Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.
Cell-cell junctions are an integral part of epithelia and are often disrupted in cancer cells during epithelial-to-mesenchymal transition (EMT), which is a main driver of metastatic spread. We show here that Metastasis suppressor-1 (Mtss1; Missing in Metastasis, MIM), a member of the IMD-family of p...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0031141&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849727541716189184 |
|---|---|
| author | John C Dawson Susann Bruche Heather J Spence Vania M M Braga Laura M Machesky |
| author_facet | John C Dawson Susann Bruche Heather J Spence Vania M M Braga Laura M Machesky |
| author_sort | John C Dawson |
| collection | DOAJ |
| description | Cell-cell junctions are an integral part of epithelia and are often disrupted in cancer cells during epithelial-to-mesenchymal transition (EMT), which is a main driver of metastatic spread. We show here that Metastasis suppressor-1 (Mtss1; Missing in Metastasis, MIM), a member of the IMD-family of proteins, inhibits cell-cell junction disassembly in wound healing or HGF-induced scatter assays by enhancing cell-cell junction strength. Mtss1 not only makes cells more resistant to cell-cell junction disassembly, but also accelerates the kinetics of adherens junction assembly. Mtss1 drives enhanced junction formation specifically by elevating Rac-GTP. Lastly, we show that Mtss1 depletion reduces recruitment of F-actin at cell-cell junctions. We thus propose that Mtss1 promotes Rac1 activation and actin recruitment driving junction maintenance. We suggest that the observed loss of Mtss1 in cancers may compromise junction stability and thus promote EMT and metastasis. |
| format | Article |
| id | doaj-art-a5e57de7038d49d480ef17bb33be33eb |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-a5e57de7038d49d480ef17bb33be33eb2025-08-20T03:09:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3114110.1371/journal.pone.0031141Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.John C DawsonSusann BrucheHeather J SpenceVania M M BragaLaura M MacheskyCell-cell junctions are an integral part of epithelia and are often disrupted in cancer cells during epithelial-to-mesenchymal transition (EMT), which is a main driver of metastatic spread. We show here that Metastasis suppressor-1 (Mtss1; Missing in Metastasis, MIM), a member of the IMD-family of proteins, inhibits cell-cell junction disassembly in wound healing or HGF-induced scatter assays by enhancing cell-cell junction strength. Mtss1 not only makes cells more resistant to cell-cell junction disassembly, but also accelerates the kinetics of adherens junction assembly. Mtss1 drives enhanced junction formation specifically by elevating Rac-GTP. Lastly, we show that Mtss1 depletion reduces recruitment of F-actin at cell-cell junctions. We thus propose that Mtss1 promotes Rac1 activation and actin recruitment driving junction maintenance. We suggest that the observed loss of Mtss1 in cancers may compromise junction stability and thus promote EMT and metastasis.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0031141&type=printable |
| spellingShingle | John C Dawson Susann Bruche Heather J Spence Vania M M Braga Laura M Machesky Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1. PLoS ONE |
| title | Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1. |
| title_full | Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1. |
| title_fullStr | Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1. |
| title_full_unstemmed | Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1. |
| title_short | Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1. |
| title_sort | mtss1 promotes cell cell junction assembly and stability through the small gtpase rac1 |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0031141&type=printable |
| work_keys_str_mv | AT johncdawson mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1 AT susannbruche mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1 AT heatherjspence mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1 AT vaniammbraga mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1 AT laurammachesky mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1 |