Ultrafast and Ultrasensitive Simultaneous Molecular Recognition and Quantification of CA12-5, CA72-4, HER1, and AFP in Biological Samples
Simultaneous molecular recognition and quantification of at least four biomarkers in biological samples may contribute to early and fast diagnosis of illnesses such as cancer. The electrodes able to reliably perform on-site these tests are the stochastic sensors. Therefore, three novel 3D stochastic...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/13/6/210 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849432737780334592 |
|---|---|
| author | Ruxandra-Maria Ilie-Mihai Raluca-Ioana Stefan-van Staden Bianca-Maria Tuchiu-Stanca |
| author_facet | Ruxandra-Maria Ilie-Mihai Raluca-Ioana Stefan-van Staden Bianca-Maria Tuchiu-Stanca |
| author_sort | Ruxandra-Maria Ilie-Mihai |
| collection | DOAJ |
| description | Simultaneous molecular recognition and quantification of at least four biomarkers in biological samples may contribute to early and fast diagnosis of illnesses such as cancer. The electrodes able to reliably perform on-site these tests are the stochastic sensors. Therefore, three novel 3D stochastic sensors employing carbon-based powders (graphite, graphene, nanographene) treated with N-(2-mercapto-1H-benzo[d]imidazole-5-yl) oleamide solution were used for screening tests of whole blood, gastric tumoral tissue, urine, and saliva for molecular recognition and quantification of CA12-5, CA72-4, HER1, and AFP. The best performance was achieved for the sensor based on graphene, when the highest sensitivities were recorded, on wide working concentration ranges of: 8.37 × 10<sup>−14</sup>–8.37 U mL<sup>−1</sup> for CA12-5, 4.00 × 10<sup>−11</sup>–4.00 × 10<sup>−3</sup> U mL<sup>−1</sup> for CA72-4, 3.90 × 10<sup>−16</sup>–3.90 × 10<sup>−6</sup> g mL<sup>−1</sup> for HER1, and 3.00 × 10<sup>−20</sup>–3.00 × 10<sup>−6</sup> g mL<sup>−1</sup> for AFP. The wide linear concentration ranges cover levels of biomarkers found in gastric cancer patients from early to late stages. The recovery values were higher than 98.00 with %, RSD lower than 1.00%. |
| format | Article |
| id | doaj-art-a5894544edec4dfdb232cb0ec53d1672 |
| institution | Kabale University |
| issn | 2227-9040 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj-art-a5894544edec4dfdb232cb0ec53d16722025-08-20T03:27:17ZengMDPI AGChemosensors2227-90402025-06-0113621010.3390/chemosensors13060210Ultrafast and Ultrasensitive Simultaneous Molecular Recognition and Quantification of CA12-5, CA72-4, HER1, and AFP in Biological SamplesRuxandra-Maria Ilie-Mihai0Raluca-Ioana Stefan-van Staden1Bianca-Maria Tuchiu-Stanca2Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021 Bucharest, RomaniaLaboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021 Bucharest, RomaniaLaboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021 Bucharest, RomaniaSimultaneous molecular recognition and quantification of at least four biomarkers in biological samples may contribute to early and fast diagnosis of illnesses such as cancer. The electrodes able to reliably perform on-site these tests are the stochastic sensors. Therefore, three novel 3D stochastic sensors employing carbon-based powders (graphite, graphene, nanographene) treated with N-(2-mercapto-1H-benzo[d]imidazole-5-yl) oleamide solution were used for screening tests of whole blood, gastric tumoral tissue, urine, and saliva for molecular recognition and quantification of CA12-5, CA72-4, HER1, and AFP. The best performance was achieved for the sensor based on graphene, when the highest sensitivities were recorded, on wide working concentration ranges of: 8.37 × 10<sup>−14</sup>–8.37 U mL<sup>−1</sup> for CA12-5, 4.00 × 10<sup>−11</sup>–4.00 × 10<sup>−3</sup> U mL<sup>−1</sup> for CA72-4, 3.90 × 10<sup>−16</sup>–3.90 × 10<sup>−6</sup> g mL<sup>−1</sup> for HER1, and 3.00 × 10<sup>−20</sup>–3.00 × 10<sup>−6</sup> g mL<sup>−1</sup> for AFP. The wide linear concentration ranges cover levels of biomarkers found in gastric cancer patients from early to late stages. The recovery values were higher than 98.00 with %, RSD lower than 1.00%.https://www.mdpi.com/2227-9040/13/6/210CA12-5CA72-4HER-1AFPstochastic sensor |
| spellingShingle | Ruxandra-Maria Ilie-Mihai Raluca-Ioana Stefan-van Staden Bianca-Maria Tuchiu-Stanca Ultrafast and Ultrasensitive Simultaneous Molecular Recognition and Quantification of CA12-5, CA72-4, HER1, and AFP in Biological Samples Chemosensors CA12-5 CA72-4 HER-1 AFP stochastic sensor |
| title | Ultrafast and Ultrasensitive Simultaneous Molecular Recognition and Quantification of CA12-5, CA72-4, HER1, and AFP in Biological Samples |
| title_full | Ultrafast and Ultrasensitive Simultaneous Molecular Recognition and Quantification of CA12-5, CA72-4, HER1, and AFP in Biological Samples |
| title_fullStr | Ultrafast and Ultrasensitive Simultaneous Molecular Recognition and Quantification of CA12-5, CA72-4, HER1, and AFP in Biological Samples |
| title_full_unstemmed | Ultrafast and Ultrasensitive Simultaneous Molecular Recognition and Quantification of CA12-5, CA72-4, HER1, and AFP in Biological Samples |
| title_short | Ultrafast and Ultrasensitive Simultaneous Molecular Recognition and Quantification of CA12-5, CA72-4, HER1, and AFP in Biological Samples |
| title_sort | ultrafast and ultrasensitive simultaneous molecular recognition and quantification of ca12 5 ca72 4 her1 and afp in biological samples |
| topic | CA12-5 CA72-4 HER-1 AFP stochastic sensor |
| url | https://www.mdpi.com/2227-9040/13/6/210 |
| work_keys_str_mv | AT ruxandramariailiemihai ultrafastandultrasensitivesimultaneousmolecularrecognitionandquantificationofca125ca724her1andafpinbiologicalsamples AT ralucaioanastefanvanstaden ultrafastandultrasensitivesimultaneousmolecularrecognitionandquantificationofca125ca724her1andafpinbiologicalsamples AT biancamariatuchiustanca ultrafastandultrasensitivesimultaneousmolecularrecognitionandquantificationofca125ca724her1andafpinbiologicalsamples |