Comparative Phytochemical Analysis and Antimicrobial Properties of Ethanol and Macerated Extracts from Aerial and Root Parts of <i>Achillea nobilis</i>

<i>Achillea nobilis</i> represents a species of considerable medicinal importance within the Asteraceae family, historically employed in Central Asia and various Eurasian territories for the management of inflammatory, microbial, and gastrointestinal ailments. Notwithstanding its extensi...

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Main Authors: Aiman Berdgaleeva, Zere Zhalimova, Akzharkyn Saginbazarova, Gulbanu Tulegenova, Dana Zharylkassynova, Aliya Bazargaliyeva, Zhaidargul Kuanbay, Svetlana Sakhanova, Akmaral Ramazanova, Akzhamal Bilkenova, Aigul Sartayeva
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/14/2957
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author Aiman Berdgaleeva
Zere Zhalimova
Akzharkyn Saginbazarova
Gulbanu Tulegenova
Dana Zharylkassynova
Aliya Bazargaliyeva
Zhaidargul Kuanbay
Svetlana Sakhanova
Akmaral Ramazanova
Akzhamal Bilkenova
Aigul Sartayeva
author_facet Aiman Berdgaleeva
Zere Zhalimova
Akzharkyn Saginbazarova
Gulbanu Tulegenova
Dana Zharylkassynova
Aliya Bazargaliyeva
Zhaidargul Kuanbay
Svetlana Sakhanova
Akmaral Ramazanova
Akzhamal Bilkenova
Aigul Sartayeva
author_sort Aiman Berdgaleeva
collection DOAJ
description <i>Achillea nobilis</i> represents a species of considerable medicinal importance within the Asteraceae family, historically employed in Central Asia and various Eurasian territories for the management of inflammatory, microbial, and gastrointestinal ailments. Notwithstanding its extensive ethnopharmacological significance, the phytochemical profile and pharmacological attributes of its various anatomical components have not been comprehensively investigated. This research endeavor sought to delineate the phytochemical constituents and evaluate the antimicrobial efficacy of ethanol extracts derived from both the aerial and root segments of <i>A. nobilis</i>. Qualitative phytochemical analysis and GC–MS characterization unveiled a diverse array of bioactive compounds, encompassing flavonoids, phenolic compounds, organic acids, lactones, alcohols, and heterocyclic derivatives. In particular, the aerial oil extract exhibited the presence of terpenoids, fatty acids and their esters, sterols, hydrocarbons, and minor organosilicon and cyclobutanone derivatives, with notable compounds such as linoleic acid (8.08%), 6-tetradecyne (14.99%), isopropyl linoleate (14.64%), and <i>E</i>,<i>Z</i>-1,3,12-nonadecatriene (22.25%). In vitro antimicrobial activity was assessed against eight clinically relevant microbial strains employing the broth microdilution technique. The aerial ethanol extract exhibited pronounced antimicrobial properties, particularly against MRSA and <i>C. albicans</i>, with MICs ranging from 0.5 to 2 mg/mL, whereas the root ethanol extract displayed MICs of 1 to 3 mg/mL. Additionally, the aerial oil extract showed moderate inhibitory activity, with MIC values ranging from 1.5 to 3 mg/mL, demonstrating effectiveness particularly against <i>C. albicans</i>, <i>C. neoformans</i>, and MRSA. These findings underscore the therapeutic potential of <i>A. nobilis</i>, particularly its aerial component, as a viable natural source of antimicrobial agents.
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spelling doaj-art-a5a208f0509d4fabb4c7977f1fba7ed32025-08-20T03:56:46ZengMDPI AGMolecules1420-30492025-07-013014295710.3390/molecules30142957Comparative Phytochemical Analysis and Antimicrobial Properties of Ethanol and Macerated Extracts from Aerial and Root Parts of <i>Achillea nobilis</i>Aiman Berdgaleeva0Zere Zhalimova1Akzharkyn Saginbazarova2Gulbanu Tulegenova3Dana Zharylkassynova4Aliya Bazargaliyeva5Zhaidargul Kuanbay6Svetlana Sakhanova7Akmaral Ramazanova8Akzhamal Bilkenova9Aigul Sartayeva10Department of Pharmaceutical Disciplines, Marat Ospanov West Kazakhstan Medical University, 66 Maresyev Street, Aktobe 030019, KazakhstanDepartment of Pharmaceutical Disciplines, Marat Ospanov West Kazakhstan Medical University, 66 Maresyev Street, Aktobe 030019, KazakhstanDepartment of Pharmaceutical Disciplines, Marat Ospanov West Kazakhstan Medical University, 66 Maresyev Street, Aktobe 030019, KazakhstanDepartment of Pharmaceutical Disciplines, Marat Ospanov West Kazakhstan Medical University, 66 Maresyev Street, Aktobe 030019, KazakhstanDepartment of Pharmaceutical Disciplines, Marat Ospanov West Kazakhstan Medical University, 66 Maresyev Street, Aktobe 030019, KazakhstanDepartment of Biology, Natural Sciences Faculty, K.Zhubanov Aktobe Regional University, 34 Moldagulova Avenue, Aktobe 030012, KazakhstanDepartment of Biology, Natural Sciences Faculty, K.Zhubanov Aktobe Regional University, 34 Moldagulova Avenue, Aktobe 030012, KazakhstanScientific and Practical Center, Marat Ospanov West Kazakhstan Medical University, 74 Maresyev Street, Aktobe 030019, KazakhstanDepartment of Neurology with the Course of Psychiatry and Narcology, Marat Ospanov West Kazakhstan Medical University, 66 Maresyev Street, Aktobe 030019, KazakhstanDepartment of Natural Sciences, Marat Ospanov West Kazakhstan Medical University, 66 Maresyev Street, Aktobe 030019, KazakhstanDepartment of General Medical Practice No. 2, Marat Ospanov West Kazakhstan Medical University, 66 Maresyev Street, Aktobe 030019, Kazakhstan<i>Achillea nobilis</i> represents a species of considerable medicinal importance within the Asteraceae family, historically employed in Central Asia and various Eurasian territories for the management of inflammatory, microbial, and gastrointestinal ailments. Notwithstanding its extensive ethnopharmacological significance, the phytochemical profile and pharmacological attributes of its various anatomical components have not been comprehensively investigated. This research endeavor sought to delineate the phytochemical constituents and evaluate the antimicrobial efficacy of ethanol extracts derived from both the aerial and root segments of <i>A. nobilis</i>. Qualitative phytochemical analysis and GC–MS characterization unveiled a diverse array of bioactive compounds, encompassing flavonoids, phenolic compounds, organic acids, lactones, alcohols, and heterocyclic derivatives. In particular, the aerial oil extract exhibited the presence of terpenoids, fatty acids and their esters, sterols, hydrocarbons, and minor organosilicon and cyclobutanone derivatives, with notable compounds such as linoleic acid (8.08%), 6-tetradecyne (14.99%), isopropyl linoleate (14.64%), and <i>E</i>,<i>Z</i>-1,3,12-nonadecatriene (22.25%). In vitro antimicrobial activity was assessed against eight clinically relevant microbial strains employing the broth microdilution technique. The aerial ethanol extract exhibited pronounced antimicrobial properties, particularly against MRSA and <i>C. albicans</i>, with MICs ranging from 0.5 to 2 mg/mL, whereas the root ethanol extract displayed MICs of 1 to 3 mg/mL. Additionally, the aerial oil extract showed moderate inhibitory activity, with MIC values ranging from 1.5 to 3 mg/mL, demonstrating effectiveness particularly against <i>C. albicans</i>, <i>C. neoformans</i>, and MRSA. These findings underscore the therapeutic potential of <i>A. nobilis</i>, particularly its aerial component, as a viable natural source of antimicrobial agents.https://www.mdpi.com/1420-3049/30/14/2957<i>Achillea nobilis</i>GC–MSvortex-assisted extractionethanol extractaerial and root partsphytochemical profiling
spellingShingle Aiman Berdgaleeva
Zere Zhalimova
Akzharkyn Saginbazarova
Gulbanu Tulegenova
Dana Zharylkassynova
Aliya Bazargaliyeva
Zhaidargul Kuanbay
Svetlana Sakhanova
Akmaral Ramazanova
Akzhamal Bilkenova
Aigul Sartayeva
Comparative Phytochemical Analysis and Antimicrobial Properties of Ethanol and Macerated Extracts from Aerial and Root Parts of <i>Achillea nobilis</i>
Molecules
<i>Achillea nobilis</i>
GC–MS
vortex-assisted extraction
ethanol extract
aerial and root parts
phytochemical profiling
title Comparative Phytochemical Analysis and Antimicrobial Properties of Ethanol and Macerated Extracts from Aerial and Root Parts of <i>Achillea nobilis</i>
title_full Comparative Phytochemical Analysis and Antimicrobial Properties of Ethanol and Macerated Extracts from Aerial and Root Parts of <i>Achillea nobilis</i>
title_fullStr Comparative Phytochemical Analysis and Antimicrobial Properties of Ethanol and Macerated Extracts from Aerial and Root Parts of <i>Achillea nobilis</i>
title_full_unstemmed Comparative Phytochemical Analysis and Antimicrobial Properties of Ethanol and Macerated Extracts from Aerial and Root Parts of <i>Achillea nobilis</i>
title_short Comparative Phytochemical Analysis and Antimicrobial Properties of Ethanol and Macerated Extracts from Aerial and Root Parts of <i>Achillea nobilis</i>
title_sort comparative phytochemical analysis and antimicrobial properties of ethanol and macerated extracts from aerial and root parts of i achillea nobilis i
topic <i>Achillea nobilis</i>
GC–MS
vortex-assisted extraction
ethanol extract
aerial and root parts
phytochemical profiling
url https://www.mdpi.com/1420-3049/30/14/2957
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