Fibroblast Growth Factor Receptor 3 (FGFR3)–Analyses of the S249C Mutation and Protein Expression in Primary Cervical Carcinomas
Fibroblast growth factor receptor 3 (FGFR3) seems to play an inhibitory role in bone development, as activating mutations in the gene underlie disorders such as achondroplasia and thanatophoric dysplasia. Findings from multiple myeloma (MM) indicate that FGFR3 also can act as an oncogene, and mutati...
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2001/521873 |
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| author | Haiyan Dai Ruth Holm Gunnar B. Kristensen Vera M. Abeler Anne‐Lise Børresen‐Dale Åslaug Helland |
| author_facet | Haiyan Dai Ruth Holm Gunnar B. Kristensen Vera M. Abeler Anne‐Lise Børresen‐Dale Åslaug Helland |
| author_sort | Haiyan Dai |
| collection | DOAJ |
| description | Fibroblast growth factor receptor 3 (FGFR3) seems to play an inhibitory role in bone development, as activating mutations in the gene underlie disorders such as achondroplasia and thanatophoric dysplasia. Findings from multiple myeloma (MM) indicate that FGFR3 also can act as an oncogene, and mutation of codon 249 in the fibroblast growth factor receptor 3 (FGFR3) gene was recently detected in 3/12 primary cervical carcinomas. We have analysed 91 cervical carcinomas for this specific S249C mutation using amplification created restriction site methodology (ACRS), and detected no mutations. Immunohistochemistry was performed on 73 of the tumours. Reduced protein staining was seen in 43 (58.8%) samples. Six of the tumours (8.2%) revealed increased protein staining compared with normal cervical tissue. These patients had a better prognosis than those with reduced or normal levels, although not statistically significant. This report weakens the hypothesis of FGFR3 as an oncogene of importance in cervical carcinomas. |
| format | Article |
| id | doaj-art-a5d93bfb0d814026af5f04f9614b4bd0 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-a5d93bfb0d814026af5f04f9614b4bd02025-02-03T01:03:15ZengWileyAnalytical Cellular Pathology0921-89121878-36512001-01-01232454910.1155/2001/521873Fibroblast Growth Factor Receptor 3 (FGFR3)–Analyses of the S249C Mutation and Protein Expression in Primary Cervical CarcinomasHaiyan Dai0Ruth Holm1Gunnar B. Kristensen2Vera M. Abeler3Anne‐Lise Børresen‐Dale4Åslaug Helland5Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, N‐0310 0slo, NorwayDepartment of Pathology, Institute for Cancer Research, The Norwegian Radium Hospital, N‐0310 0slo, NorwayDepartment of Gynaecological Oncology, Institute for Cancer Research, The Norwegian Radium Hospital, N‐0310 0slo, NorwayDepartment of Pathology, Institute for Cancer Research, The Norwegian Radium Hospital, N‐0310 0slo, NorwayDepartment of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, N‐0310 0slo, NorwayDepartment of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, N‐0310 0slo, NorwayFibroblast growth factor receptor 3 (FGFR3) seems to play an inhibitory role in bone development, as activating mutations in the gene underlie disorders such as achondroplasia and thanatophoric dysplasia. Findings from multiple myeloma (MM) indicate that FGFR3 also can act as an oncogene, and mutation of codon 249 in the fibroblast growth factor receptor 3 (FGFR3) gene was recently detected in 3/12 primary cervical carcinomas. We have analysed 91 cervical carcinomas for this specific S249C mutation using amplification created restriction site methodology (ACRS), and detected no mutations. Immunohistochemistry was performed on 73 of the tumours. Reduced protein staining was seen in 43 (58.8%) samples. Six of the tumours (8.2%) revealed increased protein staining compared with normal cervical tissue. These patients had a better prognosis than those with reduced or normal levels, although not statistically significant. This report weakens the hypothesis of FGFR3 as an oncogene of importance in cervical carcinomas.http://dx.doi.org/10.1155/2001/521873 |
| spellingShingle | Haiyan Dai Ruth Holm Gunnar B. Kristensen Vera M. Abeler Anne‐Lise Børresen‐Dale Åslaug Helland Fibroblast Growth Factor Receptor 3 (FGFR3)–Analyses of the S249C Mutation and Protein Expression in Primary Cervical Carcinomas Analytical Cellular Pathology |
| title | Fibroblast Growth Factor Receptor 3 (FGFR3)–Analyses of the S249C Mutation and Protein Expression in Primary Cervical Carcinomas |
| title_full | Fibroblast Growth Factor Receptor 3 (FGFR3)–Analyses of the S249C Mutation and Protein Expression in Primary Cervical Carcinomas |
| title_fullStr | Fibroblast Growth Factor Receptor 3 (FGFR3)–Analyses of the S249C Mutation and Protein Expression in Primary Cervical Carcinomas |
| title_full_unstemmed | Fibroblast Growth Factor Receptor 3 (FGFR3)–Analyses of the S249C Mutation and Protein Expression in Primary Cervical Carcinomas |
| title_short | Fibroblast Growth Factor Receptor 3 (FGFR3)–Analyses of the S249C Mutation and Protein Expression in Primary Cervical Carcinomas |
| title_sort | fibroblast growth factor receptor 3 fgfr3 analyses of the s249c mutation and protein expression in primary cervical carcinomas |
| url | http://dx.doi.org/10.1155/2001/521873 |
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