Pathogenic mucorales: Deciphering their cell wall polysaccharidome and immunostimulatory potential
Mucormycosis is an emerging infection caused by pathogenic filamentous fungal species belonging to the Order Mucorales. Mortality associated with mucormycosis is significantly high in patients with compromised immunity. As cell wall is the first fungal component to interact with the host immune syst...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2528079 |
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| author | Mathieu Lepas Julia Marcondes Figueiredo Sarah Dellière Sarah Sze Wah Wong Dea Garcia-Hermoso Magalie Duchateau Mariette Matondo Fanny Lanternier Vishukumar Aimanianda |
| author_facet | Mathieu Lepas Julia Marcondes Figueiredo Sarah Dellière Sarah Sze Wah Wong Dea Garcia-Hermoso Magalie Duchateau Mariette Matondo Fanny Lanternier Vishukumar Aimanianda |
| author_sort | Mathieu Lepas |
| collection | DOAJ |
| description | Mucormycosis is an emerging infection caused by pathogenic filamentous fungal species belonging to the Order Mucorales. Mortality associated with mucormycosis is significantly high in patients with compromised immunity. As cell wall is the first fungal component to interact with the host immune system, we characterized cell wall organization and composition of the three most prevalent pathogenic species of Mucorales, Rhizopus arrhizus, Mucor circinelloides, and Lichtheimia corymbifera and studied their immunomodulatory potential. Staining and lectin-/immunolabeling indicated that the spores and germ-tubes of these three Mucorales species have surface-exposed mannans, while germ-tubes showed distinctly distributed β-1,3-glucan. Gas chromatography analysis of the cell wall indicated that glucose polymer is the major fibrillar polysaccharide present in the three species, whereas amorphous components were species-dependent. Specific enzymatic digestion followed by chromatography analysis indicated that β-1,3-glucan, β-1,6-glucan, and amylase digestible glucan constitute firbrillar polysaccharides. Stimulation of human peripheral blood mononuclear cells or THP-1 (human leukemic monocytic) cells with spores or extracted cell wall polysaccharides resulted in the release of pro-inflammatory cytokines regardless of the Mucorales species. Together, the Mucorales species analyzed in this study show a common and species-specific cell wall composition. The cell wall polysaccharides are highly pro-inflammatory, suggesting that undue or excessive inflammation may contribute to the immunopathology of mucormycosis. |
| format | Article |
| id | doaj-art-a53bd58c4a094ceb902e074dae1d403c |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-a53bd58c4a094ceb902e074dae1d403c2025-08-20T03:30:36ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2528079Pathogenic mucorales: Deciphering their cell wall polysaccharidome and immunostimulatory potentialMathieu Lepas0Julia Marcondes Figueiredo1Sarah Dellière2Sarah Sze Wah Wong3Dea Garcia-Hermoso4Magalie Duchateau5Mariette Matondo6Fanny Lanternier7Vishukumar Aimanianda8Institut Pasteur, Université Paris Cité, Molecular Mycology Unit, UMR CNRS2000, Paris, FranceInstitut Pasteur, Université Paris Cité, Immunobiology of Aspergillus, Paris, FranceInstitut Pasteur, Université Paris Cité, Molecular Mycology Unit, UMR CNRS2000, Paris, FranceInstitut Pasteur, Université Paris Cité, Molecular Mycology Unit, UMR CNRS2000, Paris, FranceMycology Department, Institut Pasteur, Université Paris Cité, National Reference Center for Invasive Mycoses and Antifungals, Translational Mycology research group, Paris, FranceInstitut Pasteur, Proteomics Platform, Mass Spectrometry for Biology Unit, CNRS URA 2024, Paris, FranceInstitut Pasteur, Proteomics Platform, Mass Spectrometry for Biology Unit, CNRS URA 2024, Paris, FranceMycology Department, Institut Pasteur, Université Paris Cité, National Reference Center for Invasive Mycoses and Antifungals, Translational Mycology research group, Paris, FranceInstitut Pasteur, Université Paris Cité, Molecular Mycology Unit, UMR CNRS2000, Paris, FranceMucormycosis is an emerging infection caused by pathogenic filamentous fungal species belonging to the Order Mucorales. Mortality associated with mucormycosis is significantly high in patients with compromised immunity. As cell wall is the first fungal component to interact with the host immune system, we characterized cell wall organization and composition of the three most prevalent pathogenic species of Mucorales, Rhizopus arrhizus, Mucor circinelloides, and Lichtheimia corymbifera and studied their immunomodulatory potential. Staining and lectin-/immunolabeling indicated that the spores and germ-tubes of these three Mucorales species have surface-exposed mannans, while germ-tubes showed distinctly distributed β-1,3-glucan. Gas chromatography analysis of the cell wall indicated that glucose polymer is the major fibrillar polysaccharide present in the three species, whereas amorphous components were species-dependent. Specific enzymatic digestion followed by chromatography analysis indicated that β-1,3-glucan, β-1,6-glucan, and amylase digestible glucan constitute firbrillar polysaccharides. Stimulation of human peripheral blood mononuclear cells or THP-1 (human leukemic monocytic) cells with spores or extracted cell wall polysaccharides resulted in the release of pro-inflammatory cytokines regardless of the Mucorales species. Together, the Mucorales species analyzed in this study show a common and species-specific cell wall composition. The cell wall polysaccharides are highly pro-inflammatory, suggesting that undue or excessive inflammation may contribute to the immunopathology of mucormycosis.https://www.tandfonline.com/doi/10.1080/21505594.2025.2528079Mucoralesmucormycosiscell wallpolysaccharidesβ-glucanimmunomodulation |
| spellingShingle | Mathieu Lepas Julia Marcondes Figueiredo Sarah Dellière Sarah Sze Wah Wong Dea Garcia-Hermoso Magalie Duchateau Mariette Matondo Fanny Lanternier Vishukumar Aimanianda Pathogenic mucorales: Deciphering their cell wall polysaccharidome and immunostimulatory potential Virulence Mucorales mucormycosis cell wall polysaccharides β-glucan immunomodulation |
| title | Pathogenic mucorales: Deciphering their cell wall polysaccharidome and immunostimulatory potential |
| title_full | Pathogenic mucorales: Deciphering their cell wall polysaccharidome and immunostimulatory potential |
| title_fullStr | Pathogenic mucorales: Deciphering their cell wall polysaccharidome and immunostimulatory potential |
| title_full_unstemmed | Pathogenic mucorales: Deciphering their cell wall polysaccharidome and immunostimulatory potential |
| title_short | Pathogenic mucorales: Deciphering their cell wall polysaccharidome and immunostimulatory potential |
| title_sort | pathogenic mucorales deciphering their cell wall polysaccharidome and immunostimulatory potential |
| topic | Mucorales mucormycosis cell wall polysaccharides β-glucan immunomodulation |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2528079 |
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