Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patients
Introduction Non-Hodgkin lymphomas (NHLs) are the most common hematological malignancies worldwide. Among these, B-cell lymphomas (B-NHLs) are the second leading cause of death in hematologic neoplasms.Material and methods In this study, a detailed immunophenotypic analysis of lymphocytes in the bon...
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Taylor & Francis Group
2025-12-01
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| Series: | Annals of Medicine |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07853890.2025.2490825 |
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| author | Luciana Valvano Rocchina Vilella Fiorella D’Auria Giovanni D’Arena Rossana Libonati Michela Soda Alessia Telesca Giuseppe Pietrantuono Giovanna Rosaria Mansueto Oreste Villani Simona D’Agostino Giovanni Calice Teodora Statuto |
| author_facet | Luciana Valvano Rocchina Vilella Fiorella D’Auria Giovanni D’Arena Rossana Libonati Michela Soda Alessia Telesca Giuseppe Pietrantuono Giovanna Rosaria Mansueto Oreste Villani Simona D’Agostino Giovanni Calice Teodora Statuto |
| author_sort | Luciana Valvano |
| collection | DOAJ |
| description | Introduction Non-Hodgkin lymphomas (NHLs) are the most common hematological malignancies worldwide. Among these, B-cell lymphomas (B-NHLs) are the second leading cause of death in hematologic neoplasms.Material and methods In this study, a detailed immunophenotypic analysis of lymphocytes in the bone marrow aspirate (BMA) of 75 patients with four different subtypes of B-NHLs was performed at diagnosis. The samples were analyzed by flow cytometry (FC) using a stain-lyse-no wash technique and a comprehensive six-color antibody panel.Results Our data showed a different trend in the percentage values of the distinct lymphocyte subsets, which did not seem to correlate with a worse prognosis, except for B cells in diffuse large B-cell lymphoma (DLBCL), which were significantly higher in stage IV than in stages II and III. ROC curve analysis showed that the B-cell percentage value could be used to predict the stage of the disease. Total lymphocytes and B cells were greater in lymphomas that presented a lower percentage of disease progression, specifically mantle cell lymphoma (MCL) and marginal zone lymphoma (MZL). In contrast, natural killer (NK) and T cells showed higher values in DLBCL and follicular lymphoma (FL), which progressed more frequently. Interestingly, in DLBCL patients with higher percentage values of double positive (DPT) and helper T cells (Th), we observed a good prognosis. Specifically, univariate Cox regression analyses indicated that a higher value of Th cells at diagnosis was a better prognostic predictor in patients with DLBCL.Conclusions These preliminary findings encourage us to further investigate the role of lymphocyte subpopulations in B-cell NHL. |
| format | Article |
| id | doaj-art-a52cd8b66c2b4659912b2cf29cc5709f |
| institution | OA Journals |
| issn | 0785-3890 1365-2060 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Medicine |
| spelling | doaj-art-a52cd8b66c2b4659912b2cf29cc5709f2025-08-20T02:16:14ZengTaylor & Francis GroupAnnals of Medicine0785-38901365-20602025-12-0157110.1080/07853890.2025.2490825Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patientsLuciana Valvano0Rocchina Vilella1Fiorella D’Auria2Giovanni D’Arena3Rossana Libonati4Michela Soda5Alessia Telesca6Giuseppe Pietrantuono7Giovanna Rosaria Mansueto8Oreste Villani9Simona D’Agostino10Giovanni Calice11Teodora Statuto12Laboratory of Clinical Research and Advanced Diagnostics, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyLaboratory of Clinical Research and Advanced Diagnostics, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyLaboratory of Clinical Pathology, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyHematology, “S. Luca” Hospital, ASL Salerno, ItalyLaboratory of Clinical Research and Advanced Diagnostics, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyLaboratory of Clinical Research and Advanced Diagnostics, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyLaboratory of Clinical Research and Advanced Diagnostics, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyHematology and Stem Cell Transplantation Unit, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyHematology and Stem Cell Transplantation Unit, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyHematology and Stem Cell Transplantation Unit, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyHematology and Stem Cell Transplantation Unit, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyLaboratory of Preclinical and Translational Research, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyLaboratory of Clinical Research and Advanced Diagnostics, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, ItalyIntroduction Non-Hodgkin lymphomas (NHLs) are the most common hematological malignancies worldwide. Among these, B-cell lymphomas (B-NHLs) are the second leading cause of death in hematologic neoplasms.Material and methods In this study, a detailed immunophenotypic analysis of lymphocytes in the bone marrow aspirate (BMA) of 75 patients with four different subtypes of B-NHLs was performed at diagnosis. The samples were analyzed by flow cytometry (FC) using a stain-lyse-no wash technique and a comprehensive six-color antibody panel.Results Our data showed a different trend in the percentage values of the distinct lymphocyte subsets, which did not seem to correlate with a worse prognosis, except for B cells in diffuse large B-cell lymphoma (DLBCL), which were significantly higher in stage IV than in stages II and III. ROC curve analysis showed that the B-cell percentage value could be used to predict the stage of the disease. Total lymphocytes and B cells were greater in lymphomas that presented a lower percentage of disease progression, specifically mantle cell lymphoma (MCL) and marginal zone lymphoma (MZL). In contrast, natural killer (NK) and T cells showed higher values in DLBCL and follicular lymphoma (FL), which progressed more frequently. Interestingly, in DLBCL patients with higher percentage values of double positive (DPT) and helper T cells (Th), we observed a good prognosis. Specifically, univariate Cox regression analyses indicated that a higher value of Th cells at diagnosis was a better prognostic predictor in patients with DLBCL.Conclusions These preliminary findings encourage us to further investigate the role of lymphocyte subpopulations in B-cell NHL.https://www.tandfonline.com/doi/10.1080/07853890.2025.2490825B-cell non-Hodgkin lymphomaDLBCLimmune cellsB cellsTh cellsdouble positive T-cells |
| spellingShingle | Luciana Valvano Rocchina Vilella Fiorella D’Auria Giovanni D’Arena Rossana Libonati Michela Soda Alessia Telesca Giuseppe Pietrantuono Giovanna Rosaria Mansueto Oreste Villani Simona D’Agostino Giovanni Calice Teodora Statuto Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patients Annals of Medicine B-cell non-Hodgkin lymphoma DLBCL immune cells B cells Th cells double positive T-cells |
| title | Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patients |
| title_full | Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patients |
| title_fullStr | Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patients |
| title_full_unstemmed | Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patients |
| title_short | Prognostic relevance of bone marrow immune cell fractions in newly diagnosed B-cell non-Hodgkin lymphoma patients |
| title_sort | prognostic relevance of bone marrow immune cell fractions in newly diagnosed b cell non hodgkin lymphoma patients |
| topic | B-cell non-Hodgkin lymphoma DLBCL immune cells B cells Th cells double positive T-cells |
| url | https://www.tandfonline.com/doi/10.1080/07853890.2025.2490825 |
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