Identification of Anti-Inflammatory Components from <i>Launaea sarmentosa</i> Using <i>In Vitro</i> Cell Model
Launaea sarmentosa (Willd.) Kuntze, a medicinal herb known for treating inflammatory diseases, was examined for its anti-inflammatory compounds to identify novel therapies. This study indicated that hexane and ethyl acetate fractional extracts significantly reduced NO secretion in LPS-stimulated RAW...
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Universitas Gadjah Mada
2025-07-01
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| Series: | Indonesian Journal of Chemistry |
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| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/103167 |
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| author | Thanh Quoc Chau Nguyen Khang Thanh Vo Cua Dinh Duong Toan Phu Huynh Lieu Thi Thuy Ca Giao Huynh Dang |
| author_facet | Thanh Quoc Chau Nguyen Khang Thanh Vo Cua Dinh Duong Toan Phu Huynh Lieu Thi Thuy Ca Giao Huynh Dang |
| author_sort | Thanh Quoc Chau Nguyen |
| collection | DOAJ |
| description | Launaea sarmentosa (Willd.) Kuntze, a medicinal herb known for treating inflammatory diseases, was examined for its anti-inflammatory compounds to identify novel therapies. This study indicated that hexane and ethyl acetate fractional extracts significantly reduced NO secretion in LPS-stimulated RAW264.7 macrophages, indicating the presence of potential anti-inflammatory compounds. Additionally, four anti-inflammatory compounds, including taraxasteryl acetate (1), esculetin (2), pyrimidine-2,4-dione (3), and 5-hydroxypryrolidin-2-one (4), were isolated, and their structures were characterized using 1D and 2D-NMR. This study marked the first report of taraxasteryl acetate, pyrimidine-2,4-dione, and 5-hydroxypryrolidin-2-one being isolated from this species. Furthermore, these compounds exerted their anti-inflammatory role by inhibiting NO production and TNF-α expression. Thus, this study contributes to identifying anti-inflammatory constituents from L. sarmentosa and highlights a potential approach for developing phytotherapeutic agents. |
| format | Article |
| id | doaj-art-a6258fb7f16d4f219ea134951d8b9dcc |
| institution | DOAJ |
| issn | 1411-9420 2460-1578 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj-art-a6258fb7f16d4f219ea134951d8b9dcc2025-08-20T03:01:41ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782025-07-012541077108610.22146/ijc.10316737781Identification of Anti-Inflammatory Components from <i>Launaea sarmentosa</i> Using <i>In Vitro</i> Cell ModelThanh Quoc Chau Nguyen0Khang Thanh Vo1Cua Dinh Duong2Toan Phu Huynh3Lieu Thi Thuy Ca4Giao Huynh Dang5Department of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, VietnamDepartment of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam; Medicinal Chemistry Laboratory, CTU Hi-tech Building, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, VietnamDepartment of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam; Medicinal Chemistry Laboratory, CTU Hi-tech Building, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, VietnamDepartment of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam; Medicinal Chemistry Laboratory, CTU Hi-tech Building, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, VietnamMedicinal Chemistry Laboratory, CTU Hi-tech Building, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, VietnamCollege of Engineering, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, VietnamLaunaea sarmentosa (Willd.) Kuntze, a medicinal herb known for treating inflammatory diseases, was examined for its anti-inflammatory compounds to identify novel therapies. This study indicated that hexane and ethyl acetate fractional extracts significantly reduced NO secretion in LPS-stimulated RAW264.7 macrophages, indicating the presence of potential anti-inflammatory compounds. Additionally, four anti-inflammatory compounds, including taraxasteryl acetate (1), esculetin (2), pyrimidine-2,4-dione (3), and 5-hydroxypryrolidin-2-one (4), were isolated, and their structures were characterized using 1D and 2D-NMR. This study marked the first report of taraxasteryl acetate, pyrimidine-2,4-dione, and 5-hydroxypryrolidin-2-one being isolated from this species. Furthermore, these compounds exerted their anti-inflammatory role by inhibiting NO production and TNF-α expression. Thus, this study contributes to identifying anti-inflammatory constituents from L. sarmentosa and highlights a potential approach for developing phytotherapeutic agents.https://jurnal.ugm.ac.id/ijc/article/view/103167anti-inflammatoryasteraceaelaunaea sarmentosaisolationraw264.7 macrophages |
| spellingShingle | Thanh Quoc Chau Nguyen Khang Thanh Vo Cua Dinh Duong Toan Phu Huynh Lieu Thi Thuy Ca Giao Huynh Dang Identification of Anti-Inflammatory Components from <i>Launaea sarmentosa</i> Using <i>In Vitro</i> Cell Model Indonesian Journal of Chemistry anti-inflammatory asteraceae launaea sarmentosa isolation raw264.7 macrophages |
| title | Identification of Anti-Inflammatory Components from <i>Launaea sarmentosa</i> Using <i>In Vitro</i> Cell Model |
| title_full | Identification of Anti-Inflammatory Components from <i>Launaea sarmentosa</i> Using <i>In Vitro</i> Cell Model |
| title_fullStr | Identification of Anti-Inflammatory Components from <i>Launaea sarmentosa</i> Using <i>In Vitro</i> Cell Model |
| title_full_unstemmed | Identification of Anti-Inflammatory Components from <i>Launaea sarmentosa</i> Using <i>In Vitro</i> Cell Model |
| title_short | Identification of Anti-Inflammatory Components from <i>Launaea sarmentosa</i> Using <i>In Vitro</i> Cell Model |
| title_sort | identification of anti inflammatory components from i launaea sarmentosa i using i in vitro i cell model |
| topic | anti-inflammatory asteraceae launaea sarmentosa isolation raw264.7 macrophages |
| url | https://jurnal.ugm.ac.id/ijc/article/view/103167 |
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