Pyrroloquinoline quinone promotes porcine oocyte maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial function
Objective The present study evaluated the beneficial effects of pyrroloquinoline quinone (PQQ) on in vitro maturation (IVM) of porcine oocyte and subsequent early embryo development. Methods Porcine cumulus oocyte complexes were cultured in IVM medium with supplementation of 0, 200, 400, 800 or 1600...
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Asian-Australasian Association of Animal Production Societies
2025-08-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0847.pdf |
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| author | Zehua Zhang Zhigang Gao Zhenwei Jia |
| author_facet | Zehua Zhang Zhigang Gao Zhenwei Jia |
| author_sort | Zehua Zhang |
| collection | DOAJ |
| description | Objective The present study evaluated the beneficial effects of pyrroloquinoline quinone (PQQ) on in vitro maturation (IVM) of porcine oocyte and subsequent early embryo development. Methods Porcine cumulus oocyte complexes were cultured in IVM medium with supplementation of 0, 200, 400, 800 or 1600 nM PQQ for 42 h. We first examined cumulus expansion index (CEI) and the rate of oocyte nuclear maturation. Then, we assessed oocyte mitochondrial function, oxidative stress levels, lipid metabolism and subsequent embryonic development. Results PQQ (800 nM) supplementation significantly increased CEI and the nuclear maturation rate of oocytes following IVM. Additionally, oocysts supplemented with 800 nM of PQQ showed significantly increased mitochondrial content, mitochondrial membrane potential, activity, and mRNA expression levels of genes associated with mitochondrial biogenesis (PGC-1a, NRF1, NRF2 and TFAM). PQQ significantly reduced the levels of reactive oxygen species, lipid drop-lets, and fatty acids content, while enhancing the mRNA expression levels of genes related to antioxidant activity (SOD1, SOD2, GPX and CAT), lipolysis (ATGL and HSL) and β-oxidation (CPT1B and CPT2) in porcine oocytes. PQQ (800 nM) supplementation significantly increased cleavage rate, blastocyst formation rate, and total blastocyst cell numbers following partheno-genetic activation. Conclusion PQQ supplementation during IVM positively influences porcine oocyte maturation and subse-quent embryonic development by enhancing mitochondrial function and lipid metabolism and alleviating oxidative stress. |
| format | Article |
| id | doaj-art-a5d1ac8f4cb64f2d9132fd8c00075c6e |
| institution | Kabale University |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-a5d1ac8f4cb64f2d9132fd8c00075c6e2025-08-20T03:32:51ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-08-013881644165610.5713/ab.24.084725466Pyrroloquinoline quinone promotes porcine oocyte maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial functionZehua Zhang0Zhigang Gao1Zhenwei Jia2 College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, ChinaObjective The present study evaluated the beneficial effects of pyrroloquinoline quinone (PQQ) on in vitro maturation (IVM) of porcine oocyte and subsequent early embryo development. Methods Porcine cumulus oocyte complexes were cultured in IVM medium with supplementation of 0, 200, 400, 800 or 1600 nM PQQ for 42 h. We first examined cumulus expansion index (CEI) and the rate of oocyte nuclear maturation. Then, we assessed oocyte mitochondrial function, oxidative stress levels, lipid metabolism and subsequent embryonic development. Results PQQ (800 nM) supplementation significantly increased CEI and the nuclear maturation rate of oocytes following IVM. Additionally, oocysts supplemented with 800 nM of PQQ showed significantly increased mitochondrial content, mitochondrial membrane potential, activity, and mRNA expression levels of genes associated with mitochondrial biogenesis (PGC-1a, NRF1, NRF2 and TFAM). PQQ significantly reduced the levels of reactive oxygen species, lipid drop-lets, and fatty acids content, while enhancing the mRNA expression levels of genes related to antioxidant activity (SOD1, SOD2, GPX and CAT), lipolysis (ATGL and HSL) and β-oxidation (CPT1B and CPT2) in porcine oocytes. PQQ (800 nM) supplementation significantly increased cleavage rate, blastocyst formation rate, and total blastocyst cell numbers following partheno-genetic activation. Conclusion PQQ supplementation during IVM positively influences porcine oocyte maturation and subse-quent embryonic development by enhancing mitochondrial function and lipid metabolism and alleviating oxidative stress.http://www.animbiosci.org/upload/pdf/ab-24-0847.pdfembryo developmentlipid metabolismmitochondria functionoocyte maturationpyrroloquinoline quinone |
| spellingShingle | Zehua Zhang Zhigang Gao Zhenwei Jia Pyrroloquinoline quinone promotes porcine oocyte maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial function Animal Bioscience embryo development lipid metabolism mitochondria function oocyte maturation pyrroloquinoline quinone |
| title | Pyrroloquinoline quinone promotes porcine oocyte maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial function |
| title_full | Pyrroloquinoline quinone promotes porcine oocyte maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial function |
| title_fullStr | Pyrroloquinoline quinone promotes porcine oocyte maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial function |
| title_full_unstemmed | Pyrroloquinoline quinone promotes porcine oocyte maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial function |
| title_short | Pyrroloquinoline quinone promotes porcine oocyte maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial function |
| title_sort | pyrroloquinoline quinone promotes porcine oocyte maturation and subsequent embryo development by enhancing lipid metabolism and improving mitochondrial function |
| topic | embryo development lipid metabolism mitochondria function oocyte maturation pyrroloquinoline quinone |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0847.pdf |
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