Chrysanthemum indicum Flavonoid Extract on the Oxidative Stress Resistance of Caenorhabditis elegans
To investigate the in vivo antioxidant activity of flavonoid extract from Chrysanthemum indicum and its effects on oxidative stress resistance, this study evaluated the effects of Chrysanthemum indicum flavonoid extract on the antioxidant enzyme activities and the mean lifespan of Caenorhabditis ele...
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The editorial department of Science and Technology of Food Industry
2025-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070078 |
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| author | Hailin CUI Hailun TANG Lian LIU Zuohao LI Jingjing XIANG Rong LI |
| author_facet | Hailin CUI Hailun TANG Lian LIU Zuohao LI Jingjing XIANG Rong LI |
| author_sort | Hailin CUI |
| collection | DOAJ |
| description | To investigate the in vivo antioxidant activity of flavonoid extract from Chrysanthemum indicum and its effects on oxidative stress resistance, this study evaluated the effects of Chrysanthemum indicum flavonoid extract on the antioxidant enzyme activities and the mean lifespan of Caenorhabditis elegans (C. elegans) under oxidative stress. Moreover, the mechanisms of enhancing oxidative stress resistance in nematodes were explored by utilizing a variety of transgenic strains. The results showed that pretreatment with Chrysanthemum indicum flavonoid extract significantly prolonged the mean lifespan of worms under oxidative stress by 14.00%, 27.15%, and 18.65% (P<0.01) at concentrations of 50, 200, and 300 μg/mL, respectively. In addition, Chrysanthemum indicum flavonoid extract significantly increased the SOD activity and GSH level of C. elegans (P<0.01). Furthermore, the nuclear proportion of SKN-1 was increased from 17.16% to 51.46% in LD1 strains when treated with 200 μg/mL Chrysanthemum indicum flavonoid extract. Additionally, the results of lifespan assay of EU1 strains indicated that the lifespan extension effect of Chrysanthemum indicum flavonoid extract under oxidative stress was relied on skn-1. The mechanism of enhancing oxidative stress resistance of Chrysanthemum indicum flavonoid extract might occur by activating the transcription factor SKN-1, which in turn increased the expression of antioxidant-related proteins SOD-3, GCS-1 and GST-4. This study provides a scientific basis for the application of Chrysanthemum indicum flavonoids in alleviating oxidative stress damage. |
| format | Article |
| id | doaj-art-a58ea7146895490ba66cddcd68d8977c |
| institution | OA Journals |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-a58ea7146895490ba66cddcd68d8977c2025-08-20T01:55:34ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-06-01461133834510.13386/j.issn1002-0306.20240700782024070078-11Chrysanthemum indicum Flavonoid Extract on the Oxidative Stress Resistance of Caenorhabditis elegansHailin CUI0Hailun TANG1Lian LIU2Zuohao LI3Jingjing XIANG4Rong LI5Characteristic Food Function Mining and Comprehensive Utilization Research Center, Jingchu University of Technology, Jingmen 448000, ChinaCharacteristic Food Function Mining and Comprehensive Utilization Research Center, Jingchu University of Technology, Jingmen 448000, ChinaCharacteristic Food Function Mining and Comprehensive Utilization Research Center, Jingchu University of Technology, Jingmen 448000, ChinaCharacteristic Food Function Mining and Comprehensive Utilization Research Center, Jingchu University of Technology, Jingmen 448000, ChinaCharacteristic Food Function Mining and Comprehensive Utilization Research Center, Jingchu University of Technology, Jingmen 448000, ChinaCharacteristic Food Function Mining and Comprehensive Utilization Research Center, Jingchu University of Technology, Jingmen 448000, ChinaTo investigate the in vivo antioxidant activity of flavonoid extract from Chrysanthemum indicum and its effects on oxidative stress resistance, this study evaluated the effects of Chrysanthemum indicum flavonoid extract on the antioxidant enzyme activities and the mean lifespan of Caenorhabditis elegans (C. elegans) under oxidative stress. Moreover, the mechanisms of enhancing oxidative stress resistance in nematodes were explored by utilizing a variety of transgenic strains. The results showed that pretreatment with Chrysanthemum indicum flavonoid extract significantly prolonged the mean lifespan of worms under oxidative stress by 14.00%, 27.15%, and 18.65% (P<0.01) at concentrations of 50, 200, and 300 μg/mL, respectively. In addition, Chrysanthemum indicum flavonoid extract significantly increased the SOD activity and GSH level of C. elegans (P<0.01). Furthermore, the nuclear proportion of SKN-1 was increased from 17.16% to 51.46% in LD1 strains when treated with 200 μg/mL Chrysanthemum indicum flavonoid extract. Additionally, the results of lifespan assay of EU1 strains indicated that the lifespan extension effect of Chrysanthemum indicum flavonoid extract under oxidative stress was relied on skn-1. The mechanism of enhancing oxidative stress resistance of Chrysanthemum indicum flavonoid extract might occur by activating the transcription factor SKN-1, which in turn increased the expression of antioxidant-related proteins SOD-3, GCS-1 and GST-4. This study provides a scientific basis for the application of Chrysanthemum indicum flavonoids in alleviating oxidative stress damage.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070078chrysanthemum indicum flavonoid extractcaenorhabditis elegansantioxidantoxidative stress resistance |
| spellingShingle | Hailin CUI Hailun TANG Lian LIU Zuohao LI Jingjing XIANG Rong LI Chrysanthemum indicum Flavonoid Extract on the Oxidative Stress Resistance of Caenorhabditis elegans Shipin gongye ke-ji chrysanthemum indicum flavonoid extract caenorhabditis elegans antioxidant oxidative stress resistance |
| title | Chrysanthemum indicum Flavonoid Extract on the Oxidative Stress Resistance of Caenorhabditis elegans |
| title_full | Chrysanthemum indicum Flavonoid Extract on the Oxidative Stress Resistance of Caenorhabditis elegans |
| title_fullStr | Chrysanthemum indicum Flavonoid Extract on the Oxidative Stress Resistance of Caenorhabditis elegans |
| title_full_unstemmed | Chrysanthemum indicum Flavonoid Extract on the Oxidative Stress Resistance of Caenorhabditis elegans |
| title_short | Chrysanthemum indicum Flavonoid Extract on the Oxidative Stress Resistance of Caenorhabditis elegans |
| title_sort | chrysanthemum indicum flavonoid extract on the oxidative stress resistance of caenorhabditis elegans |
| topic | chrysanthemum indicum flavonoid extract caenorhabditis elegans antioxidant oxidative stress resistance |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070078 |
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