Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection.
Although CD8(+) T cells play an important role in the containment of adult HIV-1 replication, their role in infant HIV-1 infection is not as well understood. Impaired HIV-specific CD8(+) T cell responses may underlie the persistently high viral loads observed in infants. We examined the frequency an...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0020375&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849725780357021696 |
|---|---|
| author | Jennifer A Slyker Grace C John-Stewart Tao Dong Barbara Lohman-Payne Marie Reilly Ann Atzberger Stephen Taylor Elizabeth Maleche-Obimbo Dorothy Mbori-Ngacha Sarah L Rowland-Jones |
| author_facet | Jennifer A Slyker Grace C John-Stewart Tao Dong Barbara Lohman-Payne Marie Reilly Ann Atzberger Stephen Taylor Elizabeth Maleche-Obimbo Dorothy Mbori-Ngacha Sarah L Rowland-Jones |
| author_sort | Jennifer A Slyker |
| collection | DOAJ |
| description | Although CD8(+) T cells play an important role in the containment of adult HIV-1 replication, their role in infant HIV-1 infection is not as well understood. Impaired HIV-specific CD8(+) T cell responses may underlie the persistently high viral loads observed in infants. We examined the frequency and phenotype of infant HIV-specific CD8(+) T cells in 7 HIV-infected antiretroviral therapy-naïve infants during the first 2 years of life, using class I HLA tetramers and IFN-γ-ELISPOT. The frequency (0.088-3.9% of CD3(+)CD8(+) cells) and phenotype (CD27(+)CD28(-), CD45RA(+/-), CD57(+/-), HLA-DR(+), CD95(+)) of infant HIV-specific CD8(+) T cells were similar to reports in adults undergoing early infection. Unlike adults, at 23-24 months post-infection a high frequency of HIV-specific CD8(+) T cells expressed HLA-DR (mean 80%, range 68-85%) and CD95 (mean 88%, range 79-96%), suggesting sustained activation and vulnerability to apoptosis. Despite comparable expansion of HIV-specific CD8(+) T cells of a similar phenotype to adults during early infection, infant T cells failed to contain HIV-1 replication, and remained persistently activated and vulnerable to apoptosis during chronic infection. |
| format | Article |
| id | doaj-art-a5507c7d784e4e19ba1cf04774abd50b |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-a5507c7d784e4e19ba1cf04774abd50b2025-08-20T03:10:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e2037510.1371/journal.pone.0020375Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection.Jennifer A SlykerGrace C John-StewartTao DongBarbara Lohman-PayneMarie ReillyAnn AtzbergerStephen TaylorElizabeth Maleche-ObimboDorothy Mbori-NgachaSarah L Rowland-JonesAlthough CD8(+) T cells play an important role in the containment of adult HIV-1 replication, their role in infant HIV-1 infection is not as well understood. Impaired HIV-specific CD8(+) T cell responses may underlie the persistently high viral loads observed in infants. We examined the frequency and phenotype of infant HIV-specific CD8(+) T cells in 7 HIV-infected antiretroviral therapy-naïve infants during the first 2 years of life, using class I HLA tetramers and IFN-γ-ELISPOT. The frequency (0.088-3.9% of CD3(+)CD8(+) cells) and phenotype (CD27(+)CD28(-), CD45RA(+/-), CD57(+/-), HLA-DR(+), CD95(+)) of infant HIV-specific CD8(+) T cells were similar to reports in adults undergoing early infection. Unlike adults, at 23-24 months post-infection a high frequency of HIV-specific CD8(+) T cells expressed HLA-DR (mean 80%, range 68-85%) and CD95 (mean 88%, range 79-96%), suggesting sustained activation and vulnerability to apoptosis. Despite comparable expansion of HIV-specific CD8(+) T cells of a similar phenotype to adults during early infection, infant T cells failed to contain HIV-1 replication, and remained persistently activated and vulnerable to apoptosis during chronic infection.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0020375&type=printable |
| spellingShingle | Jennifer A Slyker Grace C John-Stewart Tao Dong Barbara Lohman-Payne Marie Reilly Ann Atzberger Stephen Taylor Elizabeth Maleche-Obimbo Dorothy Mbori-Ngacha Sarah L Rowland-Jones Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. PLoS ONE |
| title | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_full | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_fullStr | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_full_unstemmed | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_short | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_sort | phenotypic characterization of hiv specific cd8 t cells during early and chronic infant hiv 1 infection |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0020375&type=printable |
| work_keys_str_mv | AT jenniferaslyker phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection AT gracecjohnstewart phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection AT taodong phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection AT barbaralohmanpayne phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection AT mariereilly phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection AT annatzberger phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection AT stephentaylor phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection AT elizabethmalecheobimbo phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection AT dorothymboringacha phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection AT sarahlrowlandjones phenotypiccharacterizationofhivspecificcd8tcellsduringearlyandchronicinfanthiv1infection |