Detection of dicistroviruses RNA in blood of febrile Tanzanian children
Fever is the leading cause of paediatric outpatient consultations in Sub-Saharan Africa. Although most are suspected to be of viral origin, a putative causative pathogen is not identified in over a quarter of these febrile episodes. Using a de novo assembly sequencing approach, we report the detecti...
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Taylor & Francis Group
2019-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1603791 |
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| author | Samuel Cordey Florian Laubscher Mary-Anne Hartley Thomas Junier Francisco J. Pérez-Rodriguez Kristina Keitel Gael Vieille Josephine Samaka Tarsis Mlaganile Frank Kagoro Noémie Boillat-Blanco Zainab Mbarack Mylène Docquier Francisco Brito Daniel Eibach Jürgen May Peter Sothmann Cassandra Aldrich John Lusingu Caroline Tapparel Valérie D’Acremont Laurent Kaiser |
| author_facet | Samuel Cordey Florian Laubscher Mary-Anne Hartley Thomas Junier Francisco J. Pérez-Rodriguez Kristina Keitel Gael Vieille Josephine Samaka Tarsis Mlaganile Frank Kagoro Noémie Boillat-Blanco Zainab Mbarack Mylène Docquier Francisco Brito Daniel Eibach Jürgen May Peter Sothmann Cassandra Aldrich John Lusingu Caroline Tapparel Valérie D’Acremont Laurent Kaiser |
| author_sort | Samuel Cordey |
| collection | DOAJ |
| description | Fever is the leading cause of paediatric outpatient consultations in Sub-Saharan Africa. Although most are suspected to be of viral origin, a putative causative pathogen is not identified in over a quarter of these febrile episodes. Using a de novo assembly sequencing approach, we report the detection (15.4%) of dicistroviruses (DicV) RNA in sera collected from 692 febrile Tanzanian children. In contrast, DicV RNA was only detected in 1/77 (1.3%) plasma samples from febrile Tanzanian adults, suggesting that children could represent the primary susceptible population. Estimated viral load by specific quantitative real-time RT–PCR assay ranged from < 1.32E3 to 1.44E7 viral RNA copies/mL serum. Three DicV full-length genomes were obtained, and a phylogenetic analyse on the capsid region showed the presence of two clusters representing tentative novel genus. Although DicV-positive cases were detected throughout the year, a significantly higher positivity rate was observed during the rainy season.This study reveals that novel DicV RNA is frequently detected in the blood of Tanzanian children, paving the way for further investigations to determine if DicV possibly represent a new agent in humans. |
| format | Article |
| id | doaj-art-a5be99b5fe8f4a26a02b3af3ed43f439 |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-a5be99b5fe8f4a26a02b3af3ed43f4392025-08-20T03:17:55ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-018161362310.1080/22221751.2019.1603791Detection of dicistroviruses RNA in blood of febrile Tanzanian childrenSamuel Cordey0Florian Laubscher1Mary-Anne Hartley2Thomas Junier3Francisco J. Pérez-Rodriguez4Kristina Keitel5Gael Vieille6Josephine Samaka7Tarsis Mlaganile8Frank Kagoro9Noémie Boillat-Blanco10Zainab Mbarack11Mylène Docquier12Francisco Brito13Daniel Eibach14Jürgen May15Peter Sothmann16Cassandra Aldrich17John Lusingu18Caroline Tapparel19Valérie D’Acremont20Laurent Kaiser21Division of Infectious Diseases and Laboratory of Virology, University of Geneva Hospitals Geneva, SwitzerlandDivision of Infectious Diseases and Laboratory of Virology, University of Geneva Hospitals Geneva, SwitzerlandDepartment of Ambulatory Care and Community Medicine, Lausanne University Hospital Lausanne, SwitzerlandSwiss Institute of Bioinformatics Geneva, SwitzerlandUniversity of Geneva Medical School Geneva, SwitzerlandSwiss Tropical and Public Health Institute, University of Basel Basel, SwitzerlandDivision of Infectious Diseases and Laboratory of Virology, University of Geneva Hospitals Geneva, SwitzerlandIfakara Health Institute, Dar es Salaam, TanzaniaIfakara Health Institute, Dar es Salaam, TanzaniaIfakara Health Institute, Dar es Salaam, TanzaniaSwiss Tropical and Public Health Institute, University of Basel Basel, SwitzerlandMwananyamala Hospital, Dar es Salaam, TanzaniaiGE3 Genomics Platform, University of Geneva Geneva, SwitzerlandSwiss Institute of Bioinformatics Geneva, SwitzerlandDepartment of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine Hamburg, GermanyDepartment of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine Hamburg, GermanyDepartment of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine Hamburg, GermanyDepartment of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine Hamburg, GermanyNational Institute for Medical Research, Tanga Research Centre, Tanga, TanzaniaUniversity of Geneva Medical School Geneva, SwitzerlandDepartment of Ambulatory Care and Community Medicine, Lausanne University Hospital Lausanne, SwitzerlandDivision of Infectious Diseases and Laboratory of Virology, University of Geneva Hospitals Geneva, SwitzerlandFever is the leading cause of paediatric outpatient consultations in Sub-Saharan Africa. Although most are suspected to be of viral origin, a putative causative pathogen is not identified in over a quarter of these febrile episodes. Using a de novo assembly sequencing approach, we report the detection (15.4%) of dicistroviruses (DicV) RNA in sera collected from 692 febrile Tanzanian children. In contrast, DicV RNA was only detected in 1/77 (1.3%) plasma samples from febrile Tanzanian adults, suggesting that children could represent the primary susceptible population. Estimated viral load by specific quantitative real-time RT–PCR assay ranged from < 1.32E3 to 1.44E7 viral RNA copies/mL serum. Three DicV full-length genomes were obtained, and a phylogenetic analyse on the capsid region showed the presence of two clusters representing tentative novel genus. Although DicV-positive cases were detected throughout the year, a significantly higher positivity rate was observed during the rainy season.This study reveals that novel DicV RNA is frequently detected in the blood of Tanzanian children, paving the way for further investigations to determine if DicV possibly represent a new agent in humans.https://www.tandfonline.com/doi/10.1080/22221751.2019.1603791Dicistrovirusde novo analysisseraviremiaTanzanian children |
| spellingShingle | Samuel Cordey Florian Laubscher Mary-Anne Hartley Thomas Junier Francisco J. Pérez-Rodriguez Kristina Keitel Gael Vieille Josephine Samaka Tarsis Mlaganile Frank Kagoro Noémie Boillat-Blanco Zainab Mbarack Mylène Docquier Francisco Brito Daniel Eibach Jürgen May Peter Sothmann Cassandra Aldrich John Lusingu Caroline Tapparel Valérie D’Acremont Laurent Kaiser Detection of dicistroviruses RNA in blood of febrile Tanzanian children Emerging Microbes and Infections Dicistrovirus de novo analysis sera viremia Tanzanian children |
| title | Detection of dicistroviruses RNA in blood of febrile Tanzanian children |
| title_full | Detection of dicistroviruses RNA in blood of febrile Tanzanian children |
| title_fullStr | Detection of dicistroviruses RNA in blood of febrile Tanzanian children |
| title_full_unstemmed | Detection of dicistroviruses RNA in blood of febrile Tanzanian children |
| title_short | Detection of dicistroviruses RNA in blood of febrile Tanzanian children |
| title_sort | detection of dicistroviruses rna in blood of febrile tanzanian children |
| topic | Dicistrovirus de novo analysis sera viremia Tanzanian children |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1603791 |
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