Programmable Macrophage Vesicle Based Bionic Self‐Adjuvanting Vaccine for Immunization against Monkeypox Virus
Abstract The emergence of monkeypox has become a global health threat after the COVID‐19 pandemic. Due to the lack of available specifically treatment against MPV, developing an available vaccine is thus the most prospective and urgent strategy. Herein, a programmable macrophage vesicle based bionic...
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202408608 |
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| author | Weiqiang Lin Chenguang Shen Mengjun Li Shengchao Ma Chenxin Liu Jialin Huang Zuning Ren Yuechao Yang Minghai Zhao Qiulin Xie Shuang Guo Wei Wang Kaiyuan Wang Qiang Ma Yideng Jiang Judun Zheng Yuhui Liao |
| author_facet | Weiqiang Lin Chenguang Shen Mengjun Li Shengchao Ma Chenxin Liu Jialin Huang Zuning Ren Yuechao Yang Minghai Zhao Qiulin Xie Shuang Guo Wei Wang Kaiyuan Wang Qiang Ma Yideng Jiang Judun Zheng Yuhui Liao |
| author_sort | Weiqiang Lin |
| collection | DOAJ |
| description | Abstract The emergence of monkeypox has become a global health threat after the COVID‐19 pandemic. Due to the lack of available specifically treatment against MPV, developing an available vaccine is thus the most prospective and urgent strategy. Herein, a programmable macrophage vesicle based bionic self‐adjuvanting vaccine (AM@AEvs‐PB) is first developed for defending against monkeypox virus (MPV). Based on MPV‐related antigen‐stimulated macrophage‐derived vesicles, the nanovaccine is constructed by loading the mature virion (MV)‐related intracellular protein (A29L/M1R) and simultaneously modifying with the enveloped virion (EV) antigen (B6R), enabling them to effectively promote antigen presentation and enhance adaptive immune through self‐adjuvant strategy. Owing to the synergistic properties of bionic vaccine coloaded MV and EV protein in defensing MPV, the activation ratio of antigen‐presenting cells is nearly four times than that of single antigen in the same dose, resulting in stronger immunity in host. Notably, intramuscular injection uptake of AM@AEvs‐PB demonstrated vigorous immune‐protective effects in the mouse challenge attempt, offering a promising strategy for pre‐clinical monkeypox vaccine development. |
| format | Article |
| id | doaj-art-a59738d977734b20bf3d949ab6636e90 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-a59738d977734b20bf3d949ab6636e902025-01-09T11:44:45ZengWileyAdvanced Science2198-38442025-01-01121n/an/a10.1002/advs.202408608Programmable Macrophage Vesicle Based Bionic Self‐Adjuvanting Vaccine for Immunization against Monkeypox VirusWeiqiang Lin0Chenguang Shen1Mengjun Li2Shengchao Ma3Chenxin Liu4Jialin Huang5Zuning Ren6Yuechao Yang7Minghai Zhao8Qiulin Xie9Shuang Guo10Wei Wang11Kaiyuan Wang12Qiang Ma13Yideng Jiang14Judun Zheng15Yuhui Liao16NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Key Laboratory of Vascular Injury and Repair Research Ningxia Medical University Yinchuan 750004 P. R. ChinaBSL‐3 Laboratory (Guangdong), Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University Guangzhou 510515 P. R. ChinaBSL‐3 Laboratory (Guangdong), Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University Guangzhou 510515 P. R. ChinaNHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Key Laboratory of Vascular Injury and Repair Research Ningxia Medical University Yinchuan 750004 P. R. ChinaSchool of Laboratory Medicine and Biotechnology Southern Medical University Guangzhou 510515 P. R. ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital, Southern Medical University Guangzhou 510091 P. R. ChinaBSL‐3 Laboratory (Guangdong), Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University Guangzhou 510515 P. R. ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital, Southern Medical University Guangzhou 510091 P. R. ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital, Southern Medical University Guangzhou 510091 P. R. ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital, Southern Medical University Guangzhou 510091 P. R. ChinaNHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Key Laboratory of Vascular Injury and Repair Research Ningxia Medical University Yinchuan 750004 P. R. ChinaBSL‐3 Laboratory (Guangdong), Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University Guangzhou 510515 P. R. ChinaDepartments of Diagnostic Radiology, Surgery, Chemical and Biomolecular Engineering, and Biomedical Engineering, Yong Loo Lin School of Medicine and College of Design and Engineering National University of Singapore Singapore 119074 SingaporeSchool of Laboratory Medicine and Biotechnology Southern Medical University Guangzhou 510515 P. R. ChinaNHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Key Laboratory of Vascular Injury and Repair Research Ningxia Medical University Yinchuan 750004 P. R. ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital, Southern Medical University Guangzhou 510091 P. R. ChinaNHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Key Laboratory of Vascular Injury and Repair Research Ningxia Medical University Yinchuan 750004 P. R. ChinaAbstract The emergence of monkeypox has become a global health threat after the COVID‐19 pandemic. Due to the lack of available specifically treatment against MPV, developing an available vaccine is thus the most prospective and urgent strategy. Herein, a programmable macrophage vesicle based bionic self‐adjuvanting vaccine (AM@AEvs‐PB) is first developed for defending against monkeypox virus (MPV). Based on MPV‐related antigen‐stimulated macrophage‐derived vesicles, the nanovaccine is constructed by loading the mature virion (MV)‐related intracellular protein (A29L/M1R) and simultaneously modifying with the enveloped virion (EV) antigen (B6R), enabling them to effectively promote antigen presentation and enhance adaptive immune through self‐adjuvant strategy. Owing to the synergistic properties of bionic vaccine coloaded MV and EV protein in defensing MPV, the activation ratio of antigen‐presenting cells is nearly four times than that of single antigen in the same dose, resulting in stronger immunity in host. Notably, intramuscular injection uptake of AM@AEvs‐PB demonstrated vigorous immune‐protective effects in the mouse challenge attempt, offering a promising strategy for pre‐clinical monkeypox vaccine development.https://doi.org/10.1002/advs.202408608bionic vaccineextracellular enveloped virionintracellular mature virionmonkeypox virusmacrophage vesicles |
| spellingShingle | Weiqiang Lin Chenguang Shen Mengjun Li Shengchao Ma Chenxin Liu Jialin Huang Zuning Ren Yuechao Yang Minghai Zhao Qiulin Xie Shuang Guo Wei Wang Kaiyuan Wang Qiang Ma Yideng Jiang Judun Zheng Yuhui Liao Programmable Macrophage Vesicle Based Bionic Self‐Adjuvanting Vaccine for Immunization against Monkeypox Virus Advanced Science bionic vaccine extracellular enveloped virion intracellular mature virion monkeypox virus macrophage vesicles |
| title | Programmable Macrophage Vesicle Based Bionic Self‐Adjuvanting Vaccine for Immunization against Monkeypox Virus |
| title_full | Programmable Macrophage Vesicle Based Bionic Self‐Adjuvanting Vaccine for Immunization against Monkeypox Virus |
| title_fullStr | Programmable Macrophage Vesicle Based Bionic Self‐Adjuvanting Vaccine for Immunization against Monkeypox Virus |
| title_full_unstemmed | Programmable Macrophage Vesicle Based Bionic Self‐Adjuvanting Vaccine for Immunization against Monkeypox Virus |
| title_short | Programmable Macrophage Vesicle Based Bionic Self‐Adjuvanting Vaccine for Immunization against Monkeypox Virus |
| title_sort | programmable macrophage vesicle based bionic self adjuvanting vaccine for immunization against monkeypox virus |
| topic | bionic vaccine extracellular enveloped virion intracellular mature virion monkeypox virus macrophage vesicles |
| url | https://doi.org/10.1002/advs.202408608 |
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