Reagent-free Hyperspectral Diagnosis of SARS-CoV-2 Infection in Saliva Samples
Rapid, reagent-free pathogen-agnostic diagnostics that can be performed at the point of need are vital for preparedness against future outbreaks. Yet, many current strategies are pathogen-specific and require several reagents. We present hyperspectral sensing, using light to non-invasively measure t...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2025-01-01
|
| Series: | ECS Sensors Plus |
| Subjects: | |
| Online Access: | https://doi.org/10.1149/2754-2726/ad9c92 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841526589773316096 |
|---|---|
| author | Brandon Saint-John Alejandro Wolf-Yadlin Daniel E. Jacobsen Jamie L. Inman Serge Gart Matt Keener Cynthia McMurray Antoine M. Snijders Harshini Mukundan Jessica Z. Kubicek-Sutherland James B. Brown |
| author_facet | Brandon Saint-John Alejandro Wolf-Yadlin Daniel E. Jacobsen Jamie L. Inman Serge Gart Matt Keener Cynthia McMurray Antoine M. Snijders Harshini Mukundan Jessica Z. Kubicek-Sutherland James B. Brown |
| author_sort | Brandon Saint-John |
| collection | DOAJ |
| description | Rapid, reagent-free pathogen-agnostic diagnostics that can be performed at the point of need are vital for preparedness against future outbreaks. Yet, many current strategies are pathogen-specific and require several reagents. We present hyperspectral sensing, using light to non-invasively measure the composition of several molecules to form a spectral signature, to overcome these barriers. To generate these spectral signatures, we present the ProSpectral ^TM V1, a novel, miniaturized hyperspectral platform with high spectral resolution with two mini-spectrometers. Furthermore, we developed state-of-the-art ML pipelines for near real-time analysis of spectral signatures in saliva samples. We found that we could accurately identify SARS-CoV-2 infection status in double-blinded saliva samples and demonstrate 100% accuracy on a hold out test dataset. To our knowledge, this establishes the fastest hyperspectral diagnostic platform and in a small form factor, and executable with liquid samples, without ligands or reagents, all while maintaining PCR level specificity and sensitivity. |
| format | Article |
| id | doaj-art-a5d4c687151246bc924b1955f494e2cc |
| institution | Kabale University |
| issn | 2754-2726 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | ECS Sensors Plus |
| spelling | doaj-art-a5d4c687151246bc924b1955f494e2cc2025-01-16T17:53:18ZengIOP PublishingECS Sensors Plus2754-27262025-01-014101460110.1149/2754-2726/ad9c92Reagent-free Hyperspectral Diagnosis of SARS-CoV-2 Infection in Saliva SamplesBrandon Saint-John0Alejandro Wolf-Yadlin1Daniel E. Jacobsen2Jamie L. Inman3Serge Gart4Matt Keener5Cynthia McMurray6Antoine M. Snijders7Harshini Mukundan8https://orcid.org/0000-0002-0931-0545Jessica Z. Kubicek-Sutherland9James B. Brown10Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States of AmericaPattern Computer, Inc., Redmond, Washington 98052, United States of AmericaPhysical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States of AmericaBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States of AmericaPattern Computer, Inc., Redmond, Washington 98052, United States of AmericaPattern Computer, Inc., Redmond, Washington 98052, United States of AmericaDivision of Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States of AmericaBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States of AmericaBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States of America; Physical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States of AmericaPhysical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States of AmericaEnvironmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States of America; Department of Statistics, University of California, Berkeley , Berkeley, California 94720, United States of AmericaRapid, reagent-free pathogen-agnostic diagnostics that can be performed at the point of need are vital for preparedness against future outbreaks. Yet, many current strategies are pathogen-specific and require several reagents. We present hyperspectral sensing, using light to non-invasively measure the composition of several molecules to form a spectral signature, to overcome these barriers. To generate these spectral signatures, we present the ProSpectral ^TM V1, a novel, miniaturized hyperspectral platform with high spectral resolution with two mini-spectrometers. Furthermore, we developed state-of-the-art ML pipelines for near real-time analysis of spectral signatures in saliva samples. We found that we could accurately identify SARS-CoV-2 infection status in double-blinded saliva samples and demonstrate 100% accuracy on a hold out test dataset. To our knowledge, this establishes the fastest hyperspectral diagnostic platform and in a small form factor, and executable with liquid samples, without ligands or reagents, all while maintaining PCR level specificity and sensitivity.https://doi.org/10.1149/2754-2726/ad9c92biosensorshyperspectral sensorsagnostic diagnosticscoronavirus |
| spellingShingle | Brandon Saint-John Alejandro Wolf-Yadlin Daniel E. Jacobsen Jamie L. Inman Serge Gart Matt Keener Cynthia McMurray Antoine M. Snijders Harshini Mukundan Jessica Z. Kubicek-Sutherland James B. Brown Reagent-free Hyperspectral Diagnosis of SARS-CoV-2 Infection in Saliva Samples ECS Sensors Plus biosensors hyperspectral sensors agnostic diagnostics coronavirus |
| title | Reagent-free Hyperspectral Diagnosis of SARS-CoV-2 Infection in Saliva Samples |
| title_full | Reagent-free Hyperspectral Diagnosis of SARS-CoV-2 Infection in Saliva Samples |
| title_fullStr | Reagent-free Hyperspectral Diagnosis of SARS-CoV-2 Infection in Saliva Samples |
| title_full_unstemmed | Reagent-free Hyperspectral Diagnosis of SARS-CoV-2 Infection in Saliva Samples |
| title_short | Reagent-free Hyperspectral Diagnosis of SARS-CoV-2 Infection in Saliva Samples |
| title_sort | reagent free hyperspectral diagnosis of sars cov 2 infection in saliva samples |
| topic | biosensors hyperspectral sensors agnostic diagnostics coronavirus |
| url | https://doi.org/10.1149/2754-2726/ad9c92 |
| work_keys_str_mv | AT brandonsaintjohn reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT alejandrowolfyadlin reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT danielejacobsen reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT jamielinman reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT sergegart reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT mattkeener reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT cynthiamcmurray reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT antoinemsnijders reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT harshinimukundan reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT jessicazkubiceksutherland reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples AT jamesbbrown reagentfreehyperspectraldiagnosisofsarscov2infectioninsalivasamples |