CGRP Inhibits Apoptosis and Protects against Extraocular Muscle Atrophy in Patients with Concomitant Exotropia through the AKT/CREB Pathway
Concomitant exotropia is a common chronic eye disease characterized by abnormal movement of the extraocular muscles caused by a functional imbalance of the nerves related to the movement of the extraocular muscles. This study revealed that the expression levels of S-100β and the muscle area decrease...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2025-01-01
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| Series: | Journal of Bio-X Research |
| Online Access: | https://spj.science.org/doi/10.34133/jbioxresearch.0055 |
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| author | Xiaoping Hong Fadian Ding Xingting Chen Feng Gao Qicai Liu |
| author_facet | Xiaoping Hong Fadian Ding Xingting Chen Feng Gao Qicai Liu |
| author_sort | Xiaoping Hong |
| collection | DOAJ |
| description | Concomitant exotropia is a common chronic eye disease characterized by abnormal movement of the extraocular muscles caused by a functional imbalance of the nerves related to the movement of the extraocular muscles. This study revealed that the expression levels of S-100β and the muscle area decreased in the extraocular muscles of patients with concomitant exotropia, whereas the expression of calcitonin gene-related peptide (CGRP) increased. In CGRP-KO rats, low expression of CGRP was positively correlated with muscle atrophy. Moreover, the expression level of muscle cells, which are fast myosin heavy chain positive and slow myosin positive in CGRP-KO rats, was lower than that in the control group. Drug intervention experiments further confirmed the inhibitory effect of CGRP on muscle atrophy. The level of apoptosis in the extraocular muscles of CGRP-KO rats was significantly greater than that in the control group (P < 0.05), and the phosphorylation level of AKT/CREB in the extraocular muscles of patients with concomitant exotropia was greater than that in the control group. The increased expression of CGRP in the extraocular muscles of patients with concomitant exotropia may inhibit the apoptosis of extraocular muscle cells through the AKT/CREB signaling pathway and participate in the protective effect of the extraocular muscles in concomitant exotropia. |
| format | Article |
| id | doaj-art-a59082a9031f46c8a8144299a802da66 |
| institution | Kabale University |
| issn | 2577-3585 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Journal of Bio-X Research |
| spelling | doaj-art-a59082a9031f46c8a8144299a802da662025-08-20T04:00:41ZengAmerican Association for the Advancement of Science (AAAS)Journal of Bio-X Research2577-35852025-01-01810.34133/jbioxresearch.0055CGRP Inhibits Apoptosis and Protects against Extraocular Muscle Atrophy in Patients with Concomitant Exotropia through the AKT/CREB PathwayXiaoping Hong0Fadian Ding1Xingting Chen2Feng Gao3Qicai Liu4Department of Ophthalmology, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, P. R. China.Hepatopancreatobiliary Surgery Department, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, P. R. China.Fujian Provincial Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Medical University, Fuzhou, P. R. China.Department of Pathology, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, P. R. China.Fujian Provincial Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Medical University, Fuzhou, P. R. China.Concomitant exotropia is a common chronic eye disease characterized by abnormal movement of the extraocular muscles caused by a functional imbalance of the nerves related to the movement of the extraocular muscles. This study revealed that the expression levels of S-100β and the muscle area decreased in the extraocular muscles of patients with concomitant exotropia, whereas the expression of calcitonin gene-related peptide (CGRP) increased. In CGRP-KO rats, low expression of CGRP was positively correlated with muscle atrophy. Moreover, the expression level of muscle cells, which are fast myosin heavy chain positive and slow myosin positive in CGRP-KO rats, was lower than that in the control group. Drug intervention experiments further confirmed the inhibitory effect of CGRP on muscle atrophy. The level of apoptosis in the extraocular muscles of CGRP-KO rats was significantly greater than that in the control group (P < 0.05), and the phosphorylation level of AKT/CREB in the extraocular muscles of patients with concomitant exotropia was greater than that in the control group. The increased expression of CGRP in the extraocular muscles of patients with concomitant exotropia may inhibit the apoptosis of extraocular muscle cells through the AKT/CREB signaling pathway and participate in the protective effect of the extraocular muscles in concomitant exotropia.https://spj.science.org/doi/10.34133/jbioxresearch.0055 |
| spellingShingle | Xiaoping Hong Fadian Ding Xingting Chen Feng Gao Qicai Liu CGRP Inhibits Apoptosis and Protects against Extraocular Muscle Atrophy in Patients with Concomitant Exotropia through the AKT/CREB Pathway Journal of Bio-X Research |
| title | CGRP Inhibits Apoptosis and Protects against Extraocular Muscle Atrophy in Patients with Concomitant Exotropia through the AKT/CREB Pathway |
| title_full | CGRP Inhibits Apoptosis and Protects against Extraocular Muscle Atrophy in Patients with Concomitant Exotropia through the AKT/CREB Pathway |
| title_fullStr | CGRP Inhibits Apoptosis and Protects against Extraocular Muscle Atrophy in Patients with Concomitant Exotropia through the AKT/CREB Pathway |
| title_full_unstemmed | CGRP Inhibits Apoptosis and Protects against Extraocular Muscle Atrophy in Patients with Concomitant Exotropia through the AKT/CREB Pathway |
| title_short | CGRP Inhibits Apoptosis and Protects against Extraocular Muscle Atrophy in Patients with Concomitant Exotropia through the AKT/CREB Pathway |
| title_sort | cgrp inhibits apoptosis and protects against extraocular muscle atrophy in patients with concomitant exotropia through the akt creb pathway |
| url | https://spj.science.org/doi/10.34133/jbioxresearch.0055 |
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