Growth and Identification of Bacteria in N-Halamine Dental Unit Waterline Tubing Using an Ultrapure Water Source
This study examined bacterial growth and type on biofilm-controlling dental unit waterline (DUWL) tubing (T) and control manufacturer's tubing (C) in a laboratory DUWL model using ultrapure source water that was cycled through the lines. Sections of tubing lines were detached and examined for b...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2011/767314 |
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| _version_ | 1832556350466424832 |
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| author | Nuala Porteous Jie Luo Monica Hererra John Schoolfield Yuyu Sun |
| author_facet | Nuala Porteous Jie Luo Monica Hererra John Schoolfield Yuyu Sun |
| author_sort | Nuala Porteous |
| collection | DOAJ |
| description | This study examined bacterial growth and type on biofilm-controlling dental unit waterline (DUWL) tubing (T) and control manufacturer's tubing (C) in a laboratory DUWL model using ultrapure source water that was cycled through the lines. Sections of tubing lines were detached and examined for biofilm growth using SEM imaging at six sampling periods. Bacteria from inside surfaces of T and C, source unit, and reservoir were cultured and enumerated. At six months, organisms were molecularly identified from the alignment matches obtained from the top three BLAST searches for the 16S region. There was a 1–3 log increase in organism growth in a clean, nonsterile reservoir within an hour. Biofilm was established on the inside surfaces of C within three weeks, but not on T. Proteobacteria, and Sphingomonas spp. were identified in the source reservoir and C line, and a variation of the genera was found in T line. |
| format | Article |
| id | doaj-art-a5f42f89eadf496f85ed849e8c76bce5 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-a5f42f89eadf496f85ed849e8c76bce52025-02-03T05:45:44ZengWileyInternational Journal of Microbiology1687-918X1687-91982011-01-01201110.1155/2011/767314767314Growth and Identification of Bacteria in N-Halamine Dental Unit Waterline Tubing Using an Ultrapure Water SourceNuala Porteous0Jie Luo1Monica Hererra2John Schoolfield3Yuyu Sun4Department of Comprehensive Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USABiomedical Engineering Program, University of South Dakota, Sioux Falls, SD 57107, USADepartment of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USADepartment of Comprehensive Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USABiomedical Engineering Program, University of South Dakota, Sioux Falls, SD 57107, USAThis study examined bacterial growth and type on biofilm-controlling dental unit waterline (DUWL) tubing (T) and control manufacturer's tubing (C) in a laboratory DUWL model using ultrapure source water that was cycled through the lines. Sections of tubing lines were detached and examined for biofilm growth using SEM imaging at six sampling periods. Bacteria from inside surfaces of T and C, source unit, and reservoir were cultured and enumerated. At six months, organisms were molecularly identified from the alignment matches obtained from the top three BLAST searches for the 16S region. There was a 1–3 log increase in organism growth in a clean, nonsterile reservoir within an hour. Biofilm was established on the inside surfaces of C within three weeks, but not on T. Proteobacteria, and Sphingomonas spp. were identified in the source reservoir and C line, and a variation of the genera was found in T line.http://dx.doi.org/10.1155/2011/767314 |
| spellingShingle | Nuala Porteous Jie Luo Monica Hererra John Schoolfield Yuyu Sun Growth and Identification of Bacteria in N-Halamine Dental Unit Waterline Tubing Using an Ultrapure Water Source International Journal of Microbiology |
| title | Growth and Identification of Bacteria in N-Halamine Dental Unit Waterline Tubing Using an Ultrapure Water Source |
| title_full | Growth and Identification of Bacteria in N-Halamine Dental Unit Waterline Tubing Using an Ultrapure Water Source |
| title_fullStr | Growth and Identification of Bacteria in N-Halamine Dental Unit Waterline Tubing Using an Ultrapure Water Source |
| title_full_unstemmed | Growth and Identification of Bacteria in N-Halamine Dental Unit Waterline Tubing Using an Ultrapure Water Source |
| title_short | Growth and Identification of Bacteria in N-Halamine Dental Unit Waterline Tubing Using an Ultrapure Water Source |
| title_sort | growth and identification of bacteria in n halamine dental unit waterline tubing using an ultrapure water source |
| url | http://dx.doi.org/10.1155/2011/767314 |
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