‐Heme Cytochrome‐Associated RC‐LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae |
| title_sort | cryo em analysis of a tri heme cytochrome associated rc lh1 complex from the marine photoheterotrophic bacterium dinoroseobacter shibae |
| topic | energy transfer photoheterotrophic bacteria photosynthesis reaction center structure |
| url | https://doi.org/10.1002/advs.202413456 |
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