Analysis of Halophilic Phenotypic Variation and Cytotoxicity of <i>Vibrio parahaemolyticus</i> from Different Sources

<i>Vibrio parahaemolyticus</i> is an aquatic animal pathogen. Recently, the detection rate of <i>V. parahaemolyticus</i> in freshwater products has exceeded that in seafood products, and the strains isolated from freshwater products exhibit better growth conditions in low-sal...

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Main Authors: Jingyue Gu, Xin Dong, Yunqian Zhou, Ying Zhao, Qiang Du, Jia Chen, Xujian Mao, Fengming Wang, Bowen Tu
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Pathogens
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Online Access:https://www.mdpi.com/2076-0817/14/2/182
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author Jingyue Gu
Xin Dong
Yunqian Zhou
Ying Zhao
Qiang Du
Jia Chen
Xujian Mao
Fengming Wang
Bowen Tu
author_facet Jingyue Gu
Xin Dong
Yunqian Zhou
Ying Zhao
Qiang Du
Jia Chen
Xujian Mao
Fengming Wang
Bowen Tu
author_sort Jingyue Gu
collection DOAJ
description <i>Vibrio parahaemolyticus</i> is an aquatic animal pathogen. Recently, the detection rate of <i>V. parahaemolyticus</i> in freshwater products has exceeded that in seafood products, and the strains isolated from freshwater products exhibit better growth conditions in low-salinity environments. This study is based on a food risk detection activity in Changzhou, Jiangsu Province, China, investigating the variation of halophilism and the virulence of two groups of strains under different salt concentrations. Under 0%, 0.5%, and 1% salt, the strains from the freshwater showed faster growth than those from the seawater. In comparison, the strains from the seawater group under 2% and 3% salt grew faster than the growing status under the foregoing low-salt concentration environment. The cytotoxicity produced by the two strains was approximately 1.4 times higher in the 0.5% and 1% salt concentration groups compared to the 3% corresponding experimental group. Under the 0%, 0.5%, and 1% salt, the cytotoxicity of strains in the freshwater group increased by nearly 20% compared to that in the seawater groups. The freshwater strains showed altered halophilism and adapted to the low-salt environment. This research will be helpful in establishing a local and global control strategy against the diseases resulting from <i>V. parahaemolyticus</i>.
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spelling doaj-art-a5aa754b418d4c4387e0f35c7a23f3d52025-08-20T02:04:02ZengMDPI AGPathogens2076-08172025-02-0114218210.3390/pathogens14020182Analysis of Halophilic Phenotypic Variation and Cytotoxicity of <i>Vibrio parahaemolyticus</i> from Different SourcesJingyue Gu0Xin Dong1Yunqian Zhou2Ying Zhao3Qiang Du4Jia Chen5Xujian Mao6Fengming Wang7Bowen Tu8School of Public Health, Xuzhou Medical University, Xuzhou 221004, ChinaChangzhou Centre for Disease Control and Prevention, Changzhou Medical Center, Nanjing Medical University, Changzhou 213000, ChinaSchool of Public Health, Nanjing Medical University, Nanjing 211166, ChinaChangzhou Centre for Disease Control and Prevention, Changzhou Medical Center, Nanjing Medical University, Changzhou 213000, ChinaChangzhou Centre for Disease Control and Prevention, Changzhou Medical Center, Nanjing Medical University, Changzhou 213000, ChinaChangzhou Centre for Disease Control and Prevention, Changzhou Medical Center, Nanjing Medical University, Changzhou 213000, ChinaChangzhou Centre for Disease Control and Prevention, Changzhou Medical Center, Nanjing Medical University, Changzhou 213000, ChinaChangzhou Centre for Disease Control and Prevention, Changzhou Medical Center, Nanjing Medical University, Changzhou 213000, ChinaSchool of Public Health, Xuzhou Medical University, Xuzhou 221004, China<i>Vibrio parahaemolyticus</i> is an aquatic animal pathogen. Recently, the detection rate of <i>V. parahaemolyticus</i> in freshwater products has exceeded that in seafood products, and the strains isolated from freshwater products exhibit better growth conditions in low-salinity environments. This study is based on a food risk detection activity in Changzhou, Jiangsu Province, China, investigating the variation of halophilism and the virulence of two groups of strains under different salt concentrations. Under 0%, 0.5%, and 1% salt, the strains from the freshwater showed faster growth than those from the seawater. In comparison, the strains from the seawater group under 2% and 3% salt grew faster than the growing status under the foregoing low-salt concentration environment. The cytotoxicity produced by the two strains was approximately 1.4 times higher in the 0.5% and 1% salt concentration groups compared to the 3% corresponding experimental group. Under the 0%, 0.5%, and 1% salt, the cytotoxicity of strains in the freshwater group increased by nearly 20% compared to that in the seawater groups. The freshwater strains showed altered halophilism and adapted to the low-salt environment. This research will be helpful in establishing a local and global control strategy against the diseases resulting from <i>V. parahaemolyticus</i>.https://www.mdpi.com/2076-0817/14/2/182foodborne diseasefreshwater productsaquatic animal pathogen evolutionhost-pathogen interactionvirulence alterationlow-saline water
spellingShingle Jingyue Gu
Xin Dong
Yunqian Zhou
Ying Zhao
Qiang Du
Jia Chen
Xujian Mao
Fengming Wang
Bowen Tu
Analysis of Halophilic Phenotypic Variation and Cytotoxicity of <i>Vibrio parahaemolyticus</i> from Different Sources
Pathogens
foodborne disease
freshwater products
aquatic animal pathogen evolution
host-pathogen interaction
virulence alteration
low-saline water
title Analysis of Halophilic Phenotypic Variation and Cytotoxicity of <i>Vibrio parahaemolyticus</i> from Different Sources
title_full Analysis of Halophilic Phenotypic Variation and Cytotoxicity of <i>Vibrio parahaemolyticus</i> from Different Sources
title_fullStr Analysis of Halophilic Phenotypic Variation and Cytotoxicity of <i>Vibrio parahaemolyticus</i> from Different Sources
title_full_unstemmed Analysis of Halophilic Phenotypic Variation and Cytotoxicity of <i>Vibrio parahaemolyticus</i> from Different Sources
title_short Analysis of Halophilic Phenotypic Variation and Cytotoxicity of <i>Vibrio parahaemolyticus</i> from Different Sources
title_sort analysis of halophilic phenotypic variation and cytotoxicity of i vibrio parahaemolyticus i from different sources
topic foodborne disease
freshwater products
aquatic animal pathogen evolution
host-pathogen interaction
virulence alteration
low-saline water
url https://www.mdpi.com/2076-0817/14/2/182
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