Prevention of Muscle Atrophy by Low-Molecular-Weight Fraction from <i>Hirsutella sinensis</i> Mycelium
Muscle atrophy, an age-related condition, presents a growing healthcare concern within the context of global population aging. While studies have investigated <i>Hirsutella sinensis</i> for its potential antifatigue properties, reports on its active components remain limited. This study...
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2024-12-01
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| author | Yi-Wen Chen Tsung-Ju Li Li-Ching Wang Bi-Hua Yang Yen-Lien Chen Chin-Chu Chen Hsin-Tang Lin |
| author_facet | Yi-Wen Chen Tsung-Ju Li Li-Ching Wang Bi-Hua Yang Yen-Lien Chen Chin-Chu Chen Hsin-Tang Lin |
| author_sort | Yi-Wen Chen |
| collection | DOAJ |
| description | Muscle atrophy, an age-related condition, presents a growing healthcare concern within the context of global population aging. While studies have investigated <i>Hirsutella sinensis</i> for its potential antifatigue properties, reports on its active components remain limited. This study evaluated the efficacy of <i>H. sinensis</i> mycelium extract on muscle health, utilizing a 1:1 water–ethanol preparation administered to C57BL/6 mice exhibiting acute hind leg atrophy. The results indicated no adverse effects, with significant improvements in muscle endurance and soleus muscle mass observed over a 14-day period. To further elucidate the mechanisms and effects of <i>H. sinensis</i> mycelium on dexamethasone-induced muscle atrophy, the water extract was fractionated into components of <3.5 kDa, 3.5–10 kDa, and >10 kDa using dialysis membranes. The investigation utilized a C2C12 cell atrophy model, induced by dexamethasone, to analyze the expression of relevant genes via qPCR. The results demonstrated that the <3.5 kDa and >10 kDa fractions significantly upregulated the expression of <i>Myh2</i> and <i>Myh7</i> genes while simultaneously downregulating the expression of <i>MuRF-1</i> and <i>Atrogin-1</i>. It is noteworthy that the <3.5 kDa fraction exclusively enhanced MYHC protein expression and suppressed AMPK expression, as confirmed by Western blot analysis. This comprehensive pilot study suggests that the low-molecular-weight fraction of <i>H. sinensis</i> mycelium exhibits considerable potential for muscle mass preservation and atrophy mitigation. As a result, it offers a promising direction for the development of supplements aimed at addressing fatigue and preventing muscle atrophy. |
| format | Article |
| id | doaj-art-a508b2f61df448bdb53b96554b9596d1 |
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| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-a508b2f61df448bdb53b96554b9596d12025-08-20T02:53:19ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-12-014612140331404410.3390/cimb46120839Prevention of Muscle Atrophy by Low-Molecular-Weight Fraction from <i>Hirsutella sinensis</i> MyceliumYi-Wen Chen0Tsung-Ju Li1Li-Ching Wang2Bi-Hua Yang3Yen-Lien Chen4Chin-Chu Chen5Hsin-Tang Lin6Biotech Research Institute, Grape King Bio Ltd., Taoyuan City 325, TaiwanBiotech Research Institute, Grape King Bio Ltd., Taoyuan City 325, TaiwanDepartment of Food Safety, National Chung Hsing University, Taichung City 402, TaiwanBiotech Research Institute, Grape King Bio Ltd., Taoyuan City 325, TaiwanBiotech Research Institute, Grape King Bio Ltd., Taoyuan City 325, TaiwanBiotech Research Institute, Grape King Bio Ltd., Taoyuan City 325, TaiwanDepartment of Food Safety, National Chung Hsing University, Taichung City 402, TaiwanMuscle atrophy, an age-related condition, presents a growing healthcare concern within the context of global population aging. While studies have investigated <i>Hirsutella sinensis</i> for its potential antifatigue properties, reports on its active components remain limited. This study evaluated the efficacy of <i>H. sinensis</i> mycelium extract on muscle health, utilizing a 1:1 water–ethanol preparation administered to C57BL/6 mice exhibiting acute hind leg atrophy. The results indicated no adverse effects, with significant improvements in muscle endurance and soleus muscle mass observed over a 14-day period. To further elucidate the mechanisms and effects of <i>H. sinensis</i> mycelium on dexamethasone-induced muscle atrophy, the water extract was fractionated into components of <3.5 kDa, 3.5–10 kDa, and >10 kDa using dialysis membranes. The investigation utilized a C2C12 cell atrophy model, induced by dexamethasone, to analyze the expression of relevant genes via qPCR. The results demonstrated that the <3.5 kDa and >10 kDa fractions significantly upregulated the expression of <i>Myh2</i> and <i>Myh7</i> genes while simultaneously downregulating the expression of <i>MuRF-1</i> and <i>Atrogin-1</i>. It is noteworthy that the <3.5 kDa fraction exclusively enhanced MYHC protein expression and suppressed AMPK expression, as confirmed by Western blot analysis. This comprehensive pilot study suggests that the low-molecular-weight fraction of <i>H. sinensis</i> mycelium exhibits considerable potential for muscle mass preservation and atrophy mitigation. As a result, it offers a promising direction for the development of supplements aimed at addressing fatigue and preventing muscle atrophy.https://www.mdpi.com/1467-3045/46/12/839<i>Hirsutella sinensis</i> myceliumsarcopeniamuscle atrophyC2C12 cell |
| spellingShingle | Yi-Wen Chen Tsung-Ju Li Li-Ching Wang Bi-Hua Yang Yen-Lien Chen Chin-Chu Chen Hsin-Tang Lin Prevention of Muscle Atrophy by Low-Molecular-Weight Fraction from <i>Hirsutella sinensis</i> Mycelium Current Issues in Molecular Biology <i>Hirsutella sinensis</i> mycelium sarcopenia muscle atrophy C2C12 cell |
| title | Prevention of Muscle Atrophy by Low-Molecular-Weight Fraction from <i>Hirsutella sinensis</i> Mycelium |
| title_full | Prevention of Muscle Atrophy by Low-Molecular-Weight Fraction from <i>Hirsutella sinensis</i> Mycelium |
| title_fullStr | Prevention of Muscle Atrophy by Low-Molecular-Weight Fraction from <i>Hirsutella sinensis</i> Mycelium |
| title_full_unstemmed | Prevention of Muscle Atrophy by Low-Molecular-Weight Fraction from <i>Hirsutella sinensis</i> Mycelium |
| title_short | Prevention of Muscle Atrophy by Low-Molecular-Weight Fraction from <i>Hirsutella sinensis</i> Mycelium |
| title_sort | prevention of muscle atrophy by low molecular weight fraction from i hirsutella sinensis i mycelium |
| topic | <i>Hirsutella sinensis</i> mycelium sarcopenia muscle atrophy C2C12 cell |
| url | https://www.mdpi.com/1467-3045/46/12/839 |
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