Role of antimicrobial peptide on cariogenic biofilm and dental enamel demineralization
Objective: To investigate the effect of antimicrobial peptide (AMP) D1-23 on antibiofilm activity and enamel demineralization by using a microcosm biofilm model. Material and Methods: Human saliva biofilm was grown on bovine enamel discs, which were divided into 4 groups according to the 24-hour gr...
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| Format: | Article |
| Language: | English |
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Universidade Estadual Paulista
2024-11-01
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| Series: | Brazilian Dental Science |
| Online Access: | https://ojs.ict.unesp.br/index.php/cob/article/view/4263 |
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| author | Priscila Vieira da Silva Tatiana Pereira-Cenci Juliana Lays Stolfo Uehara Aurélio Rosa da Silva Júnior Maximiliano Sérgio Cenci Andreza Maria Fábio Aranha |
| author_facet | Priscila Vieira da Silva Tatiana Pereira-Cenci Juliana Lays Stolfo Uehara Aurélio Rosa da Silva Júnior Maximiliano Sérgio Cenci Andreza Maria Fábio Aranha |
| author_sort | Priscila Vieira da Silva |
| collection | DOAJ |
| description |
Objective: To investigate the effect of antimicrobial peptide (AMP) D1-23 on antibiofilm activity and enamel demineralization by using a microcosm biofilm model. Material and Methods: Human saliva biofilm was grown on bovine enamel discs, which were divided into 4 groups according to the 24-hour growth treatment: 0.2 mM AMP D1-23; 1 mM AMP D1-23; Defined Medium with Mucin (DMM; negative control); and 2% chlorhexidine (CHX; positive control). The biomass and number of cultivable cells of a 4-day-old biofilm were evaluated for antibiofilm activity. For analysis of enamel mineral loss by the 7-day-old biofilm and investigated treatments, the Knoop surface microhardness method was performed. ANOVA and Tukey tests were used to analyze data from biomass and enamel surface microhardness. Culturable cells data were analyzed by a pairwise Kruskal-Wallis test. A significance level of 5% was considered for all tests. Results: Although the two concentrations of AMP D1-23 had similar effects on the amount of biofilm biomass (p>0.05), a reduction in biomass was observed when compared to the control group (p < 0.05). The antimicrobial activity of 1 mM AMP D1-23 was similar to that of 2% CHX against Streptococcus mutans, whereas against Streptococci sp and Candida sp it was lower (p <0.05). Also, the 1 mM AMP D1-23 significantly reduced enamel demineralization (p < 0.05). Conclusion: Under the limitations of this study, the 1 mM AMP D1-23 appears to have positive effect on controlling dental enamel demineralization and may be considered a potential treatment for non-cavitated carious lesions.
KEYWORDS
Antimicrobial cationic peptides; Dental caries, Dental plaque; Public health; Tooth remineralization.
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| format | Article |
| id | doaj-art-a5dbb88906794550990bb0059d4f1f0d |
| institution | Kabale University |
| issn | 2178-6011 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Brazilian Dental Science |
| spelling | doaj-art-a5dbb88906794550990bb0059d4f1f0d2025-08-21T14:31:43ZengUniversidade Estadual PaulistaBrazilian Dental Science2178-60112024-11-0127310.4322/bds.2024.e4263Role of antimicrobial peptide on cariogenic biofilm and dental enamel demineralizationPriscila Vieira da Silva0https://orcid.org/0000-0002-6295-2344Tatiana Pereira-Cenci1https://orcid.org/0000-0002-5166-8233Juliana Lays Stolfo Uehara 2https://orcid.org/0000-0002-6387-7892Aurélio Rosa da Silva Júnior 3https://orcid.org/0000-0001-8944-0747Maximiliano Sérgio Cenci4https://orcid.org/0000-0002-2543-6201Andreza Maria Fábio Aranha5https://orcid.org/0000-0003-0014-7937Universidade de Cuiabá-UNIC, Faculdade de Odontologia, Departamento de Ciências Odontológicas Integradas. Cuiabá, MT, Brazil.Universidade Federal de Pelotas, Faculdade de Odontologia. Pelotas, RS, Brazil.Universidade Federal de Pelotas, Faculdade de Odontologia. Pelotas, RS, Brazil.Universidade de Cuiabá-UNIC, Faculdade de Odontologia, Departamento de Ciências Odontológicas Integradas. Cuiabá, MT, Brazil.Universidade Federal de Pelotas, Faculdade de Odontologia. Pelotas, RS, Brazil.Universidade de Cuiabá-UNIC, Faculdade de Odontologia, Departamento de Ciências Odontológicas Integradas. Cuiabá, MT, Brazil. Objective: To investigate the effect of antimicrobial peptide (AMP) D1-23 on antibiofilm activity and enamel demineralization by using a microcosm biofilm model. Material and Methods: Human saliva biofilm was grown on bovine enamel discs, which were divided into 4 groups according to the 24-hour growth treatment: 0.2 mM AMP D1-23; 1 mM AMP D1-23; Defined Medium with Mucin (DMM; negative control); and 2% chlorhexidine (CHX; positive control). The biomass and number of cultivable cells of a 4-day-old biofilm were evaluated for antibiofilm activity. For analysis of enamel mineral loss by the 7-day-old biofilm and investigated treatments, the Knoop surface microhardness method was performed. ANOVA and Tukey tests were used to analyze data from biomass and enamel surface microhardness. Culturable cells data were analyzed by a pairwise Kruskal-Wallis test. A significance level of 5% was considered for all tests. Results: Although the two concentrations of AMP D1-23 had similar effects on the amount of biofilm biomass (p>0.05), a reduction in biomass was observed when compared to the control group (p < 0.05). The antimicrobial activity of 1 mM AMP D1-23 was similar to that of 2% CHX against Streptococcus mutans, whereas against Streptococci sp and Candida sp it was lower (p <0.05). Also, the 1 mM AMP D1-23 significantly reduced enamel demineralization (p < 0.05). Conclusion: Under the limitations of this study, the 1 mM AMP D1-23 appears to have positive effect on controlling dental enamel demineralization and may be considered a potential treatment for non-cavitated carious lesions. KEYWORDS Antimicrobial cationic peptides; Dental caries, Dental plaque; Public health; Tooth remineralization. https://ojs.ict.unesp.br/index.php/cob/article/view/4263 |
| spellingShingle | Priscila Vieira da Silva Tatiana Pereira-Cenci Juliana Lays Stolfo Uehara Aurélio Rosa da Silva Júnior Maximiliano Sérgio Cenci Andreza Maria Fábio Aranha Role of antimicrobial peptide on cariogenic biofilm and dental enamel demineralization Brazilian Dental Science |
| title | Role of antimicrobial peptide on cariogenic biofilm and dental enamel demineralization |
| title_full | Role of antimicrobial peptide on cariogenic biofilm and dental enamel demineralization |
| title_fullStr | Role of antimicrobial peptide on cariogenic biofilm and dental enamel demineralization |
| title_full_unstemmed | Role of antimicrobial peptide on cariogenic biofilm and dental enamel demineralization |
| title_short | Role of antimicrobial peptide on cariogenic biofilm and dental enamel demineralization |
| title_sort | role of antimicrobial peptide on cariogenic biofilm and dental enamel demineralization |
| url | https://ojs.ict.unesp.br/index.php/cob/article/view/4263 |
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