Molecular mechanisms underlying the regulation of tobacco defenses against Bemisia tabaci by the salivary effector BtArmet targeting NtWRKY51
The whitefly Bemisia tabaci, an important agricultural insect pest, secretes saliva into plants while feeding on them. Our previous study found that the whitefly secretes the salivary effector protein BtArmet (Bemisia tabaci arginine rich, mutated in early stage of tumors) to inhibit plant resistanc...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2022-12-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2022.07.111 |
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| _version_ | 1850070101590540288 |
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| author | DU Hui WANG Xiaowei LIU Shusheng |
| author_facet | DU Hui WANG Xiaowei LIU Shusheng |
| author_sort | DU Hui |
| collection | DOAJ |
| description | The whitefly Bemisia tabaci, an important agricultural insect pest, secretes saliva into plants while feeding on them. Our previous study found that the whitefly secretes the salivary effector protein BtArmet (Bemisia tabaci arginine rich, mutated in early stage of tumors) to inhibit plant resistance by targeting tobacco cystatin. In this study, we continued to screen tobacco proteins that interact with the salivary effector protein BtArmet. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays showed that Nicotiana tabacum WRKY51 interacted with BtArmet. The up-regulation of NtWRKY51 gene in tobacco was significantly induced by whitefly infestation. However, virus-induced gene silencing (VIGS) assays combined with bioassay of whiteflies showed that the fecundity of NtWRKY51-silenced tobacco plants by female whiteflies was significantly lower than that of the negative control (tobacco with silenced empty vector). Silencing NtWRKY51 gene had no effect on phytohormone signaling pathways mediated by salicylic acid or jasmonic acid in tobacco plants. These results provide references for further investigations on the molecular mechanisms underlying plant resistance to whiteflies. |
| format | Article |
| id | doaj-art-a5643d3b724441d7b4164e2d1abb35c3 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-a5643d3b724441d7b4164e2d1abb35c32025-08-20T02:47:37ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552022-12-014875376010.3785/j.issn.1008-9209.2022.07.11110089209Molecular mechanisms underlying the regulation of tobacco defenses against Bemisia tabaci by the salivary effector BtArmet targeting NtWRKY51DU HuiWANG XiaoweiLIU ShushengThe whitefly Bemisia tabaci, an important agricultural insect pest, secretes saliva into plants while feeding on them. Our previous study found that the whitefly secretes the salivary effector protein BtArmet (Bemisia tabaci arginine rich, mutated in early stage of tumors) to inhibit plant resistance by targeting tobacco cystatin. In this study, we continued to screen tobacco proteins that interact with the salivary effector protein BtArmet. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays showed that Nicotiana tabacum WRKY51 interacted with BtArmet. The up-regulation of NtWRKY51 gene in tobacco was significantly induced by whitefly infestation. However, virus-induced gene silencing (VIGS) assays combined with bioassay of whiteflies showed that the fecundity of NtWRKY51-silenced tobacco plants by female whiteflies was significantly lower than that of the negative control (tobacco with silenced empty vector). Silencing NtWRKY51 gene had no effect on phytohormone signaling pathways mediated by salicylic acid or jasmonic acid in tobacco plants. These results provide references for further investigations on the molecular mechanisms underlying plant resistance to whiteflies.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2022.07.111whiteflysalivatobaccoplant defenseWRKY51insect-plant interaction |
| spellingShingle | DU Hui WANG Xiaowei LIU Shusheng Molecular mechanisms underlying the regulation of tobacco defenses against Bemisia tabaci by the salivary effector BtArmet targeting NtWRKY51 浙江大学学报. 农业与生命科学版 whitefly saliva tobacco plant defense WRKY51 insect-plant interaction |
| title | Molecular mechanisms underlying the regulation of tobacco defenses against Bemisia tabaci by the salivary effector BtArmet targeting NtWRKY51 |
| title_full | Molecular mechanisms underlying the regulation of tobacco defenses against Bemisia tabaci by the salivary effector BtArmet targeting NtWRKY51 |
| title_fullStr | Molecular mechanisms underlying the regulation of tobacco defenses against Bemisia tabaci by the salivary effector BtArmet targeting NtWRKY51 |
| title_full_unstemmed | Molecular mechanisms underlying the regulation of tobacco defenses against Bemisia tabaci by the salivary effector BtArmet targeting NtWRKY51 |
| title_short | Molecular mechanisms underlying the regulation of tobacco defenses against Bemisia tabaci by the salivary effector BtArmet targeting NtWRKY51 |
| title_sort | molecular mechanisms underlying the regulation of tobacco defenses against bemisia tabaci by the salivary effector btarmet targeting ntwrky51 |
| topic | whitefly saliva tobacco plant defense WRKY51 insect-plant interaction |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2022.07.111 |
| work_keys_str_mv | AT duhui molecularmechanismsunderlyingtheregulationoftobaccodefensesagainstbemisiatabacibythesalivaryeffectorbtarmettargetingntwrky51 AT wangxiaowei molecularmechanismsunderlyingtheregulationoftobaccodefensesagainstbemisiatabacibythesalivaryeffectorbtarmettargetingntwrky51 AT liushusheng molecularmechanismsunderlyingtheregulationoftobaccodefensesagainstbemisiatabacibythesalivaryeffectorbtarmettargetingntwrky51 |