Identification of lncRNAs in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co-expression network analysis
Abstract Background Sepsis-induced immunosuppression involves complex molecular mechanisms, including dysregulated long noncoding RNAs (lncRNAs), which remain poorly understood. Objective We aimed to identify immunosuppression-related lncRNAs and their functional pathways in sepsis. Methods: Using w...
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| Format: | Article |
| Language: | English |
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BMC
2025-04-01
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| Series: | Hereditas |
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| Online Access: | https://doi.org/10.1186/s41065-025-00400-z |
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| author | Wenjia Zhang Yan Li Gang Li Aijia Zhang Wende Sun |
| author_facet | Wenjia Zhang Yan Li Gang Li Aijia Zhang Wende Sun |
| author_sort | Wenjia Zhang |
| collection | DOAJ |
| description | Abstract Background Sepsis-induced immunosuppression involves complex molecular mechanisms, including dysregulated long noncoding RNAs (lncRNAs), which remain poorly understood. Objective We aimed to identify immunosuppression-related lncRNAs and their functional pathways in sepsis. Methods: Using weighted gene coexpression network analysis (WGCNA), we analyzed lncRNA profiles from peripheral blood mononuclear cells (PBMCs) of three sepsis patients and three healthy controls. Key modules linked to immunosuppression were validated via RT-PCR and external datasets. Pathway enrichment and protein interaction networks were employed to prioritize mechanisms. Results A sepsis-associated module containing 4,193 lncRNAs revealed three immunosuppression-related pathways: Th17 cell differentiation, cytokine–cytokine receptor interactions, and cancer-related proteoglycan signaling. Protein–protein interaction networks identified three central genes (SLFN12, ICOS, IKZF2) and their linked lncRNAs (ENSG00000267074, lnc-ICOSLG-1, lnc-IKZF2-7), all significantly downregulated in sepsis patients. Conclusion Our findings highlight novel lncRNA-regulated pathways in sepsis-induced immunosuppression, providing potential targets for improved diagnosis and therapy. |
| format | Article |
| id | doaj-art-a60a17d9701a412da0efb3e3373d2b7a |
| institution | DOAJ |
| issn | 1601-5223 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj-art-a60a17d9701a412da0efb3e3373d2b7a2025-08-20T03:06:54ZengBMCHereditas1601-52232025-04-01162111110.1186/s41065-025-00400-zIdentification of lncRNAs in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co-expression network analysisWenjia Zhang0Yan Li1Gang Li2Aijia Zhang3Wende Sun4Department of Emergency Medicine, China–Japan Friendship HospitalDepartment of Emergency Medicine, China–Japan Friendship HospitalDepartment of Emergency Medicine, China–Japan Friendship HospitalDepartment of Nephrology, Jilin Province People’s HospitalDepartment of Orthopedic and Joint Surgery, Traditional Chinese Medicine Hospital of JuxianAbstract Background Sepsis-induced immunosuppression involves complex molecular mechanisms, including dysregulated long noncoding RNAs (lncRNAs), which remain poorly understood. Objective We aimed to identify immunosuppression-related lncRNAs and their functional pathways in sepsis. Methods: Using weighted gene coexpression network analysis (WGCNA), we analyzed lncRNA profiles from peripheral blood mononuclear cells (PBMCs) of three sepsis patients and three healthy controls. Key modules linked to immunosuppression were validated via RT-PCR and external datasets. Pathway enrichment and protein interaction networks were employed to prioritize mechanisms. Results A sepsis-associated module containing 4,193 lncRNAs revealed three immunosuppression-related pathways: Th17 cell differentiation, cytokine–cytokine receptor interactions, and cancer-related proteoglycan signaling. Protein–protein interaction networks identified three central genes (SLFN12, ICOS, IKZF2) and their linked lncRNAs (ENSG00000267074, lnc-ICOSLG-1, lnc-IKZF2-7), all significantly downregulated in sepsis patients. Conclusion Our findings highlight novel lncRNA-regulated pathways in sepsis-induced immunosuppression, providing potential targets for improved diagnosis and therapy.https://doi.org/10.1186/s41065-025-00400-zSepsisImmunosuppressionlncRNAsWGCNAHub genes |
| spellingShingle | Wenjia Zhang Yan Li Gang Li Aijia Zhang Wende Sun Identification of lncRNAs in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co-expression network analysis Hereditas Sepsis Immunosuppression lncRNAs WGCNA Hub genes |
| title | Identification of lncRNAs in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co-expression network analysis |
| title_full | Identification of lncRNAs in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co-expression network analysis |
| title_fullStr | Identification of lncRNAs in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co-expression network analysis |
| title_full_unstemmed | Identification of lncRNAs in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co-expression network analysis |
| title_short | Identification of lncRNAs in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co-expression network analysis |
| title_sort | identification of lncrnas in peripheral blood mononuclear cells associated with sepsis immunosuppression based on weighted gene co expression network analysis |
| topic | Sepsis Immunosuppression lncRNAs WGCNA Hub genes |
| url | https://doi.org/10.1186/s41065-025-00400-z |
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