Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium
The effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe)...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2014/923834 |
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| author | Lérida Liss Flores Villavicencio Gustavo Cruz-Jiménez Gloria Barbosa-Sabanero Carlos Kornhauser-Araujo M. Eugenia Mendoza-Garrido Guadalupe de la Rosa Myrna Sabanero-López |
| author_facet | Lérida Liss Flores Villavicencio Gustavo Cruz-Jiménez Gloria Barbosa-Sabanero Carlos Kornhauser-Araujo M. Eugenia Mendoza-Garrido Guadalupe de la Rosa Myrna Sabanero-López |
| author_sort | Lérida Liss Flores Villavicencio |
| collection | DOAJ |
| description | The effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe) and selenomethionine (SeMet) on cell growth and intracellular structures in lung cancer cells exposed at Se concentrations between 0 and 3 mM. Our results showed that SSe affected cell growth more rapidly than SeMet (24 h and 48 h, resp.). After 24 h of cells exposure to 0.5, 1.5, and 3 mM SSe, cell growth was reduced by 10, 50, and 60%, as compared to controls. After 48 h, nuclear fragmentation was evident in cells exposed to SSe, suggesting an induction to cell death. In contrast, SeMet did not affect cell proliferation, and the cells were phenotypically similar to controls. Microtubules and microfilaments structures were also affected by both Se compounds, again SSe being more toxic than SeMet. To our knowledge, this is the first report on the differential effects of organic and inorganic Se in supranutritional levels in lung cancer cells. |
| format | Article |
| id | doaj-art-a60a68dd99ba43c39b413658aac371e7 |
| institution | OA Journals |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-a60a68dd99ba43c39b413658aac371e72025-08-20T02:20:48ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2014-01-01201410.1155/2014/923834923834Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic SeleniumLérida Liss Flores Villavicencio0Gustavo Cruz-Jiménez1Gloria Barbosa-Sabanero2Carlos Kornhauser-Araujo3M. Eugenia Mendoza-Garrido4Guadalupe de la Rosa5Myrna Sabanero-López6Universidad de Guanajuato, Lascurain de Retana 5, 36000 Guanajuato, GTO, MexicoUniversidad de Guanajuato, Lascurain de Retana 5, 36000 Guanajuato, GTO, MexicoUniversidad de Guanajuato, Lascurain de Retana 5, 36000 Guanajuato, GTO, MexicoUniversidad de Guanajuato, Lascurain de Retana 5, 36000 Guanajuato, GTO, MexicoDepartamento de Fisiología, Biofísica y Neurociencias, CINVESTAV, Avenida Instituto Politécnico Nacional 2508, San Pedro Zacatenco, Gustavo A. Madero, 07360 Ciudad de México, DF, MexicoUniversidad de Guanajuato, Lascurain de Retana 5, 36000 Guanajuato, GTO, MexicoUniversidad de Guanajuato, Lascurain de Retana 5, 36000 Guanajuato, GTO, MexicoThe effects of organic and inorganic forms of selenium (Se) on human cells have been extensively studied for nutritional concentrations; however, to date, little is known about the potential toxicity at supranutritional levels. In the present study we determined the effects of sodium selenite (SSe) and selenomethionine (SeMet) on cell growth and intracellular structures in lung cancer cells exposed at Se concentrations between 0 and 3 mM. Our results showed that SSe affected cell growth more rapidly than SeMet (24 h and 48 h, resp.). After 24 h of cells exposure to 0.5, 1.5, and 3 mM SSe, cell growth was reduced by 10, 50, and 60%, as compared to controls. After 48 h, nuclear fragmentation was evident in cells exposed to SSe, suggesting an induction to cell death. In contrast, SeMet did not affect cell proliferation, and the cells were phenotypically similar to controls. Microtubules and microfilaments structures were also affected by both Se compounds, again SSe being more toxic than SeMet. To our knowledge, this is the first report on the differential effects of organic and inorganic Se in supranutritional levels in lung cancer cells.http://dx.doi.org/10.1155/2014/923834 |
| spellingShingle | Lérida Liss Flores Villavicencio Gustavo Cruz-Jiménez Gloria Barbosa-Sabanero Carlos Kornhauser-Araujo M. Eugenia Mendoza-Garrido Guadalupe de la Rosa Myrna Sabanero-López Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium Bioinorganic Chemistry and Applications |
| title | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
| title_full | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
| title_fullStr | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
| title_full_unstemmed | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
| title_short | Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium |
| title_sort | human lung cancer cell line a 549 atcc is differentially affected by supranutritional organic and inorganic selenium |
| url | http://dx.doi.org/10.1155/2014/923834 |
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