Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity
Objective Helicobacter pylori infection is acquired in childhood via the oral cavity, although its relationship with the characteristics of the oral microbiome has not been elucidated. In this study, we performed comprehensive analysis of the oral microbiome in children and adults with or without H....
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2024.2339158 |
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| author | Yuko Ogaya Tamami Kadota Masakazu Hamada Ryota Nomura Kazuhiko Nakano |
| author_facet | Yuko Ogaya Tamami Kadota Masakazu Hamada Ryota Nomura Kazuhiko Nakano |
| author_sort | Yuko Ogaya |
| collection | DOAJ |
| description | Objective Helicobacter pylori infection is acquired in childhood via the oral cavity, although its relationship with the characteristics of the oral microbiome has not been elucidated. In this study, we performed comprehensive analysis of the oral microbiome in children and adults with or without H. pylori in the oral cavity.Methods Bacterial DNA was extracted from 41 adult and 21 child saliva specimens, and H. pylori was detected using PCR. 16S rRNA gene amplification was performed for next-generation sequencing. Bioinformatic analyses were conducted using Quantitative Insights into Microbial Ecology 2 (QIIME 2).Results Faith’s phylogenetic diversity analysis showed a significant difference between H. pylori-negative adult and child specimens in terms of α-diversity (p < 0.05), while no significant difference was observed between H. pylori-positive adult and child specimens. There was also a significant difference in β-diversity between H. pylori-positive and negative child specimens (p < 0.05). Taxonomic analysis at the genus level revealed that Porphyromonas was the only bacterium that was significantly more abundant in both H. pylori-positive adults and children than in corresponding negative specimens (p < 0.01 and p < 0.05, respectively).Conclusion These results suggest unique oral microbiome characteristics in children with H. pylori infection in the oral cavity. |
| format | Article |
| id | doaj-art-a641a2c7f99f48d6ba504c0332686d55 |
| institution | OA Journals |
| issn | 2000-2297 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-a641a2c7f99f48d6ba504c0332686d552025-08-20T01:53:15ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972024-12-0116110.1080/20002297.2024.2339158Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavityYuko Ogaya0Tamami Kadota1Masakazu Hamada2Ryota Nomura3Kazuhiko Nakano4Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, JapanDepartment of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, JapanDepartment of Oral & Maxillofacial Oncology and Surgery, Osaka University Graduate School of Dentistry, Osaka, JapanDepartment of Pediatric Dentistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, JapanDepartment of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, JapanObjective Helicobacter pylori infection is acquired in childhood via the oral cavity, although its relationship with the characteristics of the oral microbiome has not been elucidated. In this study, we performed comprehensive analysis of the oral microbiome in children and adults with or without H. pylori in the oral cavity.Methods Bacterial DNA was extracted from 41 adult and 21 child saliva specimens, and H. pylori was detected using PCR. 16S rRNA gene amplification was performed for next-generation sequencing. Bioinformatic analyses were conducted using Quantitative Insights into Microbial Ecology 2 (QIIME 2).Results Faith’s phylogenetic diversity analysis showed a significant difference between H. pylori-negative adult and child specimens in terms of α-diversity (p < 0.05), while no significant difference was observed between H. pylori-positive adult and child specimens. There was also a significant difference in β-diversity between H. pylori-positive and negative child specimens (p < 0.05). Taxonomic analysis at the genus level revealed that Porphyromonas was the only bacterium that was significantly more abundant in both H. pylori-positive adults and children than in corresponding negative specimens (p < 0.01 and p < 0.05, respectively).Conclusion These results suggest unique oral microbiome characteristics in children with H. pylori infection in the oral cavity.https://www.tandfonline.com/doi/10.1080/20002297.2024.2339158Helicobacter pylorioral microbiomeoral cavitysalivametagenomic analysis |
| spellingShingle | Yuko Ogaya Tamami Kadota Masakazu Hamada Ryota Nomura Kazuhiko Nakano Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity Journal of Oral Microbiology Helicobacter pylori oral microbiome oral cavity saliva metagenomic analysis |
| title | Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity |
| title_full | Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity |
| title_fullStr | Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity |
| title_full_unstemmed | Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity |
| title_short | Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity |
| title_sort | characterization of the unique oral microbiome of children harboring helicobacter pylori in the oral cavity |
| topic | Helicobacter pylori oral microbiome oral cavity saliva metagenomic analysis |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2024.2339158 |
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