Effects of inter-individual variability in experimenters’ sensitivity and training on behavioral assessment of animal models of vestibular disorders
BackgroundThis study was designed to explore the correlation between animal behavioral assessment quality and rater’s individual sensitivity and training.MethodsWe selected different raters to form a panel to rate the severity of posturo-locomotor deficits in animals displaying excitotoxic or ototox...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2025.1532927/full |
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| author | Romain Boularand Bérénice Hatat Claire Bringuier Nicolas Chanut Abdessadek El Ahmadi Stéphane Besnard Stéphane Besnard Brahim Tighilet Brahim Tighilet Brahim Tighilet Christian Chabbert Christian Chabbert Christian Chabbert |
| author_facet | Romain Boularand Bérénice Hatat Claire Bringuier Nicolas Chanut Abdessadek El Ahmadi Stéphane Besnard Stéphane Besnard Brahim Tighilet Brahim Tighilet Brahim Tighilet Christian Chabbert Christian Chabbert Christian Chabbert |
| author_sort | Romain Boularand |
| collection | DOAJ |
| description | BackgroundThis study was designed to explore the correlation between animal behavioral assessment quality and rater’s individual sensitivity and training.MethodsWe selected different raters to form a panel to rate the severity of posturo-locomotor deficits in animals displaying excitotoxic or ototoxic lesions-induced vestibular syndrome. All raters, regardless of their scientific level, received brief training based on videos and tutorial files. They then had to score videos of rats with different types and stages of vestibular syndromes. All data were collected and analyzed.ResultsInter-individual variability in raters significantly altered the results of behavioral assessment of posturo-locomotor deficits in vestibulo-lesioned animals. Neither gender nor scientific level had an impact on the results. In contrast, the sensitivity of the individual to animal welfare impacted the mean score in the ototoxic lesion model. Raters with high sensitivity tended to exaggerate the symptomatology.ConclusionThe use of automated assessments of posturo-locomotor deficits in vestibulo-lesioned rodents, is the best solution to limit these assessment biases. |
| format | Article |
| id | doaj-art-a5ca7b75e93e421b85d274a3b7088a42 |
| institution | DOAJ |
| issn | 1664-2295 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neurology |
| spelling | doaj-art-a5ca7b75e93e421b85d274a3b7088a422025-08-20T02:54:29ZengFrontiers Media S.A.Frontiers in Neurology1664-22952025-03-011610.3389/fneur.2025.15329271532927Effects of inter-individual variability in experimenters’ sensitivity and training on behavioral assessment of animal models of vestibular disordersRomain Boularand0Bérénice Hatat1Claire Bringuier2Nicolas Chanut3Abdessadek El Ahmadi4Stéphane Besnard5Stéphane Besnard6Brahim Tighilet7Brahim Tighilet8Brahim Tighilet9Christian Chabbert10Christian Chabbert11Christian Chabbert12Vertidiag, Montpellier, FranceVertidiag, Montpellier, FranceVertidiag, Montpellier, FranceVertidiag, Montpellier, FranceCentre de Recherche en Psychologie et Neurosciences, Aix Marseille Université-CNRS UMR 7077, Marseille, FranceUnité GDR 2074 CNRS, Marseille, FranceUR Vertex 7480, Université de Caen Normandie, CHU Caen, Caen, FranceVertidiag, Montpellier, FranceCentre de Recherche en Psychologie et Neurosciences, Aix Marseille Université-CNRS UMR 7077, Marseille, FranceUnité GDR 2074 CNRS, Marseille, FranceVertidiag, Montpellier, FranceCentre de Recherche en Psychologie et Neurosciences, Aix Marseille Université-CNRS UMR 7077, Marseille, FranceUnité GDR 2074 CNRS, Marseille, FranceBackgroundThis study was designed to explore the correlation between animal behavioral assessment quality and rater’s individual sensitivity and training.MethodsWe selected different raters to form a panel to rate the severity of posturo-locomotor deficits in animals displaying excitotoxic or ototoxic lesions-induced vestibular syndrome. All raters, regardless of their scientific level, received brief training based on videos and tutorial files. They then had to score videos of rats with different types and stages of vestibular syndromes. All data were collected and analyzed.ResultsInter-individual variability in raters significantly altered the results of behavioral assessment of posturo-locomotor deficits in vestibulo-lesioned animals. Neither gender nor scientific level had an impact on the results. In contrast, the sensitivity of the individual to animal welfare impacted the mean score in the ototoxic lesion model. Raters with high sensitivity tended to exaggerate the symptomatology.ConclusionThe use of automated assessments of posturo-locomotor deficits in vestibulo-lesioned rodents, is the best solution to limit these assessment biases.https://www.frontiersin.org/articles/10.3389/fneur.2025.1532927/fullanimal experimentationrisk of biasmethodsvestibular pathologiesinter-individual variabilitypathological models |
| spellingShingle | Romain Boularand Bérénice Hatat Claire Bringuier Nicolas Chanut Abdessadek El Ahmadi Stéphane Besnard Stéphane Besnard Brahim Tighilet Brahim Tighilet Brahim Tighilet Christian Chabbert Christian Chabbert Christian Chabbert Effects of inter-individual variability in experimenters’ sensitivity and training on behavioral assessment of animal models of vestibular disorders Frontiers in Neurology animal experimentation risk of bias methods vestibular pathologies inter-individual variability pathological models |
| title | Effects of inter-individual variability in experimenters’ sensitivity and training on behavioral assessment of animal models of vestibular disorders |
| title_full | Effects of inter-individual variability in experimenters’ sensitivity and training on behavioral assessment of animal models of vestibular disorders |
| title_fullStr | Effects of inter-individual variability in experimenters’ sensitivity and training on behavioral assessment of animal models of vestibular disorders |
| title_full_unstemmed | Effects of inter-individual variability in experimenters’ sensitivity and training on behavioral assessment of animal models of vestibular disorders |
| title_short | Effects of inter-individual variability in experimenters’ sensitivity and training on behavioral assessment of animal models of vestibular disorders |
| title_sort | effects of inter individual variability in experimenters sensitivity and training on behavioral assessment of animal models of vestibular disorders |
| topic | animal experimentation risk of bias methods vestibular pathologies inter-individual variability pathological models |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2025.1532927/full |
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