THE EFFECTIVENESS OF COMPOSTING USING STABILIZING URBAN SEWAGE SLUDGE COMPOUNDS

The purpose of this investigation was to verify the effectiveness of composting in the sanitization of sludge from urban sewage (SS). The treatments (T) used consisted of SS mixed with sugarcane bagasse (SB), tree pruning residues (TP), poultry litter (PL) or grass clipping (GC) at a carbon/nitrogen...

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Main Authors: Liza OGAWA, Leopoldo Sussumu MATSUMOTO, Roberta DOS SANTOS TOLEDO, Jonatas CAMPOS DE ALMEIDA, Victor Bittencourt Dutra TABACOW, Fernanda Maria DE OLIVEIRA DIAS, Diego Resende RODRIGUES, Thaís Monica CABRAL, Roberta Lemos FREIRE, Italmar Teodorico NAVARRO
Format: Article
Language:English
Published: "Ion Ionescu de la Brad" Iasi University of Life Sciences 2023-07-01
Series:Journal of Applied Life Sciences and Environment
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Online Access:https://jurnalalse.com/wp-content/uploads/ALSE2-2023-01.pdf
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author Liza OGAWA
Leopoldo Sussumu MATSUMOTO
Roberta DOS SANTOS TOLEDO
Jonatas CAMPOS DE ALMEIDA
Victor Bittencourt Dutra TABACOW
Fernanda Maria DE OLIVEIRA DIAS
Diego Resende RODRIGUES
Thaís Monica CABRAL
Roberta Lemos FREIRE
Italmar Teodorico NAVARRO
author_facet Liza OGAWA
Leopoldo Sussumu MATSUMOTO
Roberta DOS SANTOS TOLEDO
Jonatas CAMPOS DE ALMEIDA
Victor Bittencourt Dutra TABACOW
Fernanda Maria DE OLIVEIRA DIAS
Diego Resende RODRIGUES
Thaís Monica CABRAL
Roberta Lemos FREIRE
Italmar Teodorico NAVARRO
author_sort Liza OGAWA
collection DOAJ
description The purpose of this investigation was to verify the effectiveness of composting in the sanitization of sludge from urban sewage (SS). The treatments (T) used consisted of SS mixed with sugarcane bagasse (SB), tree pruning residues (TP), poultry litter (PL) or grass clipping (GC) at a carbon/nitrogen ratio of approximately 30/1 and ratios of T1 (SS+SB), T2 (SS+SB+TP), T3 (SS+SB+GC), T4 (SS+SB+PL) or T5 (SS+SB+TP+GC). Temperature was measured daily for over 280 days of composting. Every two weeks, the compounds were analyzed for thermotolerant coliforms and viable helminth eggs using the multiple-tube and Yanko techniques; and once each month to identify Cryptosporidium spp. and Giardia spp. (oo)cysts using the sucrose and zinc sulfate centrifugal flotation technique and nested PCR followed by sequencing. The maximum temperatures measured in the thermophilic phase of the compounds ranged from 55°C to 64.8°C, and at day 280, weight reductions of 27% (T1), 48% (T2), 63% (T3), 66% (T4) and 64% (T5) were observed. The absence of fecal coliforms was observed from day 98 (T4), day 126 (T1), day 196 (T3 and T5) and day 210 (T2). All treatments resulted in helminth egg inviability and the absence of protozoan (oo)cysts. Only T4 and T5 were positive for G. duodenalis according to the sequencing analysis. In conclusion, high temperatures during the composting thermophilic phase made the use of pathogens required by legislation unfeasible; therefore, it is important to investigate the viability of protozoa in compounds to ensure a safe final product for human and animal health.
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spelling doaj-art-a575eb5ad3bb4b19a1793f76bbb573842025-08-20T03:38:30Zeng"Ion Ionescu de la Brad" Iasi University of Life SciencesJournal of Applied Life Sciences and Environment2784-03792784-03602023-07-01562(194)139151https://doi.org/10.46909/alse-562091THE EFFECTIVENESS OF COMPOSTING USING STABILIZING URBAN SEWAGE SLUDGE COMPOUNDSLiza OGAWA0Leopoldo Sussumu MATSUMOTO1Roberta DOS SANTOS TOLEDO2Jonatas CAMPOS DE ALMEIDA3Victor Bittencourt Dutra TABACOW4Fernanda Maria DE OLIVEIRA DIAS5Diego Resende RODRIGUES6Thaís Monica CABRAL7Roberta Lemos FREIRE8Italmar Teodorico NAVARRO9Universidade Estadual do Norte do Paraná, Campus Luiz Meneghel. UENP/CLM. Caixa Postal 261, CEP 86360-000, Bandeirantes, Paraná, BrasilUniversidade Estadual do Norte do Paraná, Campus Luiz Meneghel. UENP/CLM. Caixa Postal 261, CEP 86360-000, Bandeirantes, Paraná, BrasilUniversidade Estadual de Londrina, Departamento de Medicina Veterinária Preventiva, Londrina, Paraná, BrasilUniversidade Estadual de Londrina, Departamento de Medicina Veterinária Preventiva, Londrina, Paraná, BrasilUniversidade Estadual de Londrina, Departamento de Medicina Veterinária Preventiva, Londrina, Paraná, Brasil Universidade Estadual do Norte do Paraná, Campus Luiz Meneghel. UENP/CLM. Caixa Postal 261, CEP 86360-000, Bandeirantes, Paraná, BrasilUniversidade Estadual do Norte do Paraná, Campus Luiz Meneghel. UENP/CLM. Caixa Postal 261, CEP 86360-000, Bandeirantes, Paraná, BrasilUniversidade Estadual de Londrina, Departamento de Medicina Veterinária Preventiva, Londrina, Paraná, BrasilUniversidade Estadual de Londrina, Departamento de Medicina Veterinária Preventiva, Londrina, Paraná, BrasilUniversidade Estadual de Londrina, Departamento de Medicina Veterinária Preventiva, Londrina, Paraná, BrasilThe purpose of this investigation was to verify the effectiveness of composting in the sanitization of sludge from urban sewage (SS). The treatments (T) used consisted of SS mixed with sugarcane bagasse (SB), tree pruning residues (TP), poultry litter (PL) or grass clipping (GC) at a carbon/nitrogen ratio of approximately 30/1 and ratios of T1 (SS+SB), T2 (SS+SB+TP), T3 (SS+SB+GC), T4 (SS+SB+PL) or T5 (SS+SB+TP+GC). Temperature was measured daily for over 280 days of composting. Every two weeks, the compounds were analyzed for thermotolerant coliforms and viable helminth eggs using the multiple-tube and Yanko techniques; and once each month to identify Cryptosporidium spp. and Giardia spp. (oo)cysts using the sucrose and zinc sulfate centrifugal flotation technique and nested PCR followed by sequencing. The maximum temperatures measured in the thermophilic phase of the compounds ranged from 55°C to 64.8°C, and at day 280, weight reductions of 27% (T1), 48% (T2), 63% (T3), 66% (T4) and 64% (T5) were observed. The absence of fecal coliforms was observed from day 98 (T4), day 126 (T1), day 196 (T3 and T5) and day 210 (T2). All treatments resulted in helminth egg inviability and the absence of protozoan (oo)cysts. Only T4 and T5 were positive for G. duodenalis according to the sequencing analysis. In conclusion, high temperatures during the composting thermophilic phase made the use of pathogens required by legislation unfeasible; therefore, it is important to investigate the viability of protozoa in compounds to ensure a safe final product for human and animal health.https://jurnalalse.com/wp-content/uploads/ALSE2-2023-01.pdfbiosolidscoliformstemperaturethermotolerant viable helminth eggs
spellingShingle Liza OGAWA
Leopoldo Sussumu MATSUMOTO
Roberta DOS SANTOS TOLEDO
Jonatas CAMPOS DE ALMEIDA
Victor Bittencourt Dutra TABACOW
Fernanda Maria DE OLIVEIRA DIAS
Diego Resende RODRIGUES
Thaís Monica CABRAL
Roberta Lemos FREIRE
Italmar Teodorico NAVARRO
THE EFFECTIVENESS OF COMPOSTING USING STABILIZING URBAN SEWAGE SLUDGE COMPOUNDS
Journal of Applied Life Sciences and Environment
biosolids
coliforms
temperature
thermotolerant viable helminth eggs
title THE EFFECTIVENESS OF COMPOSTING USING STABILIZING URBAN SEWAGE SLUDGE COMPOUNDS
title_full THE EFFECTIVENESS OF COMPOSTING USING STABILIZING URBAN SEWAGE SLUDGE COMPOUNDS
title_fullStr THE EFFECTIVENESS OF COMPOSTING USING STABILIZING URBAN SEWAGE SLUDGE COMPOUNDS
title_full_unstemmed THE EFFECTIVENESS OF COMPOSTING USING STABILIZING URBAN SEWAGE SLUDGE COMPOUNDS
title_short THE EFFECTIVENESS OF COMPOSTING USING STABILIZING URBAN SEWAGE SLUDGE COMPOUNDS
title_sort effectiveness of composting using stabilizing urban sewage sludge compounds
topic biosolids
coliforms
temperature
thermotolerant viable helminth eggs
url https://jurnalalse.com/wp-content/uploads/ALSE2-2023-01.pdf
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