Isolation and Characterization of Two Coumarin Compounds from the Chloroform Fraction of <i>Scadoxus multiflorus</i> (Martyn) Raf. (Amaryllidaceae)

In this study, the aerial parts of <i>Scadoxus multiflorus</i> were extracted using methanol through a maceration process. The resulting methanol crude extract was subsequently partitioned with solvents including n-hexane, chloroform, ethyl acetate, and n-butanol. Extensive column chroma...

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Bibliographic Details
Main Authors: Olaiya Akeem Ayodele, Tijani Tawakaltu Omolara, Abdullahi Sakynah Musa, Sule Mohammed Ibrahim
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Chemistry Proceedings
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Online Access:https://www.mdpi.com/2673-4583/16/1/89
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Summary:In this study, the aerial parts of <i>Scadoxus multiflorus</i> were extracted using methanol through a maceration process. The resulting methanol crude extract was subsequently partitioned with solvents including n-hexane, chloroform, ethyl acetate, and n-butanol. Extensive column chromatography separation of the chloroform fraction, followed by isocratic elution of two pooled fractions, led to the isolation of two coumarin derivatives: 2-methyl-2H-chromen-7-ol and 7-methoxy-2H-chromen-2-one. These compounds underwent various physicochemical analyses, such as chemical tests, melting point determination, and solubility assessments. Structural elucidation of the isolated compounds was conducted using UV spectroscopy, FT-IR, and 1D/2D NMR techniques. The final molecular structures were confirmed and named using ChemDraw.
ISSN:2673-4583