A method for the rapid isolation of virus from cultured cells
A simple and efficient collection method using hypotonic burst to isolate virions from infected cultured cells is described. Distilled water treatment of avian metapneumovirus (AMPV)-infected cells with thorough mixing and repeated pipeting was considerably faster for virion collection in avian cell...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2008-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112589 |
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| _version_ | 1850152416647839744 |
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| author | Byung-Whi Kong Linda K. Foster Douglas N. Foster |
| author_facet | Byung-Whi Kong Linda K. Foster Douglas N. Foster |
| author_sort | Byung-Whi Kong |
| collection | DOAJ |
| description | A simple and efficient collection method using hypotonic burst to isolate virions from infected cultured cells is described. Distilled water treatment of avian metapneumovirus (AMPV)-infected cells with thorough mixing and repeated pipeting was considerably faster for virion collection in avian cells compared to the widely used freeze-thaw (F-T) method (30 min vs. 3–4 h). This method was also more effective for virion collection. The total number of virions recovered from AMPV-infected immortal turkey turbinate cells by the novel water lysis method was 3-fold higher than by the F-T method. This simple water lysis method can be applied to virion collection for other RNA viruses such as the paramyxoviruses that are used to infect cultured cells. |
| format | Article |
| id | doaj-art-a60f7c73d0f14336ba715aaa2f970b75 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-a60f7c73d0f14336ba715aaa2f970b752025-08-20T02:25:59ZengTaylor & Francis GroupBioTechniques0736-62051940-98182008-01-01441979910.2144/000112589A method for the rapid isolation of virus from cultured cellsByung-Whi Kong0Linda K. Foster1Douglas N. Foster21Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR2Department of Animal Science, University of Minnesota, St. Paul, MN, USA2Department of Animal Science, University of Minnesota, St. Paul, MN, USAA simple and efficient collection method using hypotonic burst to isolate virions from infected cultured cells is described. Distilled water treatment of avian metapneumovirus (AMPV)-infected cells with thorough mixing and repeated pipeting was considerably faster for virion collection in avian cells compared to the widely used freeze-thaw (F-T) method (30 min vs. 3–4 h). This method was also more effective for virion collection. The total number of virions recovered from AMPV-infected immortal turkey turbinate cells by the novel water lysis method was 3-fold higher than by the F-T method. This simple water lysis method can be applied to virion collection for other RNA viruses such as the paramyxoviruses that are used to infect cultured cells.https://www.future-science.com/doi/10.2144/000112589 |
| spellingShingle | Byung-Whi Kong Linda K. Foster Douglas N. Foster A method for the rapid isolation of virus from cultured cells BioTechniques |
| title | A method for the rapid isolation of virus from cultured cells |
| title_full | A method for the rapid isolation of virus from cultured cells |
| title_fullStr | A method for the rapid isolation of virus from cultured cells |
| title_full_unstemmed | A method for the rapid isolation of virus from cultured cells |
| title_short | A method for the rapid isolation of virus from cultured cells |
| title_sort | method for the rapid isolation of virus from cultured cells |
| url | https://www.future-science.com/doi/10.2144/000112589 |
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