Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation
The NLRP1 (nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-1) inflammasome is the most important inflammasome in human keratinocytes. It plays a crucial role in regulating innate immunity in the skin. This study aimed to evaluate NLRP1 inflammasome activation and t...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/14/11/1427 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850145379700441088 |
|---|---|
| author | Tian Wang Amir S. Yazdi Diana Panayotova-Dimitrova |
| author_facet | Tian Wang Amir S. Yazdi Diana Panayotova-Dimitrova |
| author_sort | Tian Wang |
| collection | DOAJ |
| description | The NLRP1 (nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-1) inflammasome is the most important inflammasome in human keratinocytes. It plays a crucial role in regulating innate immunity in the skin. This study aimed to evaluate NLRP1 inflammasome activation and the corresponding levels of detection in different keratinocyte cell lines to identify a suitable in vitro model for analyzing inflammasome activation in keratinocytes. We compared NLRP1 inflammasome activation, expression, and cell death among primary keratinocytes and immortalized keratinocyte cell lines HaCaT, HaSKpw, and SVTERT upon stimulation with ultraviolet B (UVB) irradiation or talabostat. The effects of both NLRP1 inducers on cell death and the modification of NLRP1 molecules were examined using fluorescence-activated cell sorting analysis, Western blotting, and an enzyme-linked immunosorbent assay. The key inflammasome components had varied expression levels among the keratinocyte cell models, with the highest expression observed in primary keratinocytes. Moreover, our data showed that both UVB and talabostat triggered cell death, and NLRP1 inflammasome activation was readily detected in primary keratinocytes but not in the analyzed immortalized keratinocyte cell lines. Therefore, we do not recommend the use of the immortalized keratinocyte cell lines HaCaT, HaSKpw, and SVTERT for analyzing inflammasome activation in keratinocytes; we strongly recommend the use of primary keratinocytes for these studies. |
| format | Article |
| id | doaj-art-a5eb36de4ff149c8a22648450b50ea62 |
| institution | OA Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-a5eb36de4ff149c8a22648450b50ea622025-08-20T02:28:07ZengMDPI AGBiomolecules2218-273X2024-11-011411142710.3390/biom14111427Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome ActivationTian Wang0Amir S. Yazdi1Diana Panayotova-Dimitrova2Department of Dermatology and Allergology, University Hospital RWTH Aachen, 52074 Aachen, GermanyDepartment of Dermatology and Allergology, University Hospital RWTH Aachen, 52074 Aachen, GermanyDepartment of Dermatology and Allergology, University Hospital RWTH Aachen, 52074 Aachen, GermanyThe NLRP1 (nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-1) inflammasome is the most important inflammasome in human keratinocytes. It plays a crucial role in regulating innate immunity in the skin. This study aimed to evaluate NLRP1 inflammasome activation and the corresponding levels of detection in different keratinocyte cell lines to identify a suitable in vitro model for analyzing inflammasome activation in keratinocytes. We compared NLRP1 inflammasome activation, expression, and cell death among primary keratinocytes and immortalized keratinocyte cell lines HaCaT, HaSKpw, and SVTERT upon stimulation with ultraviolet B (UVB) irradiation or talabostat. The effects of both NLRP1 inducers on cell death and the modification of NLRP1 molecules were examined using fluorescence-activated cell sorting analysis, Western blotting, and an enzyme-linked immunosorbent assay. The key inflammasome components had varied expression levels among the keratinocyte cell models, with the highest expression observed in primary keratinocytes. Moreover, our data showed that both UVB and talabostat triggered cell death, and NLRP1 inflammasome activation was readily detected in primary keratinocytes but not in the analyzed immortalized keratinocyte cell lines. Therefore, we do not recommend the use of the immortalized keratinocyte cell lines HaCaT, HaSKpw, and SVTERT for analyzing inflammasome activation in keratinocytes; we strongly recommend the use of primary keratinocytes for these studies.https://www.mdpi.com/2218-273X/14/11/1427keratinocytesNLRP1 inflammasomeultraviolet Btalabostatcytokines |
| spellingShingle | Tian Wang Amir S. Yazdi Diana Panayotova-Dimitrova Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation Biomolecules keratinocytes NLRP1 inflammasome ultraviolet B talabostat cytokines |
| title | Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation |
| title_full | Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation |
| title_fullStr | Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation |
| title_full_unstemmed | Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation |
| title_short | Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation |
| title_sort | comparison of different keratinocyte cell line models for analysis of nlrp1 inflammasome activation |
| topic | keratinocytes NLRP1 inflammasome ultraviolet B talabostat cytokines |
| url | https://www.mdpi.com/2218-273X/14/11/1427 |
| work_keys_str_mv | AT tianwang comparisonofdifferentkeratinocytecelllinemodelsforanalysisofnlrp1inflammasomeactivation AT amirsyazdi comparisonofdifferentkeratinocytecelllinemodelsforanalysisofnlrp1inflammasomeactivation AT dianapanayotovadimitrova comparisonofdifferentkeratinocytecelllinemodelsforanalysisofnlrp1inflammasomeactivation |