Protocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays

Protocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays

Summary: Voltage-dependent anion channel 1 (VDAC1) is a key protein in cellular metabolism and apoptosis. Here, we present a protocol to express and purify milligram amounts of recombinant VDAC1 in Escherichia coli. We detail steps for a fluorescence polarization-based high-throughput screening assa...

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Bibliographic Details
Main Authors: Stefano Conti Nibali, Andrea Magrì, Angela Messina, Armin Wagner, Ramona Duman, Vito De Pinto, Cristian Turato, Cristina Arrigoni, Marco Lolicato
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Biophysics
Protein Biochemistry
Protein expression and purification
X-ray Crystallography
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007226
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