Aerobic exercise and DNA methylation in postmenopausal women: An ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial.
Physical activity is associated with a lower risk of breast, colon, and endometrial cancer. Epigenetic mechanisms such as changes in DNA methylation may help to explain these protective effects. We assessed the impact of a one year aerobic exercise intervention on DNA methylation biomarkers believed...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0198641&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850076742135316480 |
|---|---|
| author | Devon J Boyne Will D King Darren R Brenner John B McIntyre Kerry S Courneya Christine M Friedenreich |
| author_facet | Devon J Boyne Will D King Darren R Brenner John B McIntyre Kerry S Courneya Christine M Friedenreich |
| author_sort | Devon J Boyne |
| collection | DOAJ |
| description | Physical activity is associated with a lower risk of breast, colon, and endometrial cancer. Epigenetic mechanisms such as changes in DNA methylation may help to explain these protective effects. We assessed the impact of a one year aerobic exercise intervention on DNA methylation biomarkers believed to play a role in carcinogenesis. The Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial was a two-armed randomized controlled trial in 320 healthy, inactive, postmenopausal women with no history of cancer. In an ancillary analysis, frozen blood samples (n = 256) were reassessed for levels of DNA methylation within LINE-1 and Alu repeats as well as within the promoter regions of APC, BRCA1, RASSF1, and hTERT genes. Differences between the exercise and control arm at 12-months, after adjusting for baseline values, were estimated within an intent-to-treat and per-protocol analysis using linear regression. No significant differences in DNA methylation between the exercise and control arms were observed. In an exploratory analysis, we found that the prospective change in estimated VO2max was negatively associated with RASSF1 methylation in a dose-response manner (p-trend = 0.04). A year-long aerobic exercise intervention does not affect LINE-1, Alu, APC, BRCA1, RASSF1, or hTERT methylation in healthy, inactive, postmenopausal women. Changes in DNA methylation within these genomic regions may not mediate the association between physical activity and cancer in healthy postmenopausal women. Additional research is needed to validate our findings with RASSF1 methylation.<h4>Trial registration</h4>ClinicalTrials.gov NCT00522262. |
| format | Article |
| id | doaj-art-a5d17a5c19a14de09f32a4929f12ac2f |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-a5d17a5c19a14de09f32a4929f12ac2f2025-08-20T02:45:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019864110.1371/journal.pone.0198641Aerobic exercise and DNA methylation in postmenopausal women: An ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial.Devon J BoyneWill D KingDarren R BrennerJohn B McIntyreKerry S CourneyaChristine M FriedenreichPhysical activity is associated with a lower risk of breast, colon, and endometrial cancer. Epigenetic mechanisms such as changes in DNA methylation may help to explain these protective effects. We assessed the impact of a one year aerobic exercise intervention on DNA methylation biomarkers believed to play a role in carcinogenesis. The Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial was a two-armed randomized controlled trial in 320 healthy, inactive, postmenopausal women with no history of cancer. In an ancillary analysis, frozen blood samples (n = 256) were reassessed for levels of DNA methylation within LINE-1 and Alu repeats as well as within the promoter regions of APC, BRCA1, RASSF1, and hTERT genes. Differences between the exercise and control arm at 12-months, after adjusting for baseline values, were estimated within an intent-to-treat and per-protocol analysis using linear regression. No significant differences in DNA methylation between the exercise and control arms were observed. In an exploratory analysis, we found that the prospective change in estimated VO2max was negatively associated with RASSF1 methylation in a dose-response manner (p-trend = 0.04). A year-long aerobic exercise intervention does not affect LINE-1, Alu, APC, BRCA1, RASSF1, or hTERT methylation in healthy, inactive, postmenopausal women. Changes in DNA methylation within these genomic regions may not mediate the association between physical activity and cancer in healthy postmenopausal women. Additional research is needed to validate our findings with RASSF1 methylation.<h4>Trial registration</h4>ClinicalTrials.gov NCT00522262.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0198641&type=printable |
| spellingShingle | Devon J Boyne Will D King Darren R Brenner John B McIntyre Kerry S Courneya Christine M Friedenreich Aerobic exercise and DNA methylation in postmenopausal women: An ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial. PLoS ONE |
| title | Aerobic exercise and DNA methylation in postmenopausal women: An ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial. |
| title_full | Aerobic exercise and DNA methylation in postmenopausal women: An ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial. |
| title_fullStr | Aerobic exercise and DNA methylation in postmenopausal women: An ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial. |
| title_full_unstemmed | Aerobic exercise and DNA methylation in postmenopausal women: An ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial. |
| title_short | Aerobic exercise and DNA methylation in postmenopausal women: An ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial. |
| title_sort | aerobic exercise and dna methylation in postmenopausal women an ancillary analysis of the alberta physical activity and breast cancer prevention alpha trial |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0198641&type=printable |
| work_keys_str_mv | AT devonjboyne aerobicexerciseanddnamethylationinpostmenopausalwomenanancillaryanalysisofthealbertaphysicalactivityandbreastcancerpreventionalphatrial AT willdking aerobicexerciseanddnamethylationinpostmenopausalwomenanancillaryanalysisofthealbertaphysicalactivityandbreastcancerpreventionalphatrial AT darrenrbrenner aerobicexerciseanddnamethylationinpostmenopausalwomenanancillaryanalysisofthealbertaphysicalactivityandbreastcancerpreventionalphatrial AT johnbmcintyre aerobicexerciseanddnamethylationinpostmenopausalwomenanancillaryanalysisofthealbertaphysicalactivityandbreastcancerpreventionalphatrial AT kerryscourneya aerobicexerciseanddnamethylationinpostmenopausalwomenanancillaryanalysisofthealbertaphysicalactivityandbreastcancerpreventionalphatrial AT christinemfriedenreich aerobicexerciseanddnamethylationinpostmenopausalwomenanancillaryanalysisofthealbertaphysicalactivityandbreastcancerpreventionalphatrial |