HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency.
Lentiviruses such as HIV-1 traverse nuclear pore complexes (NPC) and infect terminally differentiated non-dividing cells, but how they do this is unclear. The cytoplasmic NPC protein Nup358/RanBP2 was identified as an HIV-1 co-factor in previous studies. Here we report that HIV-1 capsid (CA) binds d...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-12-01
|
| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002439&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849470723905552384 |
|---|---|
| author | Torsten Schaller Karen E Ocwieja Jane Rasaiyaah Amanda J Price Troy L Brady Shoshannah L Roth Stéphane Hué Adam J Fletcher KyeongEun Lee Vineet N KewalRamani Mahdad Noursadeghi Richard G Jenner Leo C James Frederic D Bushman Greg J Towers |
| author_facet | Torsten Schaller Karen E Ocwieja Jane Rasaiyaah Amanda J Price Troy L Brady Shoshannah L Roth Stéphane Hué Adam J Fletcher KyeongEun Lee Vineet N KewalRamani Mahdad Noursadeghi Richard G Jenner Leo C James Frederic D Bushman Greg J Towers |
| author_sort | Torsten Schaller |
| collection | DOAJ |
| description | Lentiviruses such as HIV-1 traverse nuclear pore complexes (NPC) and infect terminally differentiated non-dividing cells, but how they do this is unclear. The cytoplasmic NPC protein Nup358/RanBP2 was identified as an HIV-1 co-factor in previous studies. Here we report that HIV-1 capsid (CA) binds directly to the cyclophilin domain of Nup358/RanBP2. Fusion of the Nup358/RanBP2 cyclophilin (Cyp) domain to the tripartite motif of TRIM5 created a novel inhibitor of HIV-1 replication, consistent with an interaction in vivo. In contrast to CypA binding to HIV-1 CA, Nup358 binding is insensitive to inhibition with cyclosporine, allowing contributions from CypA and Nup358 to be distinguished. Inhibition of CypA reduced dependence on Nup358 and the nuclear basket protein Nup153, suggesting that CypA regulates the choice of the nuclear import machinery that is engaged by the virus. HIV-1 cyclophilin-binding mutants CA G89V and P90A favored integration in genomic regions with a higher density of transcription units and associated features than wild type virus. Integration preference of wild type virus in the presence of cyclosporine was similarly altered to regions of higher transcription density. In contrast, HIV-1 CA alterations in another patch on the capsid surface that render the virus less sensitive to Nup358 or TRN-SR2 depletion (CA N74D, N57A) resulted in integration in genomic regions sparse in transcription units. Both groups of CA mutants are impaired in replication in HeLa cells and human monocyte derived macrophages. Our findings link HIV-1 engagement of cyclophilins with both integration targeting and replication efficiency and provide insight into the conservation of viral cyclophilin recruitment. |
| format | Article |
| id | doaj-art-a5b5dc81b6be4ecc85627141155f796e |
| institution | Kabale University |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2011-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-a5b5dc81b6be4ecc85627141155f796e2025-08-20T03:25:04ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742011-12-01712e100243910.1371/journal.ppat.1002439HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency.Torsten SchallerKaren E OcwiejaJane RasaiyaahAmanda J PriceTroy L BradyShoshannah L RothStéphane HuéAdam J FletcherKyeongEun LeeVineet N KewalRamaniMahdad NoursadeghiRichard G JennerLeo C JamesFrederic D BushmanGreg J TowersLentiviruses such as HIV-1 traverse nuclear pore complexes (NPC) and infect terminally differentiated non-dividing cells, but how they do this is unclear. The cytoplasmic NPC protein Nup358/RanBP2 was identified as an HIV-1 co-factor in previous studies. Here we report that HIV-1 capsid (CA) binds directly to the cyclophilin domain of Nup358/RanBP2. Fusion of the Nup358/RanBP2 cyclophilin (Cyp) domain to the tripartite motif of TRIM5 created a novel inhibitor of HIV-1 replication, consistent with an interaction in vivo. In contrast to CypA binding to HIV-1 CA, Nup358 binding is insensitive to inhibition with cyclosporine, allowing contributions from CypA and Nup358 to be distinguished. Inhibition of CypA reduced dependence on Nup358 and the nuclear basket protein Nup153, suggesting that CypA regulates the choice of the nuclear import machinery that is engaged by the virus. HIV-1 cyclophilin-binding mutants CA G89V and P90A favored integration in genomic regions with a higher density of transcription units and associated features than wild type virus. Integration preference of wild type virus in the presence of cyclosporine was similarly altered to regions of higher transcription density. In contrast, HIV-1 CA alterations in another patch on the capsid surface that render the virus less sensitive to Nup358 or TRN-SR2 depletion (CA N74D, N57A) resulted in integration in genomic regions sparse in transcription units. Both groups of CA mutants are impaired in replication in HeLa cells and human monocyte derived macrophages. Our findings link HIV-1 engagement of cyclophilins with both integration targeting and replication efficiency and provide insight into the conservation of viral cyclophilin recruitment.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002439&type=printable |
| spellingShingle | Torsten Schaller Karen E Ocwieja Jane Rasaiyaah Amanda J Price Troy L Brady Shoshannah L Roth Stéphane Hué Adam J Fletcher KyeongEun Lee Vineet N KewalRamani Mahdad Noursadeghi Richard G Jenner Leo C James Frederic D Bushman Greg J Towers HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency. PLoS Pathogens |
| title | HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency. |
| title_full | HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency. |
| title_fullStr | HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency. |
| title_full_unstemmed | HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency. |
| title_short | HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency. |
| title_sort | hiv 1 capsid cyclophilin interactions determine nuclear import pathway integration targeting and replication efficiency |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002439&type=printable |
| work_keys_str_mv | AT torstenschaller hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT kareneocwieja hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT janerasaiyaah hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT amandajprice hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT troylbrady hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT shoshannahlroth hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT stephanehue hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT adamjfletcher hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT kyeongeunlee hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT vineetnkewalramani hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT mahdadnoursadeghi hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT richardgjenner hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT leocjames hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT fredericdbushman hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency AT gregjtowers hiv1capsidcyclophilininteractionsdeterminenuclearimportpathwayintegrationtargetingandreplicationefficiency |