Confirmation of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin residues in poultry meat and pork via LLE-SPE-GC–MS/MS coupled with derivatization treatment
A gas chromatography-tandem mass technique for simultaneous determination of four fluoroquinolone residues in poultry meat and pork was perfect. Trimethylsilyl diazomethane was applied as the derivatizing agent coupled with liquid–liquid and solid–phase extraction. Full scan mass spectrometry and au...
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Elsevier
2025-04-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525002408 |
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| author | Junjie Xu Yiwei Hong Yun Liu Shuyu Liu Pengfei Gao Yawen Guo Yang Cao Hao Ding Maoda Pang Tao Zhang Genxi Zhang Xiaodong Guo Xing Xie Kaizhou Xie |
| author_facet | Junjie Xu Yiwei Hong Yun Liu Shuyu Liu Pengfei Gao Yawen Guo Yang Cao Hao Ding Maoda Pang Tao Zhang Genxi Zhang Xiaodong Guo Xing Xie Kaizhou Xie |
| author_sort | Junjie Xu |
| collection | DOAJ |
| description | A gas chromatography-tandem mass technique for simultaneous determination of four fluoroquinolone residues in poultry meat and pork was perfect. Trimethylsilyl diazomethane was applied as the derivatizing agent coupled with liquid–liquid and solid–phase extraction. Full scan mass spectrometry and automated selected reaction monitoring were applied for qualitative and quantitative analysis. The limits of detection of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin were 1.0, 0.2, 1.0 and 1.0 μg/kg, respectively, and limits of quantitation were 2.0, 0.4, 2.0 and 2.0 μg/kg, respectively. The range of recoveries were 77.97–92.23 %. The relative standard deviations were less than 7.45 %. All matrices had weak matrix effects. This method conforms to the requirements of Food and Agriculture Organization of United Nations & World Health Organization (2014) for veterinary drug residue testing, expanding the monitoring range and improving the detection efficiency. Only enrofloxacin residues (2.52–18.32 μg/kg) below maximum residue limits were detected in real samples. |
| format | Article |
| id | doaj-art-a620fdc5c89540c68b4a5baf7180d501 |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-a620fdc5c89540c68b4a5baf7180d5012025-08-20T02:30:50ZengElsevierFood Chemistry: X2590-15752025-04-012710239310.1016/j.fochx.2025.102393Confirmation of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin residues in poultry meat and pork via LLE-SPE-GC–MS/MS coupled with derivatization treatmentJunjie Xu0Yiwei Hong1Yun Liu2Shuyu Liu3Pengfei Gao4Yawen Guo5Yang Cao6Hao Ding7Maoda Pang8Tao Zhang9Genxi Zhang10Xiaodong Guo11Xing Xie12Kaizhou Xie13College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, ChinaJiangsu Lushui Qingshan Inspection and Testing Technology Co., LTD, Lianyungang 222000, PR ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, China; College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, China; College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Control Technology and Standard for Agri–product Safety and Quality, Key Laboratory of Food Quality and Safety of Jiangsu Province–State Key Laboratory Breeding Base, Institute of Food Safety and Nutrition, Jiangsu, Academy of Agricultural Sciences, Nanjing 210014, PR ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Veterinary Bio–Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China; Corresponding author at: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri–Product Safety, Yangzhou University, Yangzhou 225009, China; Corresponding author at: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.A gas chromatography-tandem mass technique for simultaneous determination of four fluoroquinolone residues in poultry meat and pork was perfect. Trimethylsilyl diazomethane was applied as the derivatizing agent coupled with liquid–liquid and solid–phase extraction. Full scan mass spectrometry and automated selected reaction monitoring were applied for qualitative and quantitative analysis. The limits of detection of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin were 1.0, 0.2, 1.0 and 1.0 μg/kg, respectively, and limits of quantitation were 2.0, 0.4, 2.0 and 2.0 μg/kg, respectively. The range of recoveries were 77.97–92.23 %. The relative standard deviations were less than 7.45 %. All matrices had weak matrix effects. This method conforms to the requirements of Food and Agriculture Organization of United Nations & World Health Organization (2014) for veterinary drug residue testing, expanding the monitoring range and improving the detection efficiency. Only enrofloxacin residues (2.52–18.32 μg/kg) below maximum residue limits were detected in real samples.http://www.sciencedirect.com/science/article/pii/S2590157525002408DerivatizationFluoroquinolone residuesGas chromatography-tandem massPoultry meat and porkQualitative and quantitative |
| spellingShingle | Junjie Xu Yiwei Hong Yun Liu Shuyu Liu Pengfei Gao Yawen Guo Yang Cao Hao Ding Maoda Pang Tao Zhang Genxi Zhang Xiaodong Guo Xing Xie Kaizhou Xie Confirmation of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin residues in poultry meat and pork via LLE-SPE-GC–MS/MS coupled with derivatization treatment Food Chemistry: X Derivatization Fluoroquinolone residues Gas chromatography-tandem mass Poultry meat and pork Qualitative and quantitative |
| title | Confirmation of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin residues in poultry meat and pork via LLE-SPE-GC–MS/MS coupled with derivatization treatment |
| title_full | Confirmation of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin residues in poultry meat and pork via LLE-SPE-GC–MS/MS coupled with derivatization treatment |
| title_fullStr | Confirmation of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin residues in poultry meat and pork via LLE-SPE-GC–MS/MS coupled with derivatization treatment |
| title_full_unstemmed | Confirmation of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin residues in poultry meat and pork via LLE-SPE-GC–MS/MS coupled with derivatization treatment |
| title_short | Confirmation of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin residues in poultry meat and pork via LLE-SPE-GC–MS/MS coupled with derivatization treatment |
| title_sort | confirmation of enrofloxacin ofloxacin sarafloxacin and danofloxacin residues in poultry meat and pork via lle spe gc ms ms coupled with derivatization treatment |
| topic | Derivatization Fluoroquinolone residues Gas chromatography-tandem mass Poultry meat and pork Qualitative and quantitative |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525002408 |
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