Effects of Training Systems on Sugar Accumulation and Metabolism in ‘Kyoho’ Grape Berries
To investigate the effects of straight-line-shape (SL) and inverted-umbrella-shape (IU) training systems on sugar accumulation and metabolism in ‘Kyoho’ grape berries in Fujian, this study used 16-year-old ‘Kyoho’ grapevines trained in the two systems. Fruit samples were collected from 45 to 95 days...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/6/578 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849433242486177792 |
|---|---|
| author | Xinming Liu Jinhui Lin Ting Chen Keyang Chen Yan Lei |
| author_facet | Xinming Liu Jinhui Lin Ting Chen Keyang Chen Yan Lei |
| author_sort | Xinming Liu |
| collection | DOAJ |
| description | To investigate the effects of straight-line-shape (SL) and inverted-umbrella-shape (IU) training systems on sugar accumulation and metabolism in ‘Kyoho’ grape berries in Fujian, this study used 16-year-old ‘Kyoho’ grapevines trained in the two systems. Fruit samples were collected from 45 to 95 days after flowering (DAF) to measure soluble sugar content. Transcriptome sequencing was performed to analyze the differential expression of sugar metabolism-related genes, combined with KEGG enrichment analysis and RT-qPCR validation of key genes. The results showed that, at the same stage, the soluble sugar content in berries under the SL training system was significantly higher than that under the IU training system, especially from 45 to 65 DAF, where sugar accumulation was faster. Transcriptome analysis revealed that the SL training system showed 6274, 5597, and 2064 differentially expressed genes at 45, 65, and 95 DAF, respectively. Key sugar metabolism-related genes, such as fructokinase (<i>FK</i>), phosphofructokinase (<i>PFK</i>), and sucrose phosphate synthase (<i>SPS</i>), exhibited significantly higher expression levels in the SL training system than in the IU training system. KEGG enrichment analysis indicated that the SL training system significantly enriched sugar metabolism and transport pathways during the early fruit ripening stage. RT-qPCR validation confirmed that genes related to sugar metabolism and transport (such as <i>FK7</i>, <i>SUS3</i>, <i>SPP1</i>) were expressed at significantly higher levels in the SL training system than in the IU training system. In conclusion, the SL training system significantly promoted soluble sugar accumulation and accelerated fruit ripening in ‘Kyoho’ grapes by regulating the expression of sugar metabolism and transport-related genes, providing a theoretical basis for promoting the SL training system in production. |
| format | Article |
| id | doaj-art-a5d4a917454146efa9669b7e72f87ce0 |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-a5d4a917454146efa9669b7e72f87ce02025-08-20T03:27:07ZengMDPI AGHorticulturae2311-75242025-05-0111657810.3390/horticulturae11060578Effects of Training Systems on Sugar Accumulation and Metabolism in ‘Kyoho’ Grape BerriesXinming Liu0Jinhui Lin1Ting Chen2Keyang Chen3Yan Lei4Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFAFU-Dal Joint College, Fujian Agriculture and Forestry University, Landscape Architecture (China-Canada Cooperation), Truro, NS B2N 5E3, CanadaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaTo investigate the effects of straight-line-shape (SL) and inverted-umbrella-shape (IU) training systems on sugar accumulation and metabolism in ‘Kyoho’ grape berries in Fujian, this study used 16-year-old ‘Kyoho’ grapevines trained in the two systems. Fruit samples were collected from 45 to 95 days after flowering (DAF) to measure soluble sugar content. Transcriptome sequencing was performed to analyze the differential expression of sugar metabolism-related genes, combined with KEGG enrichment analysis and RT-qPCR validation of key genes. The results showed that, at the same stage, the soluble sugar content in berries under the SL training system was significantly higher than that under the IU training system, especially from 45 to 65 DAF, where sugar accumulation was faster. Transcriptome analysis revealed that the SL training system showed 6274, 5597, and 2064 differentially expressed genes at 45, 65, and 95 DAF, respectively. Key sugar metabolism-related genes, such as fructokinase (<i>FK</i>), phosphofructokinase (<i>PFK</i>), and sucrose phosphate synthase (<i>SPS</i>), exhibited significantly higher expression levels in the SL training system than in the IU training system. KEGG enrichment analysis indicated that the SL training system significantly enriched sugar metabolism and transport pathways during the early fruit ripening stage. RT-qPCR validation confirmed that genes related to sugar metabolism and transport (such as <i>FK7</i>, <i>SUS3</i>, <i>SPP1</i>) were expressed at significantly higher levels in the SL training system than in the IU training system. In conclusion, the SL training system significantly promoted soluble sugar accumulation and accelerated fruit ripening in ‘Kyoho’ grapes by regulating the expression of sugar metabolism and transport-related genes, providing a theoretical basis for promoting the SL training system in production.https://www.mdpi.com/2311-7524/11/6/578<i>Vitis vinifera</i>training system modificationsugar metabolismtranscriptomedifferentially expressed genes |
| spellingShingle | Xinming Liu Jinhui Lin Ting Chen Keyang Chen Yan Lei Effects of Training Systems on Sugar Accumulation and Metabolism in ‘Kyoho’ Grape Berries Horticulturae <i>Vitis vinifera</i> training system modification sugar metabolism transcriptome differentially expressed genes |
| title | Effects of Training Systems on Sugar Accumulation and Metabolism in ‘Kyoho’ Grape Berries |
| title_full | Effects of Training Systems on Sugar Accumulation and Metabolism in ‘Kyoho’ Grape Berries |
| title_fullStr | Effects of Training Systems on Sugar Accumulation and Metabolism in ‘Kyoho’ Grape Berries |
| title_full_unstemmed | Effects of Training Systems on Sugar Accumulation and Metabolism in ‘Kyoho’ Grape Berries |
| title_short | Effects of Training Systems on Sugar Accumulation and Metabolism in ‘Kyoho’ Grape Berries |
| title_sort | effects of training systems on sugar accumulation and metabolism in kyoho grape berries |
| topic | <i>Vitis vinifera</i> training system modification sugar metabolism transcriptome differentially expressed genes |
| url | https://www.mdpi.com/2311-7524/11/6/578 |
| work_keys_str_mv | AT xinmingliu effectsoftrainingsystemsonsugaraccumulationandmetabolisminkyohograpeberries AT jinhuilin effectsoftrainingsystemsonsugaraccumulationandmetabolisminkyohograpeberries AT tingchen effectsoftrainingsystemsonsugaraccumulationandmetabolisminkyohograpeberries AT keyangchen effectsoftrainingsystemsonsugaraccumulationandmetabolisminkyohograpeberries AT yanlei effectsoftrainingsystemsonsugaraccumulationandmetabolisminkyohograpeberries |