Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications
Aim: Since decades, decellularized extracellular matrix (dECM)-derived materials have received worldwide attention as promising biomaterials for tissue engineering and biomedical applications. Soluble dECM is a versatile raw material that can be easily engineered into the desired shapes and structur...
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Open Exploration Publishing Inc.
2024-04-01
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| Series: | Exploration of BioMat-X |
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| Online Access: | https://www.explorationpub.com/uploads/Article/A10137/10137.pdf |
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| author | Maria Elena Lombardo Valentina Mariscotti Pascale Chevallier Francesco Copes Francesca Boccafoschi Andranik Sarkissian Diego Mantovani |
| author_facet | Maria Elena Lombardo Valentina Mariscotti Pascale Chevallier Francesco Copes Francesca Boccafoschi Andranik Sarkissian Diego Mantovani |
| author_sort | Maria Elena Lombardo |
| collection | DOAJ |
| description | Aim: Since decades, decellularized extracellular matrix (dECM)-derived materials have received worldwide attention as promising biomaterials for tissue engineering and biomedical applications. Soluble dECM is a versatile raw material that can be easily engineered into the desired shapes and structures. However, there are still some limitations restricting its use, including low hydrophilicity and smooth surfaces, which negatively influence cell adhesion/spreading. The objective of the present study was to investigate surface modification by nitrogen/hydrogen (N2/H2) low-pressure cold plasma treatment as a potential technique to improve the biological response of bovine pericardium dECM films. Methods: Bovine pericardium dECM was enzymatically digested and lyophilized prior to the preparation of thin films via solvent-casting method. Changes in surface properties after plasma treatment were investigated using water contact angle (WCA) and X-ray photoelectron spectroscopy (XPS) measurements. Immunofluorescence staining and resazurin assay for human dermal fibroblasts (HDFs) cultured on the dECM films were used to assess the bioactivity of dECM films. Finally, the hemocompatibility of the films was investigated via clotting time and hemolysis assay. Results: WCA and XPS results revealed that oxygen (O)- and N-containing functional groups were incorporated onto the film surface and an increase in hydrophilicity was observed after plasma treatment. In vitro experiments showed that cell adhesion in plasma-treated dECM films is much faster if compared to the untreated controls. Moreover, the fibroblast proliferation increased after plasma surface modifications. Finally, the hemocompatibility analysis results indicated a delayed blood clotting and no hemolytic effects for all the tested samples. Conclusions: These findings confirmed the potential of dECM as raw material for biocompatible thin films fabrication. Additionally, plasma surface treatment emerged as an eco-friendly and cost-effective strategy to enhance in vitro cell attachment and proliferation on dECM films, expanding their applications in biomedicine. |
| format | Article |
| id | doaj-art-a556d455d1724e93b494e83708a9a01f |
| institution | DOAJ |
| issn | 2996-9476 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Open Exploration Publishing Inc. |
| record_format | Article |
| series | Exploration of BioMat-X |
| spelling | doaj-art-a556d455d1724e93b494e83708a9a01f2025-08-20T02:40:32ZengOpen Exploration Publishing Inc.Exploration of BioMat-X2996-94762024-04-0112849910.37349/ebmx.2024.00007Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applicationsMaria Elena Lombardo0https://orcid.org/0000-0003-4685-3503Valentina Mariscotti1Pascale Chevallier2https://orcid.org/0000-0002-0163-9936Francesco Copes3https://orcid.org/0000-0001-6901-971XFrancesca Boccafoschi4https://orcid.org/0000-0002-6734-5447Andranik Sarkissian5https://orcid.org/0000-0003-4051-595XDiego Mantovani6https://orcid.org/0000-0001-9672-895XLaboratory for Biomaterials and Bioengineering, CRC-I, Department of Min-Met-Materials Engineering & CHU de Quebec Research Center, Division of Regenerative Medicine Laval, Laval University, Quebec City, QC G1V0A6, CanadaLaboratory for Biomaterials and Bioengineering, CRC-I, Department of Min-Met-Materials Engineering & CHU de Quebec Research Center, Division of Regenerative Medicine Laval, Laval University, Quebec City, QC G1V0A6, CanadaLaboratory for Biomaterials and Bioengineering, CRC-I, Department of Min-Met-Materials Engineering & CHU de Quebec Research Center, Division of Regenerative Medicine Laval, Laval University, Quebec City, QC G1V0A6, CanadaLaboratory for Biomaterials and Bioengineering, CRC-I, Department of Min-Met-Materials Engineering & CHU de Quebec Research Center, Division of Regenerative Medicine Laval, Laval University, Quebec City, QC G1V0A6, CanadaTissueGraft Inc, 28100 Novara, Italy; Department of Human Anatomy, Faculty of Medicine, University of Oriental Piedmont, 28100 Novara, ItalyPlasmionique Inc, Varennes, QC J3X1S2, CanadaLaboratory for Biomaterials and Bioengineering, CRC-I, Department of Min-Met-Materials Engineering & CHU de Quebec Research Center, Division of Regenerative Medicine Laval, Laval University, Quebec City, QC G1V0A6, CanadaAim: Since decades, decellularized extracellular matrix (dECM)-derived materials have received worldwide attention as promising biomaterials for tissue engineering and biomedical applications. Soluble dECM is a versatile raw material that can be easily engineered into the desired shapes and structures. However, there are still some limitations restricting its use, including low hydrophilicity and smooth surfaces, which negatively influence cell adhesion/spreading. The objective of the present study was to investigate surface modification by nitrogen/hydrogen (N2/H2) low-pressure cold plasma treatment as a potential technique to improve the biological response of bovine pericardium dECM films. Methods: Bovine pericardium dECM was enzymatically digested and lyophilized prior to the preparation of thin films via solvent-casting method. Changes in surface properties after plasma treatment were investigated using water contact angle (WCA) and X-ray photoelectron spectroscopy (XPS) measurements. Immunofluorescence staining and resazurin assay for human dermal fibroblasts (HDFs) cultured on the dECM films were used to assess the bioactivity of dECM films. Finally, the hemocompatibility of the films was investigated via clotting time and hemolysis assay. Results: WCA and XPS results revealed that oxygen (O)- and N-containing functional groups were incorporated onto the film surface and an increase in hydrophilicity was observed after plasma treatment. In vitro experiments showed that cell adhesion in plasma-treated dECM films is much faster if compared to the untreated controls. Moreover, the fibroblast proliferation increased after plasma surface modifications. Finally, the hemocompatibility analysis results indicated a delayed blood clotting and no hemolytic effects for all the tested samples. Conclusions: These findings confirmed the potential of dECM as raw material for biocompatible thin films fabrication. Additionally, plasma surface treatment emerged as an eco-friendly and cost-effective strategy to enhance in vitro cell attachment and proliferation on dECM films, expanding their applications in biomedicine.https://www.explorationpub.com/uploads/Article/A10137/10137.pdfnatural polymersdecellularized extracellular matrixfunctionalizationlow-pressure cold plasmasurface modificationsphysicochemical propertiescell adhesionhemocompatibility |
| spellingShingle | Maria Elena Lombardo Valentina Mariscotti Pascale Chevallier Francesco Copes Francesca Boccafoschi Andranik Sarkissian Diego Mantovani Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications Exploration of BioMat-X natural polymers decellularized extracellular matrix functionalization low-pressure cold plasma surface modifications physicochemical properties cell adhesion hemocompatibility |
| title | Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications |
| title_full | Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications |
| title_fullStr | Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications |
| title_full_unstemmed | Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications |
| title_short | Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications |
| title_sort | effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix based films for biomedical applications |
| topic | natural polymers decellularized extracellular matrix functionalization low-pressure cold plasma surface modifications physicochemical properties cell adhesion hemocompatibility |
| url | https://www.explorationpub.com/uploads/Article/A10137/10137.pdf |
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