Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillus
Nine isolates of Proteus spp. were isolated from 100 urine samples of renal stone patients which were the urine specimens obtained directly from Sulaimani Teaching Hospital Laboratory, and identified according to the cultural characteristic, morphological, biochemical examination. The antibiotic sus...
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| Format: | Article |
| Language: | English |
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Sulaimani Polytechnic University
2018-07-01
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| Series: | Kurdistan Journal of Applied Research |
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| Online Access: | https://www.kjar.spu.edu.iq/index.php/kjar/article/view/205 |
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| author | Srwa Ali Mohammed Mohammed Abdul Aziz Hama Ali Dereh Lattif Mohammed |
| author_facet | Srwa Ali Mohammed Mohammed Abdul Aziz Hama Ali Dereh Lattif Mohammed |
| author_sort | Srwa Ali Mohammed |
| collection | DOAJ |
| description | Nine isolates of Proteus spp. were isolated from 100 urine samples of renal stone patients which were the urine specimens obtained directly from Sulaimani Teaching Hospital Laboratory, and identified according to the cultural characteristic, morphological, biochemical examination. The antibiotic susceptibility test for all isolates were conducted to nine antimicrobial agents including (Ciprofloxacin (Cip), Tetracycline(TE), Neomycin (N), Gentamicin (CN), Erythromycin (E), Nitrofurantoin (F), Naldixic acid (NA), Imipenem (IPM), Amoxicillin (AX). Plasmid analysis of these isolates showed presence are (22) Kb plasmid. Curing of antibiotic resistance genes by using methanol extracts for leave of Crataegus rhipidophylla and Adiantum capillus was performed. The minimum inhibitory concentration of these medicinal plants through methanol extracts which were 5000 µg/ml and 1000 µg/ml for Ailanthus altissima and Adiantum capillus respectively. The Sub minimum inhibition concentration (SMIC) was also determined. The results of transformation and curing experiments revealed that SMIC of Ailanthus altissima extract was cured or eliminated plasmid completely, and (SMIC) of Adiantum capillus was cured (CN, E, and AX) resistant genes.
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| format | Article |
| id | doaj-art-a6059e4ddbb541988d72f78db157f67d |
| institution | Kabale University |
| issn | 2411-7684 2411-7706 |
| language | English |
| publishDate | 2018-07-01 |
| publisher | Sulaimani Polytechnic University |
| record_format | Article |
| series | Kurdistan Journal of Applied Research |
| spelling | doaj-art-a6059e4ddbb541988d72f78db157f67d2025-02-11T21:01:27ZengSulaimani Polytechnic UniversityKurdistan Journal of Applied Research2411-76842411-77062018-07-013210.24017/science.2018.2.17Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillusSrwa Ali Mohammed0Mohammed Abdul Aziz Hama Ali1Dereh Lattif Mohammed2Medical Microbiology Department, Faculty of Science and Health, Koya University, Koya, IraqMedical Microbiology Department, Faculty of Science and Health, Koya University, Koya, IraqPlastic and reconstructive surgery, Faruk Medical city, Private Hospital, Sulaimani, IraqNine isolates of Proteus spp. were isolated from 100 urine samples of renal stone patients which were the urine specimens obtained directly from Sulaimani Teaching Hospital Laboratory, and identified according to the cultural characteristic, morphological, biochemical examination. The antibiotic susceptibility test for all isolates were conducted to nine antimicrobial agents including (Ciprofloxacin (Cip), Tetracycline(TE), Neomycin (N), Gentamicin (CN), Erythromycin (E), Nitrofurantoin (F), Naldixic acid (NA), Imipenem (IPM), Amoxicillin (AX). Plasmid analysis of these isolates showed presence are (22) Kb plasmid. Curing of antibiotic resistance genes by using methanol extracts for leave of Crataegus rhipidophylla and Adiantum capillus was performed. The minimum inhibitory concentration of these medicinal plants through methanol extracts which were 5000 µg/ml and 1000 µg/ml for Ailanthus altissima and Adiantum capillus respectively. The Sub minimum inhibition concentration (SMIC) was also determined. The results of transformation and curing experiments revealed that SMIC of Ailanthus altissima extract was cured or eliminated plasmid completely, and (SMIC) of Adiantum capillus was cured (CN, E, and AX) resistant genes. https://www.kjar.spu.edu.iq/index.php/kjar/article/view/205Proteus sp., Plasmid, antibiotic resistance, transformation, curing, medicinal plants. |
| spellingShingle | Srwa Ali Mohammed Mohammed Abdul Aziz Hama Ali Dereh Lattif Mohammed Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillus Kurdistan Journal of Applied Research Proteus sp., Plasmid, antibiotic resistance, transformation, curing, medicinal plants. |
| title | Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillus |
| title_full | Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillus |
| title_fullStr | Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillus |
| title_full_unstemmed | Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillus |
| title_short | Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillus |
| title_sort | treating antibiotic resistance genes in proteus spp were isolated from renal stone patients by crataegus rhipidophylla and adiantum capillus |
| topic | Proteus sp., Plasmid, antibiotic resistance, transformation, curing, medicinal plants. |
| url | https://www.kjar.spu.edu.iq/index.php/kjar/article/view/205 |
| work_keys_str_mv | AT srwaalimohammed treatingantibioticresistancegenesinproteussppwereisolatedfromrenalstonepatientsbycrataegusrhipidophyllaandadiantumcapillus AT mohammedabdulazizhamaali treatingantibioticresistancegenesinproteussppwereisolatedfromrenalstonepatientsbycrataegusrhipidophyllaandadiantumcapillus AT derehlattifmohammed treatingantibioticresistancegenesinproteussppwereisolatedfromrenalstonepatientsbycrataegusrhipidophyllaandadiantumcapillus |